We narrowed to 9,307 results for: control

1. pMT[Hygro]_GBP-3xFlag-DEST

   Plasmid

   #157813

   Purpose

   A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection

   Depositor

   Zoltan Lipinszki

   Article

   Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:

   Insert

   GFP-binding protein
   

   Use

   For drosophila cells

   Tags

   3xFlag

   Expression

   Insect

   Promoter

   metallothionein

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LightR-bRaf-mVenus

   Plasmid

   #162154

   Purpose

   Expresses LightR-bRaf-mVenus in mammalian cells

   Depositor

   Andrei Karginov

   Article

   Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

   Insert

   LightR-bRaf-mVenus
   

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Valine 600 is substituted with Glutamic acid

   Promoter

   CMV

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-NLC-DHFR-SpCas9

   Plasmid

   #124523

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NLC-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMXs-hs-3xHA-delta6xNHL-LIN-41

   Plasmid

   #52722

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 593–868

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

   Plasmid

   #69587

   Purpose

   Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53-L344P (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - C term

   Expression

   Mammalian

   Mutation

   p53 L344P mutation that prevents dimerization and…

   Promoter

   EF1alpha

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMSCV-Flag-Zfp423-deltaSBD

   Plasmid

   #35971

   Depositor

   Bruce Spiegelman

   Article

   Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

   Insert

   Zinc Finger Protein 423 (Zfp423 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   lacks zinc fingers 14-19 (aa 640 -838) (delta SMA…

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

7. INCTbiosyn-pAct-AtINTBxb1

   Plasmid

   #127536

   Purpose

   Plasmid encodes A. thaliana codon optimized Integrase Bxb1.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.
   

   Expression

   Plant

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBMN-puro-P2A-FRB-Fis1

   Plasmid

   #135297

   Purpose

   stably express FBR-Fis1 in mammalian cells with puromycin selection

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   FRB-Fis1 (FIS1 Human)
   

   Use

   Retroviral

   Tags

   FRB

   Expression

   Mammalian

   Mutation

   Fis1 tail (aa 93-152)

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-HA-RIPK1 K115R

   Plasmid

   #78835

   Purpose

   To overexpress RIPK1 K115R in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K115R

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

    Plasmid

    #51255

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Ala

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

    Plasmid

    #22018

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pDest27-mBIN1+17

    Plasmid

    #61096

    Purpose

    Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

    Depositor

    Robin Shaw

    Article

    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

    Insert

    mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLJM1 Flag GFP 4E-BP1 Rheb15

    Plasmid

    #112761

    Purpose

    express GFP-4EBP1-Rheb15

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Rheb15

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTH726-CEN-RLuc/minCFLuc

    Plasmid

    #38210

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAFNF-PSDd1.2-GFP

    Plasmid

    #125581

    Purpose

    Flp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    PSDd1.2-GFP (Dlg4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT-T0-Flag-HROB

    Plasmid

    #135298

    Purpose

    Expresses Flag-tagged HROB in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Hustedt et al Genes Dev. 2019 Oct 1;33(19-20):1397-141

    Insert

    HROB (HROB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pMXs-hs-3xHA-deltaRING-LIN-41

    Plasmid

    #52718

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 12-91

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBabe-TetCMV-puro-mCerulean-PA-Rac1

    Plasmid

    #22031

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pBabe-PI(WT)-Rac1(WT)-mCherry

    Plasmid

    #87550

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive Rac1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Rac1 (RAC1 Human)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe-PI(WT)-Rac1(Q61L)-mCherry

    Plasmid

    #87553

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Rac1 (RAC1 Human)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Q61L

    Promoter

    CMV

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

    Plasmid

    #22032

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mPA-GFP-PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

22. PA-DreN

    Plasmid

    #169707

    Purpose

    The optimized DreN246-nMag

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PA-DreC

    Plasmid

    #169705

    Purpose

    The optimized pMag-Dre247

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    pMag-Dre247
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. J23117-mRFP1-331Bb

    Plasmid

    #78280

    Purpose

    mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23117-mRFP1
    

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

    Plasmid

    #51256

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Glu

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

26. His6tev CBP BCHZDeltaAL

    Plasmid

    #99551

    Purpose

    Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

    Depositor

    Peter Wright

    Article

    Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

    Insert

    mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)
    

    Tags

    His6tev

    Expression

    Bacterial

    Mutation

    replaced 1557-1618 with SGGSG

    Promoter

    T7

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA HA-Gnat1 WT-3UTR

    Plasmid

    #184026

    Purpose

    Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Gnat1 (Gnat1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOTTC1619 pAAV SYN1 Nuc-EYFP miR-30 SST

    Plasmid

    #135565

    Purpose

    An AAV vector expressing miR-30a shRNA vs rat SOM (aka SST) and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    miR-30 rat SST
    Nuc-EYFP
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    SYN1 and hSYN1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAVS1 ObLiGaRe Donor vector/EPB58

    Plasmid

    #90016

    Purpose

    Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

    Depositor

    Eric-Paul Bennett

    Article

    Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

    Insert

    MCS flanked by inverted ZFN binding sites (PPP1R12C Human)
    

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

    Plasmid

    #107730

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

    Plasmid

    #107729

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-HA-RIPK3 2KR(55,363)

    Plasmid

    #78811

    Purpose

    To overexpress RIPK3 2KR(55,363) in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K55R, K363R

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEF5/FRT/DEST-3xHA/mGli2 P1-4A

    Plasmid

    #51252

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 789,805,817,848 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

34. p3F-Strep-hMIB2

    Plasmid

    #33313

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    MIB2 (MIB2 Human)
    

    Tags

    3xFlag and StrepOne

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

35. INCTbiosyn-pAct-AtINT4

    Plasmid

    #127530

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase 4.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 4 coding sequence codon optimized for A. thaliana expression.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOTTC1618 pAAV SYN1 Nuc-EYFP miR-30 FF3

    Plasmid

    #135564

    Purpose

    An AAV vector expressing miR-30a shRNA vs FF luciferase and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    miR-30 FF3
    Nuc-EYFP
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    SYN1 and hSYN1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-FLAG-RIPK1 K163R

    Plasmid

    #78844

    Purpose

    To overexpress RIPK1 K163R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K163R

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid

    #133085

    Purpose

    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    Crz1*-CLASP
    

    Expression

    Bacterial and Yeast

    Promoter

    pADH1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. J23114-mRFP1-331Bb

    Plasmid

    #78279

    Purpose

    mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23114-mRFP1
    

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-ULK2-K39I

    Plasmid

    #97076

    Purpose

    Expresses mutant ULK2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    ULK2 (ULK2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 39 to Isoleucine

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge