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We narrowed to 34,443 results for: CaS

Showing: 5951 - 6000 of 34443 results

  9. pJK430

    Plasmid
    #155377
    Purpose
    PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A2NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    155377-plasmid-map-sequence-id-309006

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  10. pJK520

    Plasmid
    #155386
    Purpose
    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    155386-plasmid-map-sequence-id-309003

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  11. pAIP4_6BIO

    Plasmid
    #234152
    Purpose
    Heterologous protein expression of clan CA, family C40, NlpC/P60 superfamily cysteine peptidase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6BIO (TVAG_298080 Trichomonas vaginalis)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234152-plasmid-map-sequence-id-464118

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  15. pCAT009

    Plasmid
    #171635
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171635-plasmid-map-sequence-id-336490

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  16. pB025

    Plasmid
    #183092
    Purpose
    Expresses FnCas12a in Bacteroides and used for genome editing
    Depositor
    Lei Dai
    Article
    Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47
    Insert
    FnCas12a, gRNA, Promoter, HAB, TetR,
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDPGH023
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183092-plasmid-map-sequence-id-368147

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  17. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60621-plasmid-map-sequence-id-208224

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  18. DR274

    Plasmid
    #42250
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription
    Depositor
    Keith Joung
    Article
    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR; Zebrafish expression
    Tags
    Expression
    Worm
    Mutation
    Promoter
    T7
    Available since
    Jan. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    42250-plasmid-map-sequence-id-254852

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  28. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  29. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1-a
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  31. pCAT005

    Plasmid
    #171632
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171632-plasmid-map-sequence-id-336487

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  32. pMT448

    Plasmid
    #139388
    Purpose
    Bile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1),
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    Jan. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    139388-plasmid-map-sequence-id-313834

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  33. pMT450

    Plasmid
    #139390
    Purpose
    Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    Jan. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    139390-plasmid-map-sequence-id-313840

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  34. pMT492

    Plasmid
    #139397
    Purpose
    Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    Jan. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    139397-plasmid-map-sequence-id-313938

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  36. pMT493

    Plasmid
    #139398
    Purpose
    Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139398-plasmid-map-sequence-id-313939

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  37. pMT494

    Plasmid
    #139399
    Purpose
    Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139399-plasmid-map-sequence-id-313941

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  38. pMT449

    Plasmid
    #139389
    Purpose
    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139389-plasmid-map-sequence-id-313839

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  39. pMT451

    Plasmid
    #139391
    Purpose
    Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    BA inducible promoters
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139391-plasmid-map-sequence-id-313926

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  40. pMT455

    Plasmid
    #139392
    Purpose
    aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    aTC and BA inducible promoters
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139392-plasmid-map-sequence-id-313930

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  43. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

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  44. pCAT007

    Plasmid
    #171634
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171634-plasmid-map-sequence-id-336493

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  45. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Kam Leong
    Article
    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Promoter
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62195-plasmid-map-sequence-id-101643

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  46. pTU422

    Plasmid
    #178982
    Purpose
    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)
    Depositor
    Stan Brouns
    Article
    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353
    Inserts
    gRAMP
    CRISPR array
    Use
    Tags
    Strep-Tag II SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178982-plasmid-map-sequence-id-354261

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  47. pDuRCC-N

    Plasmid
    #81194
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please view depositor comments below and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81194-plasmid-map-sequence-id-160426

    Enlarge

  49. pJRH056

    Plasmid
    #171637
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-tdTomato
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171637-plasmid-map-sequence-id-354439

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Showing: 5951 - 6000 of 34443 results