Skip to main content
Addgene
Showing: 4921 - 4960 of 6325 results

  1. pCMV(MinDis)-iCyt_BrEt_SnFR_PM

    Plasmid
    #177747
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    pCMV(MinDis)-iCyt_BrEt_SnFR_PM
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177747-plasmid-map-sequence-id-349063

    Enlarge

  2. _pCMV(MinDis)-iCyt_F_SnFR_PM

    Plasmid
    #177745
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    _pCMV(MinDis)-iCyt_F_SnFR_PM
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177745-plasmid-map-sequence-id-349553

    Enlarge

  4. pCMV(MinDis)-iCyt_BrEt_SnFR_ER

    Plasmid
    #177746
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    pCMV(MinDis)-iCyt_BrEt_SnFR_ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177746-plasmid-map-sequence-id-349555

    Enlarge

  5. _pCMV(MinDis)-iCyt_F_SnFR_ER

    Plasmid
    #177744
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    _pCMV(MinDis)-iCyt_F_SnFR_ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177744-plasmid-map-sequence-id-349548

    Enlarge

  14. pFUSEss-CHIg-mG3_CH2charge

    Plasmid
    #105858
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG3 heavy chain (Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutated heavy chain of mouse IgG3, muatations: Hi…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    105858-plasmid-map-sequence-id-206030

    Enlarge

  15. pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500

    Plasmid
    #92315
    Purpose
    Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    B1 domain of protein L (PL) mutant K16TAG
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    p70b (p70a promoter with one mutation)
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    92315-plasmid-map-sequence-id-197049

    Enlarge

  24. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

    Enlarge

  28. pCasper4-g-osk-TRICK

    Plasmid
    #71244
    Purpose
    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.
    Depositor
    Anne Ephrussi
    Article
    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.
    Insert
    oskar (osk Fly)
    Use
    P element-mediated germline transformation
    Tags
    12xPP7 and 6xMS2
    Expression
    Insect
    Mutation
    Promoter
    osk
    Available since
    Dec. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    71244-plasmid-map-sequence-id-127267

    Enlarge

  30. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

    Plasmid
    #188072
    Purpose
    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.
    Depositor
    Kristen Verhey
    Article
    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e
    Insert
    rat KIF5C (Kif5c Rat)
    Use
    Tags
    Flag and Halo
    Expression
    Mammalian
    Mutation
    aa1-560, deletion of aa2-6
    Promoter
    CMV
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    188072-plasmid-map-sequence-id-368400

    Enlarge

  34. pLenti-PalmGRET

    Plasmid
    #158221
    Purpose
    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.
    Depositor
    Charles P. Lai
    Article
    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014
    Insert
    PalmGFP-Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    158221-plasmid-map-sequence-id-309022

    Enlarge

Showing: 4921 - 4960 of 6325 results