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pU6-(BbsI)_CBh-Cas9-T2A-mCherry

Plasmid

#64324

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry

Expression

Mammalian

Promoter

CBh and U6

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

119 Articles

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pAAV-CAG-GFP

Plasmid

#37825

Purpose

AAV-mediated expression of GFP under the CAG promoter

Depositor

Edward Boyden

Has service

AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, AAV9-X1.1, AAV11, AAV11 trial size, AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, and AAV Retrograde trial size

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

172 Articles

1 Data Hub Report

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pLTR-RD114A

Plasmid

#17576

Depositor

Jakob Reiser

Publication

Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Insert

RD114 envelope glycoprotein

Use

Lentiviral

Expression

Mammalian

Available since

April 14, 2008

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

16 Articles

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pCXWB-EBNA1

Plasmid

#37624

Purpose

Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

Depositor

Shinya Yamanaka

Publication

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Expression

Mammalian

Promoter

CAG

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

73 Articles

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pmiRFP670-N1

Plasmid

#79987

Purpose

Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miRFP670

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pSico

Plasmid

#11578

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Publication

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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Lentiviral-TOP-dGFP-reporter

Plasmid

#14715

Purpose

Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

Depositor

Tannishtha Reya

Publication

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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HA GSK3 beta S9A pcDNA3

Plasmid

#14754

Purpose

Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

Depositor

Jim Woodgett

Publication

Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

Insert

GSK3 beta S9A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A. Constitutively active mutant.

Available since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

68 Articles

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myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Publication

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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pCMV-ABE7.10

Plasmid

#102919

Purpose

expresses ABE7.10 in mammalian cells

Depositor

David Liu

Publication

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE7.10

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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pRK5-HA-Ubiquitin-K48R

Plasmid

#17604

Purpose

Mammalian expression of HA tagged ubiquitin K48R

Depositor

Ted Dawson

Publication

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48R (expected to disrupt G76-K48 ubiquitin chain…

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

66 Articles

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pLenti CMV GFP Hygro (656-4)

Plasmid

#17446

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has service

Lentiviral Prep

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

62 Articles

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pY016 (pcDNA3.1-hLbCpf1)

Plasmid

#69988

Purpose

Expresses humanized LbCpf1

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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V5-miniTurbo-NES_pCDNA3

Plasmid

#107170

Purpose

expresses V5-tagged miniTurbo in the mammalian cytosol

Depositor

Alice Ting

Publication

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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Ai9

Plasmid

#22799

Purpose

Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

Depositor

Hongkui Zeng

Publication

Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Insert

CAG-Floxed tdTomato

Use

Mouse Targeting

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

48 Articles

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pSLQ1658-dCas9-EGFP

Plasmid

#51023

Purpose

Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

Depositor

Bo Huang, Stanley Qi

Publication

Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

Insert

dCas9 fuse to EGFP

Use

CRISPR and Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

MSCV LTR promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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EGFP-alphasynuclein-WT

Plasmid

#40822

Depositor

David Rubinsztein

Publication

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNCA (SNCA Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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1036 pcDNA3 Myr HA Akt1

Plasmid

#9008

Purpose

Mammalian expression of human Akt1 with myr, HA tags

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

77 Articles

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pLV-CMV-LoxP-DsRed-LoxP-eGFP

Plasmid

#65726

Purpose

Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

Depositor

Jacco van Rheenen

Publication

Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

Insert

LoxP-DsRed-LoxP-eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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