We narrowed to 23,163 results for: crispr

1. pC011 LwCas13a locus with targeting spacer

   Plasmid

   #91903

   Purpose

   LwCas13a locus into pACYC184 with targeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LwCas13a with targeting spacer
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. MLKL gRNA (BRDN0001149268)

   Plasmid

   #77541

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MLKL

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MLKL (MLKL Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

   Plasmid

   #113039

   Purpose

   AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

   Plasmid

   #100716

   Purpose

   B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting ATP1A1 (cloned using BsmBI)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoters

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. NEK7 gRNA (BRDN0001162196)

   Plasmid

   #77740

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NEK7

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NEK7 (NEK7 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. non-targeting control gRNA (BRDN0001148500)

   Plasmid

   #80260

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDicAID_nCas9-PmCDA-2A-NptII_ETR

   Plasmid

   #91695

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB_EF1a-CasRx-msfGFP-2A-Blast

   Plasmid

   #226010

   Purpose

   CasRx protein for RNA targeting in piggyBac backbone

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Insert

   CasRx RNA-targeting nuclease
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRIM33 gRNA (BRDN0001162242)

   Plasmid

   #77099

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM33

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM33 (TRIM33 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRIM24 gRNA (BRDN0001146855)

    Plasmid

    #77886

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM24

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAC149-pCR8-dCas9VP160

    Plasmid

    #48221

    Purpose

    dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP160

    Mutation

    D10A;H840A nuclease-deficient

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRIM33 gRNA (BRDN0001162291)

    Plasmid

    #77097

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM33

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM33 (TRIM33 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TRIM33 gRNA (BRDN0001162239)

    Plasmid

    #77098

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM33

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM33 (TRIM33 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PIK3C2B gRNA (BRDN0001145952)

    Plasmid

    #76714

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. UAS-u(S)Cas9

    Plasmid

    #127383

    Purpose

    Expresses Cas9 at high level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(S)-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAST

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHelper-T7IS1

    Plasmid

    #140631

    Purpose

    Expresses ShCAST under the T7 promoter and an empty sgRNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6-sgGAL4-1

    Plasmid

    #46915

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgGAL4-1
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRIM24 gRNA (BRDN0001145637)

    Plasmid

    #77887

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM24

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187950

    Purpose

    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RPS6KA1 gRNA (BRDN0001145974)

    Plasmid

    #75497

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA1 (RPS6KA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

    Plasmid

    #136382

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-VPR
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D570A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pROS11

    Plasmid

    #107925

    Purpose

    amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJZC33

    Plasmid

    #62330

    Purpose

    sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2 binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TK1 gRNA (BRDN0001145069)

    Plasmid

    #76424

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TK1 (TK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. RPS6KA1 gRNA (BRDN0001148103)

    Plasmid

    #75498

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA1 (RPS6KA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187948

    Purpose

    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMEL16

    Plasmid

    #107922

    Purpose

    HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNOC_hfnCas12a_NR1_NR2

    Plasmid

    #176252

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by full

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

    Plasmid

    #113677

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82702

    Purpose

    AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    H1/TO Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. RPS6KA2 gRNA (BRDN0001148851)

    Plasmid

    #75734

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA2 (RPS6KA2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCfB3052(gRNA X-4, XI-3, XII-5)

    Plasmid

    #73294

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid

    #140004

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. NUAK1 gRNA (BRDN0001162598)

    Plasmid

    #75577

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TBK1 gRNA (BRDN0001148392)

    Plasmid

    #76362

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TBK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TBK1 (TBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187951

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA

    Plasmid

    #113686

    Purpose

    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    KRAB

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid

    #48227

    Purpose

    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    

    Use

    CRISPR

    Tags

    2A-neo, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RPS6KA2 gRNA (BRDN0001147334)

    Plasmid

    #75732

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA2 (RPS6KA2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEF004-sgRNA-shuffle-in

    Plasmid

    #104440

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    U6 promoter

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMonAID_dCas9-PmCDA_Hyg_ALS

    Plasmid

    #91692

    Purpose

    Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A and H840A for dead Cas9

    Promoter

    2x35S

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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42. pLCKO_LacZ_sgRNA

    Plasmid

    #74179

    Purpose

    lentiviral vector expressing sgRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    LacZ sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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43. NUAK1 gRNA (BRDN0001147733)

    Plasmid

    #75576

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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44. NUAK1 gRNA (BRDN0001162525)

    Plasmid

    #75578

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. TBK1 gRNA (BRDN0001145663)

    Plasmid

    #76361

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TBK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TBK1 (TBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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46. pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

    Plasmid

    #113681

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. PKN1 gRNA (BRDN0001162558)

    Plasmid

    #76278

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN1 (PKN1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

    Plasmid

    #105036

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. PKN3 gRNA (BRDN0001146586)

    Plasmid

    #77947

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. PHKG1 gRNA (BRDN0001144981)

    Plasmid

    #76356

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PHKG1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PHKG1 (PHKG1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge