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164,231 results

Showing: 5241 - 5280 of 164231 results

  1. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available Since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  4. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Article
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-spe…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

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  8. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-487610

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  14. LifeAct-HaloTag

    Plasmid
    #176105
    Purpose
    F-actin marker
    Depositor
    Dorus Gadella
    Insert
    LifeAct
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176105-plasmid-map-sequence-id-346330

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  16. pDestTol2pA2-U6:gRNA

    Plasmid
    #63157
    Purpose
    Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Type
    Empty backbone
    Use
    CRISPR; Tol2 destination vector for gateway
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    63157-plasmid-map-sequence-id-200862

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  19. pcDNA3-TLR7-YFP

    Plasmid
    #13022
    Depositor
    Doug Golenbock
    Insert
    TLR7 (TLR7 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13022-plasmid-map-sequence-id-476228

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  23. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166700-plasmid-map-sequence-id-329224

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  26. WT-MEK1-V5

    Plasmid
    #217297
    Purpose
    Lentivirus vector for expression of MEK1
    Depositor
    Channing Der
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    Promoter
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217297-plasmid-map-sequence-id-431009

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  31. pLJM1-FIRE-pHLy

    Plasmid
    #170775
    Purpose
    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    CMV
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170775-plasmid-map-sequence-id-339023

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  32. pCMV-Luc

    Plasmid
    #45968
    Depositor
    Ralf Kuehn
    Insert
    Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    45968-plasmid-map-sequence-id-64565

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  35. pCDH-EF1-rtTA-PGK-BSD

    Plasmid
    #192885
    Purpose
    expression of rtTA
    Depositor
    Kazuhiro Oka
    Insert
    rtTA
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    192885-plasmid-map-sequence-id-378874

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Showing: 5241 - 5280 of 164231 results