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Showing: 6651 - 6700 of 60900 results
  1. C-BLa 2.0

    Plasmid
    #92060
    Purpose
    The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.
    Depositor
    Insert
    C-BLa 2.0 fusion protein
    Tags
    Flag (DYKDHDG)
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pMTP130 (pTA106_TnsABC(Tn6900))

    Plasmid
    #164261
    Purpose
    Vector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.
    Depositor
    Insert
    tnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
    Expression
    Bacterial
    Mutation
    (tnsA) Changed Alanine 125 to Aspartic acid
    Promoter
    Lactose
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pJK503

    Plasmid
    #155380
    Purpose
    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available Since
    May 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47450
    Purpose
    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Insert
    TALE BB(N136_0.5HD_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
  5. IpO

    Plasmid
    #48233
    Purpose
    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.
    Depositor
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    Use
    Routine cloning vector
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    URA3 promoter and none
    Available Since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid
    #107421
    Purpose
    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Insert
    SpyCatcher
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Mutation
    mutation of glutamate 77 to glutamine
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pIBA2-SpyTag-MBP

    Plasmid
    #107422
    Purpose
    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.
    Depositor
    Insert
    Maltose-binding protein (MalE)
    Tags
    OmpA signal peptide, SpyTag, and StrepII
    Expression
    Bacterial
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pEPQD1CB0121

    Plasmid
    #177053
    Purpose
    MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter
    Depositor
    Insert
    iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pEPQD1CB0117

    Plasmid
    #177049
    Purpose
    MoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoter
    Depositor
    Insert
    iridoid synthase (CrISY) from Catharanthus roseus
    Use
    Synthetic Biology
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pOPO636

    Plasmid
    #195299
    Purpose
    pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).
    Depositor
    Insert
    TnsABCQ operon of McCAST (Tn7575)
    Expression
    Bacterial
    Promoter
    IPTG inducible lac promoter
    Available Since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 6651 - 6700 of 60900 results