Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 15,323 results for: pET

Clear keywords
Showing: 5161 - 5200 of 15323 results

  4. MZB160

    Plasmid
    #217841
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (L8K+Q71L) for pulldown assays
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    Arf1 (ARF1 Human)
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    L8K, Q71L
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217841-plasmid-map-sequence-id-432998

    Enlarge

  17. MZB224

    Plasmid
    #226998
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    Arf1 (ARF1 Human)
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    T31N
    Promoter
    T7
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    226998-plasmid-map-sequence-id-455977

    Enlarge

  26. pEUK028

    Plasmid
    #180826
    Purpose
    T7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    SwGdmA (SWIT_RS16490 Synthetic, Sphingomonas wittichii RW1)
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180826-plasmid-map-sequence-id-355555

    Enlarge

  27. pEUK037

    Plasmid
    #180829
    Purpose
    T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    CnGdmB (CNE_RS35790 Cupriavidus necator N-1)
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180829-plasmid-map-sequence-id-355562

    Enlarge

  28. RGD-I27-CC

    Plasmid
    #85393
    Purpose
    MTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Tags
    6x His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85393-plasmid-map-sequence-id-172696

    Enlarge

  29. p1G108CFa

    Plasmid
    #85089
    Purpose
    Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The G108C variant of PCNA1 fused to an FAD domain of P450 BM3
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1, A74G/C773S/C810S in FAD domain of…
    Promoter
    T7 promoter
    Available Since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    85089-plasmid-map-sequence-id-164483

    Enlarge

  32. YopH (residues 164-468)

    Plasmid
    #79749
    Purpose
    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    YopH (residues 164-468)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79749-plasmid-map-sequence-id-443904

    Enlarge

  33. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

    Enlarge

  35. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191238-plasmid-map-sequence-id-380469

    Enlarge

Showing: 5161 - 5200 of 15323 results