166,224 results

1. mCherry-FKBP-MTM1

   Plasmid

   #51614

   Purpose

   Recruitable PtdIns3P/PtdIns(3,5)P2 phosphatase

   Depositor

   Tamas Balla

   Article

   Hammond et al J Cell Biol. 2014 Apr 7.

   Inserts

   FKBP
   MTM1 (MTM1 1812, Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

   Plasmid

   #216244

   Purpose

   Express AtAFB2.F74A mutant with mCherry tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   AtAFB2.F74A-mCherry
   

   Use

   CRISPR, TALEN, and Unspecified ; Human safe harbo…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F74A mutation

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAD-sfGFP(Sp)-Pman

   Plasmid

   #133833

   Purpose

   Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(Sp)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Expression

   Bacterial

   Promoter

   Pman

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

   Plasmid

   #35152

   Depositor

   Jay Keasling, Taek Soon Lee

   Article

   Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

   Insert

   AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   R364S in Mevalonate Kinase

   Promoter

   LacUV5, pTrc

   Available Since

   May 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBRPB CAG-mCherry-IP

   Plasmid

   #106333

   Purpose

   PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   mCherry-IRES-Puro
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1477 pcDNA3 flag HA Akt1

   Plasmid

   #9021

   Depositor

   William Sellers

   Article

   Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   HA and flag

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-miRFP670

   Plasmid

   #91854

   Purpose

   Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

   Depositor

   Ralf Kuehn

   Inserts

   hSpCas9-2A-miRFP670
   BB-guide RNA
   

   Use

   CRISPR

   Tags

   2A-miRFP670 and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh and U6

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-HA-NLGN3 WT

   Plasmid

   #59318

   Purpose

   Expression plasmid of HA-tagged wildtype human Neuroligin 3

   Depositor

   Peter Scheiffele

   Article

   Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

   Insert

   NLGN3 (NLGN3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-HA-rat Nrxn1alpha

   Plasmid

   #58266

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 1alpha

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn1alpha (Nrxn1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   see comments

   Promoter

   CAG

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDMS197

    Plasmid

    #43831

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-con/fon DREADD Gq-mCherry

    Plasmid

    #200661

    Purpose

    Construct for chemogenetically activating and visualizing subpopulations of neurons in the retina

    Depositor

    Samer Hattar

    Insert

    DREADD Gq-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hAdipoq-GFP

    Plasmid

    #176215

    Purpose

    Expresses GFP under the control of distal enhancer and the promoter of human adiponectin gene

    Depositor

    Yu-Hua Tseng

    Article

    Shamsi et al Nat Commun. 2020 Mar 17;11(1):1421. doi:

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCG-TPMV Fd32

    Plasmid

    #163964

    Purpose

    Expression of TPMV F glycoprotein in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

    Insert

    TPMV F glycoprotein
    

    Expression

    Mammalian

    Mutation

    Lacking 32 amino acids at C-terminus

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3-HA PSPC1

    Plasmid

    #101764

    Purpose

    Expresses PSPC1 in mammalian cells

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    paraspeckle component 1 (PSPC1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-DIO_EGFP-GFE3

    Plasmid

    #79871

    Purpose

    For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Has Service

    AAV1

    Insert

    GFP-GFE3
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol2CG2-hsp70I:QFGal4-SV40pA

    Plasmid

    #155120

    Purpose

    Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    hsp70l:QFGal4 (hsp70l Budding Yeast, Neurospora crassa, Zebrafish)
    

    Use

    Zebrafish expression

    Promoter

    hsp70l

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBV65

    Plasmid

    #108363

    Purpose

    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

    Depositor

    Scott Moye-Rowley

    Article

    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

    Insert

    loxP-natMX6-CgMET3prom-cre-loxP
    

    Use

    Gene disruption marker for candida glabrata

    Promoter

    C. glabrata MET3

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Lyn-dInPAkt

    Plasmid

    #181834

    Purpose

    Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dInPAkt
    

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFX-mito-EGFP

    Plasmid

    #159096

    Purpose

    Express EGFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with mitochondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIRCgp41-1

    Plasmid

    #74227

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    gp41-1 C-intein and gp41-1 N-intein

    Expression

    Bacterial

    Promoter

    T7-lac

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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