We narrowed to 7,249 results for: Cit

1. pAG416-Gal-PA50

   Plasmid

   #84902

   Purpose

   induce expression of dipeptide repeat PA in yeast

   Depositor

   Aaron Gitler

   Article

   Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

   Insert

   PA50 (C9orf72 Synthetic)
   

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG416-Gal-GA50

   Plasmid

   #84903

   Purpose

   induce expression of dipeptide repeat GA in yeast

   Depositor

   Aaron Gitler

   Article

   Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

   Insert

   GA50 (C9orf72 Synthetic)
   

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

   Plasmid

   #65455

   Purpose

   Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSPL3-hRPH3A_c.444Gwt

   Plasmid

   #190476

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGB3 omega1 P35S:MS2:VPR:Tnos - P35S:dCas9:EDLL:Tnos (GB2085)

   Plasmid

   #160645

   Purpose

   Module for the expression of Ms2 protein fused to VPR and dCas9 fused to EDLL

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35s-Ms2:VPR-Tnos-35s-dCas9:EDLL-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsmBI sites removed

   Promoter

   35S x2

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PDGFRalpha-D842V #1

   Plasmid

   #201407

   Purpose

   Inserts PDGFRalpha-D842V to activate the MAP kinase pathway.

   Depositor

   Maria Castro

   Article

   Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

   Insert

   PDGFRalpha-D842V (Pdgfra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D842V

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo

   Plasmid

   #222636

   Purpose

   Ezrin activation reporter

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   miRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1 KBTBD4 WT

   Plasmid

   #184624

   Purpose

   Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells

   Depositor

   Vincenzo D'Angiolella

   Article

   Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

   Insert

   N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   no mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCerulean3-Mcl-1-pLVX

   Plasmid

   #166752

   Purpose

   Express mCerulean3 fused to the N-terminus of Bcl-2 family protein, Mcl-1, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Mcl-1 (MCL1 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-α-Syn-nYFP

    Plasmid

    #92203

    Purpose

    Retroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    SNCA (SNCA Human)
    

    Use

    Retroviral

    Tags

    YFP(1-155)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 61426-hU6-shmFmr1-EF1a-mCherry

    Plasmid

    #157859

    Purpose

    Expresses a shRNA targeting mouse Fmr1 gene

    Depositor

    Xinyu Zhao

    Article

    Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

    Insert

    Fmr1 (Fmr1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

    Plasmid

    #122102

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)

    Plasmid

    #200977

    Purpose

    Mammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    D62Q-Q130L-V167L (increase specificity towards he…

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

    Plasmid

    #200980

    Purpose

    Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Use

    Tags

    IgG1 Fc

    Expression

    Mammalian

    Mutation

    Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSPL3-hRPH3A_c.1853Gmut

    Plasmid

    #190479

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.1853A>G

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42

    Plasmid

    #165436

    Purpose

    Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
    

    Use

    Tags

    ShadowY, Clover(T153M/F223R)

    Expression

    Mammalian

    Mutation

    H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

    Promoter

    CMV

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX LYCHOS (GPR155) WT-FLAG

    Plasmid

    #199633

    Purpose

    Lentiviral construct to stably express LYCHOS (GPR155) WT

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    GPR155 (GPR155 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX4T2-Ste7-EE

    Plasmid

    #52681

    Purpose

    expression of constitutively active Ste7 kinase in bacteria

    Depositor

    Benjamin Turk

    Article

    Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

    Insert

    Ste7 (STE7 Budding Yeast)
    

    Use

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    S359E/T363E constitutively active

    Promoter

    tac

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

19. AAV-PGK-Chst3

    Plasmid

    #193024

    Purpose

    AAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoter

    Depositor

    Bart Nieuwenhuis

    Article

    Yang et al Mol Psychiatry. 2021 Jul 16. pii: 10.103

    Insert

    Chst3 (Chst3 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Pvalb-2A-Cre targeting vector

    Plasmid

    #61570

    Purpose

    Target the Cre recombinase gene to the stop codon of the mouse Pvalb gene

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Pvalb exon 4 - 2A - Cre
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. Pvalb-2A-Flpo Flp-in replacement vector

    Plasmid

    #61572

    Purpose

    Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Pvalb-2A-Flpo
    

    Use

    Recombinase-mediated cassette exchange using flp …

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid

    #160621

    Purpose

    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    U6-26:gRNA 4 (pNos): 2.1 aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. Snap25-LSL-2A-GFP targeting vector

    Plasmid

    #61575

    Purpose

    Target a Cre-dependent GFP expression cassette to the stop codon of the mouse Snap25 gene

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Snap25-LSL-2A-GFP
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSN25

    Plasmid

    #100110

    Purpose

    expresses a chimeric SOX2-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to full length HP1 alpha (SOX2, CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA

    Plasmid

    #65453

    Purpose

    Can be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV1

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetO

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PX458-3xHA-SpCas9

    Plasmid

    #130968

    Purpose

    3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    3xHA-NLS-SpCas9
    

    Use

    CRISPR

    Tags

    3x HA, EGFP, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMG023 MBP-Axin 384-518-His (miniAxin)

    Plasmid

    #154012

    Purpose

    Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    381-518 fragment

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRK5-LYCHOS (GPR155) WT-myc

    Plasmid

    #199684

    Purpose

    Transient expression of LYCHOS (GPR155) WT

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    GPR155 (GPR155 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRK5-LYCHOS (GPR155) 4CA-HA

    Plasmid

    #199677

    Purpose

    Transient expression of LYCHOS (GPR155) C595.604.629.638A mutant

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    GPR155 (GPR155 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C595.604.629.638 mutated to A

    Promoter

    CMV

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX LYCHOS-FLAG-TiD

    Plasmid

    #199747

    Purpose

    Lentiviral construct to stably express LYCHOS (GPR155) TurboID

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    LYCHOS (GPR155 Human)
    

    Use

    Tags

    Flag and TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge