We narrowed to 11,264 results for: Kars;
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Intron-Tagging-mScarlet-Frame1-Parental-Minicircle
Plasmid#216125PurposeParental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.DepositorInsertsgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
UseCRISPRAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flag-hSox2
Plasmid#20073DepositorAvailable SinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINT2
Plasmid#127512PurposePlasmid encodes H. sapiens codon optimized Integrase 2.DepositorInsertIntegrase 2 coding sequence codon optimized for H. sapiens expression.
ExpressionMammalianAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINT9
Plasmid#127516PurposePlasmid encodes H. sapiens codon optimized Integrase 9.DepositorInsertIntegrase 9 coding sequence codon optimized for H. sapiens expression.
ExpressionMammalianAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINT13
Plasmid#127517PurposePlasmid encodes H. sapiens codon optimized Integrase 13.DepositorInsertIntegrase 13 coding sequence codon optimized for H. sapiens expression.
ExpressionMammalianAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZFP36L2
Plasmid#156149PurposeFor use in RBP tethering screenDepositorAvailable SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
Plasmid#203229PurposeCre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertCre-dependent, membrane-localized Optopatch
UseAAVExpressionMammalianPromoterCAGAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD8-mCherry-FRB
Plasmid#100738PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-C7
Plasmid#194591PurposeBacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertC7 (S )
ExpressionBacterialAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-1(NC)-SH2
Plasmid#46496DepositorInsertSHP-1(NC) (PTPN6 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-221PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSP1101
Plasmid#65773PurposeHuman expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, and T1337R mutations in C…PromoterCMV & T7Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flag-hKlf4
Plasmid#20074DepositorAvailable SinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-Junb
Plasmid#178441Purposedoxycycline-inducible expression of Mouse Junb in mammalian cellsDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Cis1-SH2
Plasmid#46417DepositorInsertCis1 (SOCS1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 90-214PromoterTacAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCK341
Plasmid#192640PurposeExpresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyMutationSoxS has R93A and S101A mutations and dSpRY has s…PromoterBBa_J23107 and Sp.pCas9Available SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs3-SH2
Plasmid#46506DepositorInsertSocs3 (SOCS3 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 22-142PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs1-SH2
Plasmid#46504DepositorInsertSocs1 (SOCS1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-166PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
ESR1-luciferase
Plasmid#113351Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 geneDepositorInsertESR1-enhancer (ESR1 Human)
UseLuciferaseAvailable SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Zap70(NC)-SH2
Plasmid#46535DepositorInsertZap70(NC) (ZAP70 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-268PromoterTacAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-split-intein-Gal4[21-881]-C-int
Plasmid#199204PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-intDepositorInsertT2A-split-intein-Gal4[21-881]-C-int
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-epsin(144-575)-GFP
Plasmid#100733PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertepn1 (Epn1 Mouse)
TagsFKBP and GFPExpressionMammalianMutationclathrin binding middle part aa 144-575PromoterCMVAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-BrCas12b
Plasmid#182276PurposeExpresses recombinant BrCas12bDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis, T7, Thrombin siteExpressionBacterialPromoterT7Available SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD406
Plasmid#163111PurposeExpression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mNeonGreen_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD399
Plasmid#163106PurposeExpression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mEGFP_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
SERPINH1
Plasmid#155999PurposeFor use in RBP tethering screenDepositorAvailable SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRPF19
Plasmid#155847PurposeFor use in RBP tethering screenDepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO PPM1H
Plasmid#207333PurposeBacterial expression of His SUMO PPM1HDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a Wb-iPGM
Plasmid#180246PurposeBacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10DepositorInsertWuchereria bancrofti iPGM-10His
TagsHisExpressionBacterialPromoterT7Available SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
PPIB
Plasmid#155838PurposeFor use in RBP tethering screenDepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b Ag43 160N 6H sfGFP-R5 AmpR
Plasmid#225139PurposeRecombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.DepositorInsertAg43 protein fused to silaffin-R5 and sfGFP
TagsHis tagExpressionBacterialPromoterT7Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flag-cMyc T58A
Plasmid#20075DepositorAvailable SinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
TERT
Plasmid#156078PurposeFor use in RBP tethering screenDepositorAvailable SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET14b His Rab8a Q67L
Plasmid#186014PurposeHis Rab8a Q67LDepositorInsertHis Rab8a Q67L (RAB8A Human)
ExpressionBacterialAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla
Plasmid#174620PurposePiggyBac plasmid expressing EGFP under the control of a cone arrestin promoterDepositorInsertenhanced green fluorescent protein
UsePiggybacExpressionMammalianAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-LivePARBackbone
Plasmid#176526PurposeExpression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePARDepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY003 (pFnCpf1_delta Cas)
Plasmid#69974PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes
UseCRISPRExpressionBacterialMutationCas genes are deleted from locusAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-OB-Linker-eGFP
Plasmid#176068PurposeEGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassetteDepositorInsertOB
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-FLAG.Mm.KAT5
Plasmid#184647PurposeRetroviral expression of mouse Kat5DepositorAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-FLAG.Mm.EPC2
Plasmid#184649PurposeRetroviral expression of mouse EPC2DepositorAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only
