159,238 results

1. pCMV-MMLVgag-3xNES-ABE8e-NG

   Plasmid

   #181754

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e-NG
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lamp1-mNeonGreen

   Plasmid

   #98882

   Purpose

   In vivo visualization of lysosomes (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   Lamp1
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET StrepII TEV LIC cloning vector (1R)

   Plasmid

   #29664

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Strep II and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. MP1

   Plasmid

   #69627

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 umuD' umuC recA730
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

   Plasmid

   #158653

   Purpose

   making transgenics expressing a UAS driven mCherry and NTR 2.0

   Depositor

   Jeff Mumm

   Article

   Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

   Insert

   5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
   

   Use

   Tol2-based transgenic expression in zebrafish/oth…

   Tags

   Expression

   Mutation

   F70A/F108Y

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-SFFV-GFP1-10

   Plasmid

   #80409

   Purpose

   Expresses GFP1-10 in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP1-10
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1-MAT2A

   Plasmid

   #100521

   Purpose

   Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   MAT2A (MAT2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTight TRE

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-NLS-HA-Bxb1

   Plasmid

   #51271

   Purpose

   Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS-HA-Bxb1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 236HSC WT (BRCA2)

   Plasmid

   #16246

   Depositor

   Mien-Chie Hung

   Article

   Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

   Insert

   BRCA2 (BRCA2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAVS1 T2 CRIPR in pX330

    Plasmid

    #72833

    Purpose

    CRISPR/Cas to target the AAVS1 locus in human cells

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Cas9 and gRNA for targeting the AAVS1 locus in human cells
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-IP-HaloTag7-LC3

    Plasmid

    #184899

    Purpose

    Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-myc-CUL3

    Plasmid

    #19893

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    3xmyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOG44_FLP_recombinase (pEHA1338)

    Plasmid

    #209087

    Purpose

    FLP recombinase for FRT-mediated integration

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Insert

    FLP recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-CD81-10

    Plasmid

    #55012

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD81 (CD81 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET/ELP-I-CAT

    Plasmid

    #71461

    Purpose

    ELP-intein purification system

    Depositor

    David Wood

    Article

    Banki et al Nat Methods. 2005 Sep;2(9):659-61.

    Type

    Empty backbone

    Use

    Tags

    ELP ((VPGXG)110) and intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. ATG9A-mCherry

    Plasmid

    #197393

    Purpose

    Expresses ATG9A in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-FGFR3-S249C

    Plasmid

    #116386

    Purpose

    Lentiviral expression of FGFR3 S249C

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3 (FGFR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S249C

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSR43.6r

    Plasmid

    #63197

    Purpose

    Expresses CcaS, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti‐Cas9‐2A‐Blast

    Plasmid

    #73310

    Purpose

    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_CMV: Flag-Akaluc_SV40:PuroR

    Plasmid

    #183048

    Purpose

    Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    AkaLuc
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge