Addgene

We narrowed to 18,950 results for: Cre

Showing: 5451 - 5500 of 18950 results

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pI3-MTU-DO-HIS

Plasmid

#160021

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-URA

Plasmid

#160020

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Uracil selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-LEU

Plasmid

#160006

Purpose

Low-copy expression vector for K. marxianus, leucine selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJet1.2 Tev-Halo tag

Plasmid

#117124

Purpose

C-terminal Tev Halo-Tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Tev-Halo-tag Tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

Tev-Halo-Tag

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-PML peptide

Plasmid

#175246

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

PML (PML Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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mCitrine-USP19 C506S

Plasmid

#78594

Purpose

Express mCitrine-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 C506S (USP19 Human)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
Pdx-PH2/pGL3

Plasmid

#12163

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2002 Dec . 110(12):1839-4

Insert

Pdx1 promoter PH2 domain

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

June 13, 2007

Availability

Academic Institutions and Nonprofits only
lentiCRISPRv2-7SK-Blast

Plasmid

#183620

Purpose

lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

Depositor

Bill Sugden

Article

Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Modified 7SK

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5B-V5

Plasmid

#35931

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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EB3-eGFP-2xC2-iLID

Plasmid

#114419

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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vector-dendra2 PCDNA

Plasmid

#121134

Purpose

mammalian cell expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Wnt7B STOP

Plasmid

#35878

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSec7-Halox2

Plasmid

#115425

Purpose

Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-Halox2

Use

Tags

HaloTag

Expression

Yeast

Mutation

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt7B-V5

Plasmid

#35934

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pUPD-ADH2t (17-21)

Plasmid

#101701

Purpose

ADH2t in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GCTT-[terminator of S. cerevisiae YMR303C (376 bp)]-CGCT

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-PGK1 (01-11)

Plasmid

#101689

Purpose

pPGK1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YCR012W (982bp)]-CCAT

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-vps10-104

Plasmid

#115426

Purpose

Generate the vps10-104 allele via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

vps10-104

Use

Tags

Expression

Yeast

Mutation

domain 1 deletion

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Wnt9A STOP

Plasmid

#35881

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9A (WNT9A Human)

Use

Entry vector

Tags

Expression

Mutation

Silent Mutation A (543) --> G

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-I70V

Plasmid

#47663

Purpose

pAC-EsaR with a208g mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt9A-V5

Plasmid

#35937

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9A (WNT9A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation A (543) --> G

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt6-V5

Plasmid

#35932

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (567) --> A

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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flGalT-SBP-EGFP_puromycin

Plasmid

#65275

Purpose

GalT reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_99a_Citrine

Plasmid

#130589

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_99a

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pME-myristo

Plasmid

#109541

Purpose

Multisite gateway vector for 5' tagging with the 5' myristoylation sequence from ARF5

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

5' myristoylation sequence from ARF5

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-ATG18

Plasmid

#109556

Purpose

Multisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

yeast ATG18 (PLEKHA3)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_381

Plasmid

#179092

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

IDT-Cas9_v2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_157

Plasmid

#179090

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

HypaCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_156

Plasmid

#179089

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-VRER

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_155

Plasmid

#179088

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-VQR

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_154

Plasmid

#179087

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

xCas9-3.7

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_153

Plasmid

#179086

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

evoCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_152

Plasmid

#179085

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

eCas9-1.1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_151

Plasmid

#179084

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-HF

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Wnt8B

Plasmid

#35917

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8B (WNT8B Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pI4-MTU-DO-HIS

Plasmid

#160026

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pI2-MTU-DO-HPH

Plasmid

#160019

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pI2-MTU-DO-LEU

Plasmid

#160017

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX Vav1 Y3F/PH* (W495L) uGFP

Plasmid

#14564

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

PH* (W495L)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
CMVTO-Fyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp

Plasmid

#179668

Purpose

Membrane-bound (Fyn) TEVP-inducible caged-cTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged cTVMVP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX Vav1 Y3F/CR* (C529S) uGFP

Plasmid

#14560

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CR* (C529S)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pTK_TFAP2a_enh_249_Cerulean

Plasmid

#130615

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

TFAP2a_enh_249

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1-p19-T7-EGFPFL

Plasmid

#46310

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
EGFPFL DNA-1
linker
EGFPFL DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pTK_FoxD3_enh_372_Citrine

Plasmid

#130583

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_enh_372

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH013

Plasmid

#42147

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD-G418R (01-21)

Plasmid

#101703

Purpose

G418R in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[G418R (loxP-PAgTEF-kanMX-TAgTEF-loxP)]-CGCT

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Pax7_enh_143_Citrine

Plasmid

#130602

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Pax7_enh_143

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Pax7_enh_194_Cerulean

Plasmid

#130603

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Pax7_enh_194

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Pax7_enh_195_Citrine

Plasmid

#130604

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Pax7_enh_195

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Pax7_enh_199_Citrine

Plasmid

#130605

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Pax7_enh_199

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Pax7_enh_218_Citrine

Plasmid

#130607

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Pax7_enh_218

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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