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We narrowed to 18,759 results for: nls

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Showing: 5161 - 5200 of 18759 results

  7. pYPQ-dSpRY-Act3.0

    Plasmid
    #158416
    Purpose
    CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Publication
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    158416-plasmid-map-sequence-id-337116

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  9. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154787
    Purpose
    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain
    Depositor
    Robert Singer
    Publication
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Synthetic, Fly)
    Use
    Lentiviral
    Tags
    GFP, V5, and stdMCP
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154787-plasmid-map-sequence-id-306329

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  11. pDT305

    Plasmid
    #128801
    Purpose
    Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Depositor
    Bradley Bernstein
    Publication
    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:
    Insert
    dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Use
    CRISPR and Lentiviral
    Promoter
    hUBC promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    128801-plasmid-map-sequence-id-255180

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  12. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid
    #134470
    Purpose
    Human expression vector for SaCas9-HF variant.
    Depositor
    Zongli Zheng
    Publication
    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    Use
    CRISPR
    Tags
    SV40NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    R245A, N413A, N419A, R654A
    Promoter
    CAG
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    134470-plasmid-map-sequence-id-264583

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  18. AAV-KrasG12D/sgKras/Cre

    Plasmid
    #99851
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Publication
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99851-plasmid-map-sequence-id-193760

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  19. dCas9-FOG1[N+C]

    Plasmid
    #100085
    Purpose
    dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FOG1(aa 1-45) (ZFPM1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-FOG1-dCas9-FOG1-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    100085-plasmid-map-sequence-id-197489

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  23. pTetON-DeAct-GS1

    Plasmid
    #89454
    Purpose
    Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells
    Depositor
    Brad Zuchero
    Publication
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DeAct-GS1 (GSN Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Truncation spanning cytoplasmic Gsn amino acids 5…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89454-plasmid-map-sequence-id-176821

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  26. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

    Plasmid
    #84247
    Purpose
    Expresses direct fusion VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-tagBFP-Sp dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), HA tag, VPR, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84247-plasmid-map-sequence-id-160890

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  27. TetO-dCas9-D3A

    Plasmid
    #78254
    Purpose
    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter
    Depositor
    Grant Challen
    Publication
    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A CD aa 598-912 (DNMT3A Human)
    Use
    CRISPR
    Tags
    Flag epitope tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78254-plasmid-map-sequence-id-182128

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  28. pCas9-VRER_2A_GFP

    Plasmid
    #75475
    Purpose
    Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control
    Depositor
    Marc Tessier-Lavigne
    Publication
    Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VRER
    Use
    CRISPR
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, T1337R
    Promoter
    CAG
    Available since
    May 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75475-plasmid-map-sequence-id-142912

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  30. pMCS-rybozyme-IRES-CAS9

    Plasmid
    #64668
    Purpose
    Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.
    Depositor
    Wataru Fujii
    Publication
    Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CAS9
    HDV ribozyme
    Use
    CRISPR
    Tags
    Flag, Internal ribosome entry site, and guide RNA…
    Expression
    Mammalian
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64668-plasmid-map-sequence-id-108759

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  34. pCAG-GBP7-p65AD

    Plasmid
    #49441
    Purpose
    Expression of a GFP-dependent transcription factor component in mammalian cells
    Depositor
    Connie Cepko
    Publication
    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GBP7-p65 transactivation domain fusion protein
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Jan. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49441-plasmid-map-sequence-id-323309

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Showing: 5161 - 5200 of 18759 results