We narrowed to 8,907 results for: Ott

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EphA2-YFP

Plasmid

#108852

Purpose

Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

Depositor

Kalina Hristova

Article

Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

Insert

EPHA2 (EPHA2 Human)

Tags

Labeled with eYFP on the C-Terminus via a 15 amin…

Expression

Mammalian

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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EFIa-iTGB1

Plasmid

#115799

Purpose

Integrin B1 overexpression

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

iTGB1 (ITGB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8s

Plasmid

#169247

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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TetON-shYAP

Plasmid

#115667

Purpose

Inducible YAP knockdown

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP shRNA (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST pcDNA5-FRT/TO-eGFP-Rif1

Plasmid

#52506

Purpose

mammalian expression vector of Rif1 siRNA resistant

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

RIF1 (RIF1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

siRNA resistant sequence: ATAGTAC

Available Since

June 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Flag Leucyl tRNA synthetase pRK5

Plasmid

#46341

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

leucyl tRNA synthetase (LARS1 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

Plasmid

#61593

Purpose

A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Inserts

hSaCas9
Chimeric guide for SaCas9

Use

AAV and CRISPR

Tags

HA, NLS, and OLLAS tag

Expression

Mammalian

Mutation

K175R and K736R (deUb mutations)

Available Since

Feb. 16, 2015

Availability

Academic Institutions and Nonprofits only

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HA Depdc5 pRK5

Plasmid

#46327

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

HA

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Flag Depdc5 pRK5

Plasmid

#46340

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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pSS02:cyt-Peredox-mCherry

Plasmid

#161743

Purpose

cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

Depositor

Markus Schwarzländer

Article

Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

Insert

cyt-Peredox-mCherry

Expression

Plant

Promoter

Ubiquitin 10

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTTCC

Plasmid

#164264

Purpose

Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

Depositor

Gudmundur Hreggvidsson

Article

Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

Inserts

Cas9
gRNA scaffold

Expression

Bacterial

Promoter

Pcel and trc

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCold-I-Dsup

Plasmid

#90021

Purpose

To express His-tagged Dsup in bacteria

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Tags

His

Expression

Bacterial

Promoter

cspA

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET-21a_3xNLS_SpCas9_protein_expression

Plasmid

#114365

Purpose

Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

Depositor

Scot Wolfe

Article

Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

Insert

3xNLS_SpCas9

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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Ef1a-DIO-BiPOLES-mCerulean

Plasmid

#154949

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLv-HSA-uDys/eGFP

Plasmid

#26810

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

dystrophin (DMD Human)

Use

Lentiviral; Lentiviral plasmid backbone from l. n…

Tags

eGFP

Mutation

deleted R4-R23 deleted exons 71-78

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1

Plasmid

#218796

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1

Expression

Mammalian

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro XLone-CCND2

Plasmid

#179845

Purpose

donor plasmid for inducible expression of CCND2 in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Expression

Mammalian

Promoter

TRE3GS

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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H11-mScarletALG8

Plasmid

#172440

Purpose

Hipp11 targeting vector, expresses mScarlet-mALG8

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-mALG8

Use

Mouse Targeting

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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hCathepsin B

Plasmid

#11249

Purpose

Mammalian expression of human cathepsin B

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

Insert

cathepsin B (CTSB Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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LV-PGK-Cre

Plasmid

#172433

Purpose

Lentivirus, expresses Cre recombinase

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

Cre

Use

Lentiviral

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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EphA2-MT

Plasmid

#108851

Purpose

Encodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

Depositor

Kalina Hristova

Article

Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

Insert

EPHA2 (EPHA2 Human)

Tags

Labeled with mTurquoise on the C-Terminus via a 1…

Expression

Mammalian

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pF4Ag NanoLuc (ATG-42)

Plasmid

#137777

Purpose

Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

Depositor

Lance Encell

Article

Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

Insert

NanoLuc (Nluc)

Expression

Bacterial and Mammalian

Mutation

NanoLuc contains 16 amino acid changes compared t…

Promoter

T7 and CMV

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pB-TopFlash-NanoLucP

Plasmid

#204347

Purpose

TopFlash reporter

Depositor

Li-Fang Chu

Article

Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

Insert

TopFlash-NanoLucP

Use

Luciferase

Tags

NanoLucP

Promoter

miniP

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hCathepsin L

Plasmid

#11250

Purpose

Mammalian expression of human cathepsin L

Depositor

Hyeryun Choe

Article

Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

Has Service

Cloning Grade DNA

Insert

cathepsin L (CTSL Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-All-in-one-hCascade

Plasmid

#134919

Purpose

Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMTS_mScarlet-i_N1

Plasmid

#85059

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

MTS (COX8A Human)

Tags

mScarlet-i

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 FLAG FBXW7alpha

Plasmid

#236470

Purpose

transient overexpression of FBXW7alpha in mammalian cells

Depositor

Michele Pagano

Article

Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

Insert

FBXW7alpha (FBXW7 Human)

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN4-CMV-K2M

Plasmid

#21164

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Klf4, cMyc (MYC, KLF4 Human)

Use

Lentiviral; Reprogramming

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-Neo_CAG-Cas9

Plasmid

#86698

Purpose

Cas9 expression vector for targeting to the AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

pCag

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6.2EmGFP-DAXX

Plasmid

#169726

Purpose

Mammalian expression construct of GFP tagged full length DAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX (DAXX Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
RpH-ILV-3xALFA

Plasmid

#225135

Purpose

RpH-ILV probe strongly colocalises with lysosomal markers.

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

TMEM192 (TMEM192 Human)

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-N-HA-NEK7

Plasmid

#75142

Purpose

Expresses N-terminal HA-tagged NEK7 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-FDIO-ArchT-GFP

Plasmid

#124640

Purpose

AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

ArchT

Use

AAV; Flp/frt

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-TRSter

Plasmid

#78865

Purpose

Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

Depositor

Elias Arnér

Article

Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

Insert

Thioredoxin Reductase (Txnrd1 Rat)

Tags

Mutated E. coli-type SECIS element (3’ end of ins…

Expression

Bacterial

Promoter

T7lac

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pATZ-BFP-HA-GFP11

Plasmid

#177721

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

GFP11, HA, and TagBFP

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFLAG-VP64-SadCas9-NLS-VP64

Plasmid

#135338

Purpose

3xFLAG 2xVP64 SadCas9 for single guide cloning

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

SadCas9

Tags

3xFLAG-VP64-SV40 NLS and NLS-VP64

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

Plasmid

#174515

Purpose

Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

Mm PylS
MmtRNA-Pyl-opt(UGA)

Expression

Bacterial

Mutation

ASL from the serT gene (UGA anticodon) and recoded

Promoter

GlnS and lpp

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTS_mScarlet_N1

Plasmid

#85057

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

MTS (COX8A Human)

Tags

mScarlet

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ataxin-3

Plasmid

#89975

Purpose

Expresses GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 8,907 results for: Ott