164,243 results

1. pRK5F-TACE

   Plasmid

   #31713

   Purpose

   Mammalian expression of human TACE (ADAM17)

   Depositor

   Rik Derynck

   Article

   Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

   Insert

   TACE (ADAM17 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCDNA3.1_3XFlag

   Plasmid

   #182494

   Purpose

   Empty plasmid containing Flag tag for C-terminal tagging

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

   Insert

   3xFlag with 5 Glycolic spacer
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

   Plasmid

   #208377

   Purpose

   Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

   Depositor

   Daniel Hawiger

   Article

   Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human CMV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCKO2

   Plasmid

   #125518

   Purpose

   Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

   Depositor

   Jason Moffat

   Article

   Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138151

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry Paxillin

   Plasmid

   #50526

   Depositor

   Kenneth Yamada

   Insert

   PXN paxillin (PXN Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRA110

   Plasmid

   #209343

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mStayGold fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe puro IRES-EGFP

   Plasmid

   #14430

   Depositor

   L. Miguel Martins

   Has Service

   Cloning Grade DNA

   Insert

   IRES-EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDK12 gRNA (BRDN0001147156)

   Plasmid

   #75915

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK12

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK12 (CDK12 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-KIT-V5/HIS

    Plasmid

    #201986

    Purpose

    expression of human KIT receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    KIT receptor tyrosine kinase (KIT Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

    Plasmid

    #204579

    Purpose

    lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBluescriptKS(-)-FRB

    Plasmid

    #104475

    Purpose

    Plasmid carrying FRB with C-terminal HA tag.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    FRB (MTOR Human)
    

    Use

    Phagemid

    Tags

    HA tag

    Expression

    Mutation

    includes only the FRB domain

    Promoter

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CDK12 gRNA (BRDN0001147294)

    Plasmid

    #75916

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK12

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK12 (CDK12 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCold I-Hero45

    Plasmid

    #187931

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero45

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero45 (SERBP1 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

    Plasmid

    #188778

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-lyso-pHluorin

    Plasmid

    #70113

    Purpose

    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHluorin (Cd63 Aequorea victoria, Mouse)
    

    Use

    Tags

    HA-Tag and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti puro HA-Ubiquitin

    Plasmid

    #74218

    Purpose

    Lentiviral expression of HA tagged Ubiquitin

    Depositor

    Melina Fan

    Insert

    ubiquitin (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Single ubiquitin coding sequence (full length UBB…

    Promoter

    CMV

    Available Since

    March 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. PHR-GFP-FUSN-VP16

    Plasmid

    #183933

    Purpose

    Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-FUSN-VP16 (CRY2, UL48 Mustard Weed, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGWB452

    Plasmid

    #74834

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC-LYCipi

    Plasmid

    #53279

    Purpose

    Contains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-S5E2-GFP-fGFP

    Plasmid

    #135631

    Purpose

    AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, and AAV9

    Insert

    GFP-fGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2 Notch1 Full Length-6MT

    Plasmid

    #41728

    Depositor

    Raphael Kopan

    Article

    Schroeter et al Nature. 1998 May 28;393(6683):382-6.

    Insert

    Notch-1 Full Length (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    L1997V; Contains murine Notch-1 protein up to ami…

    Promoter

    Available Since

    Dec. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EGFP-FKBP(M)x4

    Plasmid

    #85003

    Purpose

    Expresses EGFP-FKBP(M)x4 in yeast

    Depositor

    Benjamin Glick

    Article

    Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

    Insert

    EGFP-FKBP(M)x4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-CAG-UnaG-P2A-FusionRed

    Plasmid

    #191109

    Purpose

    Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    UnaG
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWR76

    Plasmid

    #202776

    Purpose

    episomal fluorescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCherry
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBlu-CGT-1

    Plasmid

    #170158

    Purpose

    Expresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coli

    Depositor

    Sarah MacDonald

    Insert

    Cyclomaltodextrin Glucanotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TFORF3536

    Plasmid

    #145012

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXD4 (HOXD4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMBP-HRV3C-AcrIF9

    Plasmid

    #141442

    Purpose

    Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

    Depositor

    Blake Wiedenheft

    Article

    Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

    Insert

    AcrIF9
    

    Use

    Tags

    HRV3C cleavage site and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BB2_AB

    Plasmid

    #98534

    Purpose

    empty BB2 for transcription unit cloning

    Depositor

    Brigitte Gasser, Michael Sauer, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223_EGFR_p.G719D

    Plasmid

    #81708

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    G719D

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGfaABC1D-nLac

    Plasmid

    #53131

    Purpose

    expression of lacZ driven by human GfaABC1D promoter

    Depositor

    Michael Brenner

    Article

    Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains ABC1D regions of Gfa2 promoter, -1757 to…

    Promoter

    human Gfa2

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-ExRai-AktAR2

    Plasmid

    #184047

    Purpose

    Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    ExRai-AktAR2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMVβ-p300-myc

    Plasmid

    #30489

    Purpose

    Mammalian expression of human p300 with myc tag

    Depositor

    Tso-Pang Yao

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTNS1

    Plasmid

    #64967

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 23, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pFC333

    Plasmid

    #87844

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSL1425 (pSPIN, pCDF backbone)

    Plasmid

    #160735

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

    Plasmid

    #34877

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Peft-3 GFP H2B tbb-2 UTR
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    S65C

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Feb. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-PE6g

    Plasmid

    #207857

    Purpose

    Mammalian expression of PE6g prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6g
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

    Plasmid

    #83841

    Purpose

    Fluorescent probe for G1/S transition and M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pScaf

    Plasmid

    #111401

    Purpose

    Phagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge