We narrowed to 12,311 results for: BASE

1. pBAD-SCW

   Plasmid

   #190626

   Purpose

   Bacterial expression of genetically encoded calcium indicator SCW

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   SCW
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMars1CyeGFP

   Plasmid

   #75222

   Purpose

   Mammalian intracellular expression of fluorogen activating protein Mars1Cy-eGFP fusion

   Depositor

   Alan Waggoner

   Article

   Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

   Insert

   Mars1Cy-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pP2AeGFP-Mars1Cy

   Plasmid

   #75223

   Purpose

   Mammalian intracellular expression of fluorogen activating protein Mars1Cy and eGFP

   Depositor

   Alan Waggoner

   Article

   Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

   Insert

   EGFP-p2a-Mars1Cy
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV-hSyn-Lyn-Y-GECO1

   Plasmid

   #58200

   Purpose

   Express the membrane targeted Y-GECO1 in neurons

   Depositor

   Robert Campbell

   Article

   Zhao et al Integr Biol (Camb). 2014 May 19.

   Insert

   Y-GECO1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTorPE-Y-GECO1

   Plasmid

   #55765

   Purpose

   Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Integr Biol (Camb). 2014 May 19.

   Insert

   Y-GECO1.0
   

   Use

   Lab constructed

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBlastR-5xUAS-mCherry-MiniWhite

   Plasmid

   #165911

   Purpose

   Blasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable UAS Response Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Calprotectin S100A9 toehold switch sensor

   Plasmid

   #110717

   Purpose

   Toehold switch sensor to detect Calprotectin S100A9 mRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   Calprotectin S100A9 toehold switch sensor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB-DEST-R1R2-pNeo

   Plasmid

   #141057

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Neomycin resistance gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEos2-Talin-IBS2-7

   Plasmid

   #57424

   Purpose

   Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   Talin IBS2 (Tln1 Mouse)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Talin Integrin Binding Site

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCEP4-001428gp160

    Plasmid

    #123255

    Purpose

    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (001428) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRB20

    Plasmid

    #52559

    Purpose

    serves as PCR-template to generate HR donors for C-terminal YFP-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    eYFP and Blasticigin resistance cassette
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pR6Kg-Omega2

    Plasmid

    #165865

    Purpose

    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101115

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to LPAR1

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    LPAR1-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P315-FBP_10

    Plasmid

    #139701

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and Npga

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC8b-XI3up

    Plasmid

    #176170

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XI-3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMC7-XI3dw

    Plasmid

    #176171

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XI-3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMC8b-XI5up

    Plasmid

    #176172

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XI-5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMC7-XI5dw

    Plasmid

    #176173

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XI-5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMC8b-XII5up

    Plasmid

    #176176

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XII-5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMC7-XII5dw

    Plasmid

    #176177

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XII-5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMC8b-X2up

    Plasmid

    #176162

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site X-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMC7-X2dw

    Plasmid

    #176163

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site X-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1

    Plasmid

    #141025

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTR-L1-PGK-Neo-L2

    Plasmid

    #141007

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Neomycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101113

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to CXCR4

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    CXCR4-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Nano-lantern(cAMP-3.3)/pRSETB

    Plasmid

    #53592

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pJK526

    Plasmid

    #155387

    Purpose

    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK413

    Plasmid

    #155372

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pM215A

    Plasmid

    #173217

    Purpose

    Expresses ScFV-GCN4-sFGFP10M2-GB1

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    ScFV-GCN4-sfGFP10M2-GB1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pM214

    Plasmid

    #173216

    Purpose

    Expresses MCP-mCherry-GFP11-GB1

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    MCP-mCherry-GFP11-GB1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. JBEI-6823

    Plasmid

    #84986

    Purpose

    For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

    Depositor

    Taek Soon Lee

    Article

    George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

    Insert

    MevTaco-PMKco-Mkco
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pC.059

    Plasmid

    #119564

    Purpose

    Level 0 part. Promoter

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    Ptrc10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. LovTAP N538E

    Plasmid

    #61431

    Purpose

    Expresses calmodulin binding protein-LovTAP N538E fusion protein in E. coli cells. LovTAP N358E is a optogenetic protein that can repress the Trp promoter in the presence of blue light.

    Depositor

    Tobin Sosnick

    Article

    Strickland et al Nat Methods. 2010 Aug;7(8):623-6. doi: 1

    Insert

    LovTAP N538E
    

    Use

    Tags

    CBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac operator

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

34. CXCL5 toehold switch sensor

    Plasmid

    #111907

    Purpose

    Toehold switch sensor to detect CXCL5 mRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    CXCL5 toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBGT

    Plasmid

    #62667

    Purpose

    Donor vector for iRFP knock-in via landing pad

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    iRFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENTR-L1-PGK-PuroTK-L2

    Plasmid

    #141005

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    PuroTK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. IG401: pMVP (L1-R5) RIP

    Plasmid

    #121694

    Purpose

    pMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    RIP
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTAL4

    Plasmid

    #31035

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    Tags

    AcV5 and FokI nuclease

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CD4_LD 0_CS G_tTA-BFP

    Plasmid

    #58869

    Purpose

    MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)

    Plasmid

    #70210

    Purpose

    1 Part of 4-part switch

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge