Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

169,603 results

Showing: 5251 - 5300 of 169603 results

  5. pDNR-3xFLAG-T2A-3xPuroR-Frame 0

    Plasmid
    #245866
    Purpose
    HITAG Donor plasmid for Frame 0 with 3xFLAG tag and triple Puromycin resistant marker (3xPuroR)
    Depositor
    Alejandro Chavez
    Insert
    puromycin resistant marker
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    Endogenous gene promoter after knock-in
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245866-plasmid-map-sequence-id-491961

    Enlarge

  6. pDNR-3xFLAG-T2A-3xPuroR-Frame 1

    Plasmid
    #245867
    Purpose
    HITAG Donor plasmid for Frame 1 with 3xFLAG tag and triple Puromycin resistant marker (3xPuroR)
    Depositor
    Alejandro Chavez
    Insert
    ampicilin resistant marker
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245867-plasmid-map-sequence-id-491963

    Enlarge

  7. pDNR-3xFLAG-T2A-3xPuroR-Frame 2

    Plasmid
    #245868
    Purpose
    HITAG Donor plasmid for Frame 2 with 3xFLAG tag and triple Puromycin resistant marker (3xPuroR)
    Depositor
    Alejandro Chavez
    Insert
    3xFLAG
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    245868-plasmid-map-sequence-id-491964

    Enlarge

  12. pFN1-Aad9

    Plasmid
    #244449
    Purpose
    A shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a spectinomycin resistance marker (aadA gene).
    Depositor
    Xue Liu
    Inserts
    pFn1 Fn ori
    Aad9
    Expression
    Bacterial
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    244449-plasmid-map-sequence-id-490024

    Enlarge

  14. pBI121::smRatioGFP:AQ

    Plasmid
    #240453
    Purpose
    Expression of indicator protein fusion (soluble modified ratiometric pHluorin & Aequorin) for monitoring calcium concentrations and pH in the cytoplasm of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of soluble modified ratiometric pHluorin and Aequorin
    Expression
    Bacterial and Plant
    Mutation
    Ratiometric GFP (ratiometric pHluorin) (PMID 9671…
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240453-plasmid-map-sequence-id-485325

    Enlarge

  15. pBI121::chitEclipGFP:LAAQ

    Plasmid
    #240452
    Purpose
    Expression of indicator protein fusion (modified ecliptic pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of Chitinase signal, soluble modified ecliptic pHluorin and low affinity Aequorin (D119A)
    Expression
    Bacterial and Plant
    Mutation
    Ecliptic GFP (ecliptic pHluorin) (PMID 9671304); …
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240452-plasmid-map-sequence-id-485324

    Enlarge

  20. pcDNA3-Par6-ExRai-CKAR2

    Plasmid
    #236101
    Purpose
    Subcellularly targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells for selective detection of aPKC activity within the Par complex.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    Par6C-ExRai-CKAR2 (PARD6A Synthetic, Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236101-plasmid-map-sequence-id-473796

    Enlarge

  28. TFORF3355

    Plasmid
    #144831
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXN2 (FOXN2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144831-plasmid-map-sequence-id-404422

    Enlarge

  31. AiP12556 - pAAV-AiE0410m_3xC-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2556)

    Plasmid
    #208144
    Purpose
    AiE0410m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain oligodendrocytes
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Mutation
    NA
    Promoter
    Beta Globin minimal promoter
    Available Since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    208144-plasmid-map-sequence-id-410963

    Enlarge

  36. PX551

    Plasmid
    #60957
    Purpose
    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    pMecp2
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60957-plasmid-map-sequence-id-181490

    Enlarge

  39. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68417-plasmid-map-sequence-id-120195

    Enlarge

  50. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190033
    Purpose
    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13X
    Expression
    Mammalian
    Mutation
    Y672A,Y676A
    Promoter
    CBh, SV40, hU6
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190033-plasmid-map-sequence-id-488812

    Enlarge

Showing: 5251 - 5300 of 169603 results