We narrowed to 18,759 results for: nls
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Plasmid#171385PurposeExpression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized minidCas13X.1-RESCUE-S
ExpressionMammalianPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
Plasmid#162037PurposeBackground-free mRNA labeling tripartite superfolder GFP systemDepositorInsertGFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
UseLentiviralExpressionMammalianAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1
Plasmid#138417PurposeCAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1DepositorInsertsBirA
dCas9
UseLentiviralTagsFLAG, Avi-tag and V5, HisPromoterEF1aAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-BE4max-NRCH
Plasmid#136920PurposeExpresses BE4max-NRCH in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE4max-NRCH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Grin2b KI
Plasmid#131487PurposeEndogenous tagging of GluN2b: N-terminal (amino acid position: S34)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA and GFP donor (Grin2b Rat)
ExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORANGE GFP-Grin1 KI
Plasmid#131485PurposeEndogenous tagging of GluN1: N-terminal (amino acid position: A20)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SaABEmax
Plasmid#119814PurposeA-to-G base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertecTadA-ecTadA*(7.10)-nSaCas9
ExpressionMammalianAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin1
Plasmid#124852PurposeMutagenesis of Grin1DepositorInsertGrin1 (Grin1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA
Plasmid#107311PurposeEncoding scFv-p65-HSF1 driven by EF1α promoterDepositorInsertEF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA
UseLentiviralExpressionMammalianAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330_human CDK1
Plasmid#118597PurposeOne shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertguide RNA against human CDK1
UseCRISPRAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti_SaCRISPR_GFP
Plasmid#118636PurposeTo clone sgRNA for SaCas9 (SauCas9)DepositorTypeEmpty backboneUseLentiviralPromoterU6Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-CMV-TetO2_3C-Twin-Strep
Plasmid#113883PurposeMammalian (inducible) protein expressionDepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepPromoterCMV-MIE-TetO2Available sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV_AncBE4max_3xHA
Plasmid#112097PurposeC:G-to-T:A base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAncBE4max_3xHA
UseCRISPRTags3xHAExpressionMammalianPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1a-Archon1-KGC-EGFP-ER2
Plasmid#108425PurposeLentivirus production plasmid encoding for Archon1 fluorescent voltage reporterDepositorInsertArchon1-KGC-EGFP-ER2
UseLentiviralExpressionMammalianPromoterEF1aAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4Cre
Plasmid#98039PurposeSecond-generation rabies viral vector genomeDepositorInsertmCre
ExpressionMammalianPromoterCMVAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJH2972
Plasmid#100956PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX markerDepositorPublicationTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
bRA89
Plasmid#100950PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-dCas9-dMQ1-EGFP
Plasmid#89637PurposeTargeted CpG methylationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG, 6*His, and T2A-EGFPExpressionMammalianMutationC141S, TGC-->TCC; S317A, AGC-->GCCPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHSE901
Plasmid#91708PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygRDepositorTypeEmpty backboneExpressionPlantAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGH183_MS2-AIDΔDead-Hygro
Plasmid#85414Purposelentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable markerDepositorInsertMS2-AIDΔDead and Hygromycin resistance
UseCRISPR and LentiviralExpressionMammalianAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA1-gRNA_A
Plasmid#74374PurposegRNA_A to knockout human AMPK alpha 1 using Cas9nDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
UseCRISPRPromoterU6Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ167
Plasmid#69309PurposeGateway entry vector with Cas9pDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9p (Plant codon-optimized; high GC content at 5 prime region)
UseCRISPRMutationNLS fusionAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70
Plasmid#62947PurposeCreating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertT2A-LexA::QFAD-Hsp70
Available sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330S-7
Plasmid#58783PurposeExpresses Cas9 nuclease and gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60227PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianPromoterCBh and U6Available sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.PAC
Plasmid#58329PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter drivenDepositorAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-OC-IRES-BSD
Plasmid#53118PurposeTo create stable cell clones with high-level expression of Cas9 and OCT1DepositorInsertsUseLentiviralTagsIRES-BSD, NLS, and P2AExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTALYM4
Plasmid#47875PurposeDestination plasmid with mRuby2 for Golden Gate reaction of TALE assemblyDepositorTypeEmpty backboneUseTALENTagsmRuby2ExpressionMammalianPromoterCAGAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL1180-HR-PUbECFP
Plasmid#47917PurposeDonor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.DepositorInsertECFP
TagsNLSExpressionInsectPromoterAedes aegypti polyubiquitin (PUb)Available sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lox-Stop-Lox TOPO Hygro
Plasmid#35688DepositorInsertloxP-STOP-loxP
UseCre/LoxExpressionMammalianAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
JDS70
Plasmid#32285DepositorTypeEmpty backboneUseT7Tags3X Flag and FOKI WTExpressionMammalianPromoterCMVAvailable sinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHS1517
Plasmid#205980PurposeFLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas5-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1862
Plasmid#205975PurposeFLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas5 from JAGBLH010000220, Cas8-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1859
Plasmid#205972PurposeFLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCsy3/Cas7 from JAGBLH010000220, Cas8-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1860
Plasmid#205973PurposeFLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas6/Csy4 from JAGBLH010000220, Cas8-HNH
TagsFLAG-SV40 NLSExpressionMammalianPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version2
Plasmid#197932PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTags2xNLS-2xFLAGExpressionPlantPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
INS-GFP-CD19-EPT
Plasmid#210469PurposeDonor plasmid for knock-in GFP-CD19 into the human INS locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertINS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
UseCRISPRAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK435
Plasmid#236573PurposeCloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_BDepositorTypeEmpty backboneExpressionPlantAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
Plasmid#207180PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a PromoterDepositorPublicationInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV enAsCas12f-YHAM-sgRNA_v7
Plasmid#208356PurposeExpression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertenAsCas12f-YHAM
ExpressionMammalianMutationF48Y/S188H/V232A/E316MPromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only