We narrowed to 4,072 results for: p2a

1. SPLICSFAST-LDh-LY-P2A

   Plasmid

   #214895

   Purpose

   Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-LDh-LY-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPLICS Mt-ER- Po Long P2A

   Plasmid

   #164117

   Purpose

   Detect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP Mt-ER- Po Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHDR_sox2HA_P2A-GFP-pA_HA_G2913A

   Plasmid

   #196191

   Purpose

   Tagging human SOX2 gene with P2A-EGFP at the C-terminus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   SOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
   

   Use

   Homology repair donor

   Tags

   Expression

   Mutation

   G2913A mutation to replace the guide cutting site…

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. JDW 1042 (pLenti6.2_CMV_V5_TCF7L1_P2A_H2A_mCherry)

   Plasmid

   #233540

   Purpose

   A CMV driven human TCF7L1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   TCF7L1 (TCF7L1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. JDW 1039 (pLenti6.2_CMV_V5_TCF7_P2A_H2A_mCherry)

   Plasmid

   #233539

   Purpose

   A CMV driven human TCF7 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   TCF7 (TCF7 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. JDW 1045 (pLenti6.2_CMV_V5_TCF7L2_P2A_H2A_mCherry)

   Plasmid

   #233541

   Purpose

   A CMV driven human TCF7L2 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   TCF7L2 (TCF7L2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

   Plasmid

   #233538

   Purpose

   A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_Ef1a:PE2-P2A-GFP

   Plasmid

   #184445

   Purpose

   Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

   Depositor

   Bobby Koeleman

   Article

   Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Ef1A

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTACR TH1-p2aGFP

   Plasmid

   #135815

   Purpose

   CRISPR based on px330 p2aGFP targeting TH gene near stop codon

   Depositor

   Lee Rubin, Tim Ahfeldt

   Article

   Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

   Insert

   TH 1 guide
   

   Use

   CRISPR

   Tags

   p2a Myr-eGFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTACR TH2-p2aGFP

    Plasmid

    #135816

    Purpose

    CRISPR based on px330 p2aGFP targeting TH gene near stop codon

    Depositor

    Lee Rubin, Tim Ahfeldt

    Article

    Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

    Insert

    TH 2 guide
    

    Use

    CRISPR

    Tags

    p2a Myr-eGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ALCAM-p2A-E2Crimson

    Plasmid

    #83939

    Purpose

    Lentiviral vector expressing ALCAM-p2A-E2Crimson

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    ALCAM (ALCAM Human)
    

    Use

    Lentiviral

    Tags

    p2A-E2Crimson

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. scFLARE1-p2A-eGFP

    Plasmid

    #158699

    Purpose

    Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    scFLARE1-p2A-eGFP
    

    Use

    AAV

    Tags

    Flag, V5. eGFP

    Expression

    Mammalian

    Mutation

    Truncated form of TEV protease (220-242 amino aci…

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry

    Plasmid

    #85436

    Purpose

    Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Pembrolizumab and anti-CTLA4 scFv
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pHR_TetO_CD19-P2A-mCherry

    Plasmid

    #133803

    Purpose

    repressible expression of CD19 and mCherry in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2018 Jul 13;361(6398):156-162.

    Insert

    putative ECD of H. sapiens CD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_rtTA-P2A-BFP_HygroR

    Plasmid

    #186738

    Purpose

    rtTA-P2A-BFP expression construct cloned into lentiviral backbone

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    rtTA
    

    Use

    Lentiviral

    Tags

    P2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPLICS Po-Mt Short P2A

    Plasmid

    #164116

    Purpose

    Detect the short Peroxisomes-mitochondria contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1+ Flag-RAP2A

    Plasmid

    #128099

    Purpose

    Expresses RAP2A in mammalian cells in a G418-selectable plasmid

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid

    #189755

    Purpose

    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid

    #189752

    Purpose

    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mTurquoise2_spatial-linker-P2A_mScarlet3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTransposon.CAG.CasRX-P2A-Blast

    Plasmid

    #212965

    Purpose

    Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    CasRx
    

    Use

    Transposon

    Tags

    P2A-Blasticidin resistance

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLNCX2-mCherry-CHMP2A

    Plasmid

    #115330

    Purpose

    expresses mCherry-CHMP2A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMK312 (TOP2A CRISPR)

    Plasmid

    #140654

    Purpose

    TOP2A tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LV_EF1a_CCNB1-P2A-Hygro_Barcode

    Plasmid

    #170218

    Purpose

    Barcoded lentiviral vector to express CCNB1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CCNB1 (CCNB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFH2.9_MMLV_Gag-P2A-eGFP

    Plasmid

    #205524

    Purpose

    Express MMLV Gag-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AmCyan-P2A-mCherry

    Plasmid

    #45350

    Depositor

    Ilpo Huhtaniemi

    Article

    Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

    Insert

    AmCyan
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    SV40 NLS

    Promoter

    CMV

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pH1R-P2A-mCherry-N1

    Plasmid

    #84330

    Purpose

    Co-expresses (untagged) Histamine-1 receptor and mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H1R-P2A-mCherry (HRH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SPLICS PM-ER Short P2A

    Plasmid

    #164112

    Purpose

    Detect the short Plasma membrane-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP PM-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR_TRE_Ncad-P2A-GFPlig

    Plasmid

    #133806

    Purpose

    inducible expression of Ncad and membrane-tethered GFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2018 Jul 13;361(6398):156-162.

    Insert

    cadherin 2 (CDH2) (Cdh2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB-Aka-P2A-GFP

    Plasmid

    #120867

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Akaluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    insert Akaluc and a P2A site

    Promoter

    CAG Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet

    Plasmid

    #130509

    Purpose

    expresses mScarlet

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge