159,258 results

1. pMXs-3XFLAG-EGFP-OMP25

   Plasmid

   #83354

   Purpose

   For tagging mitochondria with Sigma FLAG epitopes

   Depositor

   David Sabatini

   Article

   Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

   Insert

   3XFLAG-EGFP-OMP25
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+8NmCherry-ABCB1a

   Plasmid

   #34939

   Depositor

   Amro Hamdoun

   Article

   Gokirmak et al J Biol Chem. 2012 Nov 2.

   Insert

   ABCB1a
   

   Use

   Sea urchin, zebrafish, xenopus

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   Jan. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

3. FnR9-10

   Plasmid

   #182567

   Purpose

   Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 9-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PLlacO-1-CImf

   Plasmid

   #40126

   Depositor

   Michel Maharbiz

   Article

   Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

   Insert

   Lac inducible lambda repressor
   

   Use

   Synthetic Biology

   Tags

   mf-lon ssrA tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG424-10X-tRNA

   Plasmid

   #70125

   Purpose

   Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

   Depositor

   Jay Hesselberth

   Article

   Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

   Insert

   10X-tRNA block
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   None – SUP4 pol III promoter provided by insert

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pTT3-SP-6xHis-KIR2DL5(FL)-FLAG

   Plasmid

   #157623

   Purpose

   Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DL5 (KIR2DL5A Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. eGFP-luciferase-C1

   Plasmid

   #163523

   Purpose

   Expresses both eGFP and firefly luciferase in mammalian cells

   Depositor

   Thomas Weber

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

   Plasmid

   #83919

   Purpose

   Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   DsRed-Express2
   Bsr
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A

   Expression

   Mutation

   Promoter

   hUbC

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-neo:alpha4-mcherry

   Plasmid

   #45073

   Depositor

   Henry Lester

   Article

   Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

   Insert

   alpha4-mcherry (Chrna4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLNHA-C1-HsPatL1_I

    Plasmid

    #146544

    Purpose

    Mammalian Expression of HsPatL1

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsPatL1 (PATL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. RNASEL gRNA (BRDN0001149358)

    Plasmid

    #77052

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTH1-SaSrtA

    Plasmid

    #64973

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    T7 promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABE9

    Plasmid

    #194208

    Purpose

    Mammalian expression of ABE9

    Depositor

    Dali Li

    Article

    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

    Insert

    TadA-8e (N108Q/L145T)-nSpCas9
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EFIa-iTGB1

    Plasmid

    #115799

    Purpose

    Integrin B1 overexpression

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    iTGB1 (ITGB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Thy1.1-G5

    Plasmid

    #234839

    Purpose

    pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

    Depositor

    Gabriel Victora

    Article

    Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

    Insert

    Thy1.1 (Thy1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-P2A-5xGly

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHK316

    Plasmid

    #235474

    Purpose

    Inducible gene VIII by Ptet promoter

    Depositor

    Yolanda Schaerli

    Article

    et al Nat Commun 16, 3569 (2025)

    Insert

    phage M13 geneVIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHK352.3

    Plasmid

    #235473

    Purpose

    Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)

    Depositor

    Yolanda Schaerli

    Article

    et al Nat Commun 16, 3569 (2025)

    Insert

    sgRNA3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. HP17deltaP8

    Plasmid

    #235447

    Purpose

    Helper plasmid for regulated phage transfer

    Depositor

    Yolanda Schaerli

    Article

    et al Nat Commun 16, 3569 (2025)

    Insert

    Coding part of the M13KO7 helper phage genome, except gene VIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRX-IP-EGFP-PA-BAX(mito)

    Plasmid

    #228942

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-mTSP33

    Plasmid

    #236541

    Purpose

    For expression of Tspan33 in mammalian cells

    Depositor

    Sandra Citi

    Article

    Shah et al Cell Rep. 2018 Nov 20;25(8):2132-2147.e7

    Insert

    TSPAN33 (Tspan33 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJPC210

    Plasmid

    #234532

    Purpose

    Int13 Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJPC063

    Plasmid

    #234533

    Purpose

    TRV2 expressing Int13

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    Integrase 13 recombinase
    

    Use

    Recombinase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJPC062

    Plasmid

    #234534

    Purpose

    TRV2 expressing CinH

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    CinH Recombinase
    

    Use

    Recombinase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJPC430

    Plasmid

    #234529

    Purpose

    CinH Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pdCRISPomyces-2BD

    Plasmid

    #234429

    Purpose

    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

    Depositor

    Min-Kyu Oh

    Article

    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

    Insert

    Modified dCas9(D10A, H840A) with polyaspartate linking
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Linked polyaspartate using glycine-serine linker

    Promoter

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 78H05-FRT100-STOP-FRT100-p65.AD

    Plasmid

    #232828

    Purpose

    Enhancer 78H05 driven p65.AD, gated by Sparse.FRT100

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT100-STOP-FRT100
    p65.AD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBP-Sparse.FRT10-p65.AD

    Plasmid

    #232830

    Purpose

    Enhancer-free Sparse.FRT10 p65.AD with ccdB for Gateway

    Depositor

    Liqun Luo

    Article

    Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

    Inserts

    FRT10-STOP-FRT10
    p65.AD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAROTENE5X

    Plasmid

    #208816

    Purpose

    Encodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell

    Depositor

    Marc Erhardt

    Article

    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

    Insert

    Five copies of JUB1 binding site within the synthetic promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    JUB1 TF-responsive promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pETM6-T7-VHH72

    Plasmid

    #202487

    Purpose

    Expresses the nanobody VHH72 with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VHH72
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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