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We narrowed to 9,346 results for: UTY

Showing: 7051 - 7100 of 9346 results

  2. pZH520

    Plasmid
    #102673
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102673-plasmid-map-sequence-id-199588

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  5. pZH521

    Plasmid
    #102674
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102674-plasmid-map-sequence-id-199585

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  6. pHR_PGK_SNIPR_synRobo

    Plasmid
    #188340
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Robo1-ECD, a Robo1-TMD, a Robo1-JMD, and a Gal4VP64 transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_Robo1-ECD_Robo1-TMD_Robo1-JMD_Gal4VP64
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188340-plasmid-map-sequence-id-370786

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  9. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid
    #200408
    Purpose
    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    iRFP720 (frameshift mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200408-plasmid-map-sequence-id-396809

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  11. nGR2175-gs10-NLS-VPR

    Plasmid
    #188264
    Purpose
    Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    nGR2175
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188264-plasmid-map-sequence-id-374534

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  13. pDOC-K-glmS-mPlum

    Plasmid
    #158062
    Purpose
    Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Insert
    monomeric plum fluoresecent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    acpP
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158062-plasmid-map-sequence-id-309442

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  15. pZH522

    Plasmid
    #102675
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102675-plasmid-map-sequence-id-199391

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  20. pZA-GFP-R5

    Plasmid
    #106256
    Purpose
    Constitutively expresses GFP fused with silaffin-R5 silica affinity peptide
    Depositor
    Urartu Seker
    Article
    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi
    Insert
    Green fluorescent protein
    Use
    Tags
    Added silaffin R5 peptide to C terminus of GFP af…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    106256-plasmid-map-sequence-id-232729

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  21. HA-hDAGLb-V5

    Plasmid
    #87675
    Purpose
    Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
    Depositor
    Pat Doherty
    Insert
    Human Diacylglycerol Lipase Beta (DAGLB Human)
    Use
    Tags
    HA and V5 tag
    Expression
    Mammalian
    Mutation
    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…
    Promoter
    T7
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87675-plasmid-map-sequence-id-173605

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  23. pMOD8A-RGR-r10

    Plasmid
    #74370
    Purpose
    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    RGR-r10
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74370-plasmid-map-sequence-id-141329

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  33. IBMc233

    Plasmid
    #161939
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PPhlF
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161939-plasmid-map-sequence-id-327549

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  34. pMOD8A-iRGR-r11

    Plasmid
    #74369
    Purpose
    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    RGR-r11
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    AHD1
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74369-plasmid-map-sequence-id-141324

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  37. pJUMP27ts[mCherry]-1A(lacZ)

    Plasmid
    #126980
    Purpose
    Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.
    Depositor
    Chris French
    Article
    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    RepA A56V thermosensitive mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126980-plasmid-map-sequence-id-248074

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  41. pSB1A3-AD010

    Plasmid
    #116851
    Purpose
    Expresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010
    Depositor
    Friedrich Simmel
    Article
    Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415
    Inserts
    AraC
    alpha-HL
    mScarletI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    116851-plasmid-map-sequence-id-226481

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  44. LUC56 (Luc/UGA/SP)

    Plasmid
    #112147
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258mutated to selenocysteine (U)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112147-plasmid-map-sequence-id-214894

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  45. IRE-nonOpt-TREAT

    Plasmid
    #196922
    Purpose
    The codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.
    Depositor
    Jeffrey Chao
    Article
    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.
    Insert
    non-optimal Renilla luciferase
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196922-plasmid-map-sequence-id-387473

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  50. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid
    #73220
    Purpose
    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Use
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PpsbA2
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73220-plasmid-map-sequence-id-134711

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Showing: 7051 - 7100 of 9346 results