We narrowed to 9,766 results for: 158

1. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

   Plasmid

   #107581

   Purpose

   B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-BLM DM

   Plasmid

   #80071

   Purpose

   Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

   Depositor

   Nathan Ellis

   Article

   Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

   Insert

   BLM (BLM Human)
   

   Use

   Tags

   GFP-BLM

   Expression

   Mammalian

   Mutation

   K317R (AAA>AGA) and K331R (AAA>AGA)

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOGS539_GLuc-ns

   Plasmid

   #226719

   Purpose

   Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Gaussia Luciferase
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pN094

   Plasmid

   #167155

   Purpose

   MoClo-compatible Level1 (position 6) vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of 0.4 kb 35S promoter, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   p35s-npgA-ocsT
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   p35s

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SECURE miniABEmax-V82G (pJUL1828)

   Plasmid

   #131313

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-Lepr_Short-IRES-eGFP

   Plasmid

   #196935

   Purpose

   Doxycycline inducible expression of short Lepr (no signaling) with eGFP marker

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   Lepr (Lepr Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   short isoform, lack of signaling casacade

   Promoter

   TRE

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpG-PPE

   Plasmid

   #170130

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

   Insert

   nCas9-SpG(H840A)-M-MLV
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…

   Promoter

   maize Ubiquitin-1

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p21-WT-MEFV

   Plasmid

   #134702

   Purpose

   Lentiviral expression of human MEFV after doxycycline administration

   Depositor

   Thomas Henry

   Article

   Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

   Insert

   MEFV (MEFV Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mutation

   WT

   Promoter

   TRE2 (Dox-inducible)

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mARGAna (cassette 1, transient)

   Plasmid

   #197588

   Purpose

   Second-generation mammalian acoustic reporter gene derived from Anabaena

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   mARGAna (cassette 1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

    Plasmid

    #132686

    Purpose

    AAV plasmid encoding mouse ACY1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Acylase I (Acy1, Acy1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

    Plasmid

    #133044

    Purpose

    Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-ETWV
    

    Use

    Tags

    NLS, TEV protease + TEV cleavage site, and miRFP6…

    Expression

    Mammalian

    Mutation

    S63K

    Promoter

    CAG

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. ASC-CASP1 Octamer

    Plasmid

    #164032

    Purpose

    Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CASP1, ASC (CASP1, PYCARD Human)
    

    Use

    Tags

    His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    W169G (ASC), G20K (CASP1)

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

    Plasmid

    #163572

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MRP1-CD4d3+4-bio

    Plasmid

    #73120

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding MRP1 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    MRP1 (ABCC1 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAPM-miR30-IFIH1-ts1

    Plasmid

    #174259

    Purpose

    IFIH1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    IFIH1 shRNA (IFIH1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG

    Plasmid

    #81022

    Purpose

    Cancer-associated ASXL1 truncation mutant in retroviral vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.Y591X truncation

    Promoter

    5’LTR

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid

    #111573

    Purpose

    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Mutation

    lysine to glutamine mutation at the following ami…

    Promoter

    polyhedrin promoter

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJT302 Ec03 Lenti pEF-attB LSR GFP

    Plasmid

    #184945

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Patrick Hsu, Ami Bhatt, Lacra Bintu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec03 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT303 Ec04 Lenti pEF-attB LSR GFP

    Plasmid

    #184946

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Patrick Hsu, Ami Bhatt, Lacra Bintu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec04 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABE-puro-Halo-hMRE11(wt)

    Plasmid

    #208393

    Purpose

    To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H129N

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBABE-puro-Halo-hMRE11(H129N)

    Plasmid

    #208394

    Purpose

    To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H129N

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. plenti-px330-CRBN-T2-pGK-Pur

    Plasmid

    #107383

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pJM186A

    Plasmid

    #218127

    Purpose

    Hybrid circuit displaying hsCRBN bait and driving expression of gIII

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    M13 pIII
    

    Use

    Tags

    434cI_RR69

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET28-HADH2

    Plasmid

    #67863

    Purpose

    bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    HADH2 (HSD17B10 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCDNA3.1 hSCN5A-L325R

    Plasmid

    #145373

    Purpose

    expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

    Depositor

    Hugues Abriel

    Article

    Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 325 to Arginine

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

    Plasmid

    #74261

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.Y591X truncation

    Promoter

    CMV

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

28. TGMP.shKras.1422

    Plasmid

    #59913

    Purpose

    Tet on shKras for conditional Kras knockdown

    Depositor

    Tyler Jacks

    Article

    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

    Insert

    Kras (Kras Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET30a-His6-ABP-LOXCATmut

    Plasmid

    #139972

    Purpose

    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

    Plasmid

    #163545

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge