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pCMV6-AN-DDK_S520A POLI

Plasmid

#131237

Purpose

Mammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a point mutation converting Pol iota ser…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_S580A POLI

Plasmid

#131238

Purpose

Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a point mutation converting Pol iota ser…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_3KA POLI

Plasmid

#131239

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert lysine resi…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCri-9a

Plasmid

#61318

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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tdTomato-N1

Plasmid

#54642

Purpose

Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-STING_gRNA3

Plasmid

#127640

Purpose

Knock-out of human STING

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.pU1a-SpCas9

Plasmid

#121507

Purpose

Expresses codon-optimized SpCas9 in mammalian cells.

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

SpCas9

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

U1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL hygro H-Ras V12

Plasmid

#18749

Depositor

Scott Lowe

Article

Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Insert

H-Ras V12 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

V12

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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qMaLioffG

Plasmid

#212825

Purpose

green fluorescent FLIM indicator for endogenous ATP imaging

Depositor

Tetsuya Kitaguchi

Article

Arai et al Nat Commun. 2025 Nov 13;16(1):9972. doi:

Insert

qMaLioffG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

Plasmid

#204189

Purpose

Doxycycline inducible lentiviral vector for human GATA6 overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Hygro DEST (w530-1)

Plasmid

#19066

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pmiRFP670-N1

Plasmid

#79987

Purpose

Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Has Service

Cloning Grade DNA

Insert

miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-STING-sh5

Plasmid

#127647

Purpose

Knock-down of human STING

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

STING shRNA (STING1 Human)

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDY1440 STITCHR GFP NOLC1 payload

Plasmid

#234829

Purpose

GFP cargo with 50bp NOLC homology arms

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

copGFP

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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OPRM1-Tango

Plasmid

#66464

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

OPRM1 (OPRM1 Human)

Tags

FLAG and tTA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-Sec61b WPRE

Plasmid

#236228

Purpose

AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-Sec61b (SEC61B Synthetic, Human)

Use

AAV

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

Plasmid

#40640

Depositor

Brenda Lilly

Article

Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

Insert

NICD3 (NOTCH3 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4A

Plasmid

#224448

Purpose

Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYNSL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNICE-NL2A

Plasmid

#15259

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 2 with insert A (Nlgn2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pEGIP

Plasmid

#26777

Purpose

Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

EF1a-GFP-IRES-Puro (gfp )

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP

Plasmid

#62231

Purpose

Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Tags

mGFP

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Article

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

p65 (RELA Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-delta30-TFEB

Plasmid

#44445

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 1-30; H32D

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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DD-DamV133A-IRES2-mCherry

Plasmid

#159600

Purpose

Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the V133A mutant of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamV133A-IRES2-mCherry

Expression

Mammalian

Mutation

Dam V133A mutation

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-V106W

Plasmid

#215825

Purpose

Express CBE6b V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-80L-V4-ELP-EEP13

Plasmid

#237223

Purpose

Expresses micelle-forming elastin-like polypeptide (ELP)-endosomal escape peptide (EEP) fusion construct in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

V4-ELP-EEP13

Tags

6xHis

Expression

Bacterial

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-MFSD8(L9)-Zeo

Plasmid

#239947

Purpose

Expression of MFSD8 loop 9 tagged with rabbit IgG Fc2

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

Fc2-MFSD8(L9)

Expression

Bacterial and Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pICH47742::p35S-ePEmax3::tEURb7

Plasmid

#227692

Purpose

Golden Gate level 1, position 2, ePEmax3 expression cassette.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).

Use

CRISPR

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mTFP1-N1

Plasmid

#54521

Purpose

Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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