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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 10,599 results for: cat.1

Showing: 4841 - 4880 of 10599 results

  4. GB1531

    Plasmid
    #215242
    Purpose
    TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized.
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    PNos:PhiC31:TNos
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    215242-plasmid-map-sequence-id-436558

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  5. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

    Plasmid
    #201534
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    rhaS W190G
    Promoter
    PrhaBAD (rhamnose)
    Available since
    Sept. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201534-plasmid-map-sequence-id-406801

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  7. pZA16

    Plasmid
    #158494
    Purpose
    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codon
    Depositor
    Sophien Kamoun
    Article
    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.
    Insert
    ZAR1
    Use
    Golden gate entry vector
    Tags
    Expression
    Mutation
    L17E, D481V, no STOP codon
    Promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    158494-plasmid-map-sequence-id-309733

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  10. pTon1(acgfp1)

    Plasmid
    #233648
    Purpose
    Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Takeo Kubo
    Article
    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10
    Insert
    target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233648-plasmid-map-sequence-id-468211

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  11. pTDpelB-C_sfYFPTwinStrep

    Plasmid
    #45944
    Purpose
    expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Insert
    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    Use
    Tags
    PelB export sequence and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq/Ptrc
    Available since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45944-plasmid-map-sequence-id-64657

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  12. pTDpelB-NTwinStrep

    Plasmid
    #45940
    Purpose
    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Type
    Empty backbone
    Use
    Tags
    6xHis, PelB, and Twin-Strep
    Expression
    Bacterial
    Mutation
    Promoter
    Trc (lactose/IPTG inducible)
    Available since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45940-plasmid-map-sequence-id-64653

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  17. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid
    #201533
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    rhaR L128+1bp (frameshift), ilvA G455C
    Promoter
    PrhaBAD (rhamnose)
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201533-plasmid-map-sequence-id-398133

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  18. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

    Plasmid
    #201535
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    rhaS ΔM1-H5, PrhaSR Δ179bp
    Promoter
    PrhaBAD (rhamnose)
    Available since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201535-plasmid-map-sequence-id-398128

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  20. pCMV-TALEN-Rep

    Plasmid
    #45964
    Depositor
    Ralf Kuehn
    Insert
    beta-Galactosidase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    duplicated aa 1-135
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    45964-plasmid-map-sequence-id-64559

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  21. pCMV-Rab-Rep

    Plasmid
    #45967
    Depositor
    Ralf Kuehn
    Insert
    beta-Galactosidase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    duplicated aa 1-135
    Promoter
    CMV
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    45967-plasmid-map-sequence-id-64561

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  26. Plas-gRNA-Z3EV

    Plasmid
    #157659
    Purpose
    GFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.
    Depositor
    Frank Albert
    Article
    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1
    Insert
    GFP-gRNA NatMX Z3EV
    Use
    Insert storage (replicative in e. coli)
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    157659-plasmid-map-sequence-id-309102

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  27. pET28a-shortSUMO1

    Plasmid
    #228951
    Purpose
    express a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimized
    Depositor
    Marcin Suskiewicz
    Insert
    SUMO1 (SUMO1 Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-17
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228951-plasmid-map-sequence-id-472406

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  33. TRIN-shREN

    Plasmid
    #105586
    Purpose
    Dox-inducible mir30 shREN (control shRNA)/dsRED expression with Venus marker and Neo resistance
    Depositor
    Christopher Vakoc
    Article
    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.
    Insert
    control shRNA targeting luciferase
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 4841 - 4880 of 10599 results