173,847 results
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Plasmid#217330PurposeLentiviral expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPR and LentiviralTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2MutationR1226A/E174R/S542R/K548RPromoterUCOE-SFFVAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2
Plasmid#112669PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.DepositorInserttdT
UseAAV; Donor plasmid for recombinase-mediated casse…ExpressionMammalianAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G1-Ph
Plasmid#46814PurposeTEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneTagsFLAG and c-mycExpressionYeastPromoterTEF1 and PGK1Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRRLsin-SV40 T antigen-IRES-mCherry
Plasmid#58993Purposelentiviral expression of SV40 large T antigen and mCherry red fluorescent reporterDepositorInsertsCMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein
UseLentiviralExpressionMammalianMutationEcoRV site in MCS converted to BamHI sitePromoterCMV enhancer-chicken beta actinAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-TOMM20-N-10
Plasmid#55146PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
PT3.5/CMV-EGFRvIII
Plasmid#20280DepositorAvailable SinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLX401-INK4A
Plasmid#121919PurposeDoxycycline-inducible expression of p16-INK4A product of CDKN2ADepositorAvailable SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIVT-eeBxb1
Plasmid#232748PurposeIVT template for making modRNA encoding eeBxb1DepositorInsertBxb1
UseIn vitro transcriptionTagsHA and SV40 NLSMutationV74A, E229K, V375IAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV CHMP2B-3XHA
Plasmid#232002PurposeExpression of CHMP2B with a 3xHA tag.DepositorAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCFD4-U6:1_U6:3tandemgRNAs
Plasmid#49411Purposeexpression of two gRNAs from Drosophila U6:1 and U6:3DepositorInsertsdU6-1:gRNA
dU6-3:gRNA
UseCRISPRExpressionInsectPromoterdU6-1 and dU6-3Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NORAD
Plasmid#120383PurposeExpression vector for lncRNA NORADDepositorAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
TagsbdSUMOExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFYF1320 EGFP Site#1
Plasmid#47511Purposehuman gRNA expression vector targeting EGFPDepositorInsertgRNA-EGFP site 1
UseCRISPRExpressionMammalianPromoterU6Available SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a
Plasmid#80611PurposepBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)
Plasmid#86420PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoterDepositorInsertCDC42pr-GFP1-10
TagsGFP1-10ExpressionYeastAvailable SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti.Cas9.BFP.Blast
Plasmid#196714PurposeWT SpCas9 with BSD-TagBFP for selection and sortingDepositorInsertCas9-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterEF1aAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-mCD8
Plasmid#162747PurposeNFAT-driven ZsGreen-1 reporter gene with mouse CD8 geneDepositorInsert8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
UseRetroviralExpressionMammalianAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti.DTR.GFP
Plasmid#201962PurposeLentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFPDepositorInsertDiptheria Toxin Receptor
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1alphaAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_tdTomato
Plasmid#198041PurposeBxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.DepositorInserttdTomato
UseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Lck-mScarlet3
Plasmid#234540Purposeto label the membrane of cells with a fluorescent protein (mScarlet3)DepositorInsertLck-mScarlet3
UseAAVMutationNonePromoterShort CAGAvailable SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre-T2A-mRuby2
Plasmid#102989PurposeExpresses Cre and mRuby2DepositorInsertsCre (site-specific recombinase)
mRuby2
UseCre/LoxExpressionMammalianPromoterCAGAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIE1
Plasmid#136355PurposeSelection by Inducible EffectorsDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedMutationATG removed to add signal sequence before genePromoterP1TDP-GHO23Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab5B
Plasmid#61802PurposeHuman Rab5B fluorescently tagged with GFP on the N-terminus for mammalian expressionDepositorAvailable SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3XMyc-EGFP-OMP25
Plasmid#83355PurposeFor tagging mitochondria with Myc epitopes (Control-MITO construct)DepositorInsert3XMyc-EGFP-OMP25
UseRetroviralExpressionMammalianAvailable SinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pComb3XSS
Plasmid#63890Purpose3rd generation plasmid for phage display on modified geneIII, contains stuffer fragmentDepositorInsertsSS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer
UsePhage displayTags6x His, HA tag, and geneIIIExpressionBacterialPromoterlacZAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)
Plasmid#86413Purposenuclear split GFP11 reporter with mCherryDepositorInsertNop1pr-GFP11-mCherry-PUS1
TagsGFP11 and mCherryExpressionYeastPromoterNOP1Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GfaABC1D.PI.Lck-GFP.SV40
Plasmid#105598PurposeAAV expression of membrane bound EGFP from GfaABC1D promoterDepositorHas ServiceAAV5InsertLck-EGFP
UseAAVExpressionMammalianPromoterGfaABC1DAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpRY-Tad-CBE(ACC3)
Plasmid#235082PurposeExpresses SpRY-Tad-CBE(ACC3) in mammalian cells.DepositorInsertTad-CBE(ACC3)-SpRY Cas9 D10A
UseCRISPRTags2xUGI, bpNLS and bpNLSExpressionMammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
lentiMPH v2 (Neo)
Plasmid#92065PurposeModified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).DepositorInsertMS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
UseLentiviralExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable SinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
HLA_A2402
Plasmid#165010PurposeExpression of HLA-A*24:02 cDNADepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSC-NLS-EGFP
Plasmid#221811PurposeLentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)DepositorInsertNLS-EGFP
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#190034Purposevector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13d
ExpressionMammalianMutationA134V,A140V,A141V,A143VPromoterCBh, SV40, hU6Available SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1
Plasmid#37370DepositorAvailable SinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
LV-AR
Plasmid#213819PurposeLenti-viral expression of human androgen receptor wild-type (WT).DepositorInsertAR (human androgen receptor) (AR Human)
UseLentiviralTagsFlag (N terminal on backbone)ExpressionMammalianMutationNo, it is AR wild-type.PromoterEF-1aAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-GRAM-W
Plasmid#211701PurposeExpression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cellsDepositorInsertGRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
TagsEGFPExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCART0016_CROPseq-CAR-Puro_GPC3-BBz
Plasmid#242984PurposeGPC3-BBz CARDepositorInsertGPC3-BBz CAR
UseLentiviralAvailable SinceNov. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
vsv-Sgo1-GFP
Plasmid#108494Purposeexpression of vsv-Sgo1-EGFPDepositorAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hMLKL
Plasmid#208789PurposeInducibly expresses human MLKL, fused to light-activatable Cry2DepositorAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-gate-3xHA-pGK-PUR
Plasmid#107393PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseGateway Cloning and LentiviralTags3xHAExpressionMammalianAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLTS
Plasmid#59386PurposeExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.DepositorTypeEmpty backboneUseGenome engineeringExpressionBacterialAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
NeonGreen-C1-ORP5
Plasmid#220071Purposeoverexpresses ORP5 with a neonGreen fluorophoreDepositorInsertOSBPL5 (OSBPL5 Human)
ExpressionMammalianAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7Available SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) mCherry
Plasmid#176301PurposeExpresses mCherry mRNA from CMV promoterDepositorInsertmCherry
ExpressionMammalianPromoterCMVAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti-ATF4
Plasmid#125238PurposeATF4 expression vectorDepositorAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
HLA_DRB1_04_01
Plasmid#165013PurposeExpression of HLA-DRB1*04:01 cDNADepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTUM4
Plasmid#230905PurposePlasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coliDepositorInsertsExpressionBacterialAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-VSVgSS-15F11-TM
Plasmid#207317PurposeFunctional incorporation of 15F11 scFv (anti-HA) into envelope of virionsDepositorInsert15F11 ScFv
ExpressionMammalianPromoterCMV promoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mGLIS1
Plasmid#33273PurposeRetroviral expression of mouse Glis1DepositorAvailable SinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
CHIKV eGFP-Zeo replicon
Plasmid#249289PurposeSP6-driven CHIKV_LR2006 OPY1 replicon with eGFP-Zeocin reporter selection cassetteDepositorInsertCHIKV LR2006-OPY1 replicon harboring nonstructural genes (nsp1-nsp4) and eGFP-Zeocin ORF in place of structural genes
ExpressionBacterialMutationstructural polyprotein open reading frame deleted…PromoterSP6Available SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only