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We narrowed to 9,586 results for: Coli

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Showing: 7251 - 7300 of 9586 results

  4. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

    Plasmid
    #127369
    Purpose
    Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Plant
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Arabidopsis UBQ10 promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127369-plasmid-map-sequence-id-246339

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  5. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid
    #127353
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and mVenus
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127353-plasmid-map-sequence-id-246311

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  6. pEND-321_Cacnes_ori

    Plasmid
    #225603
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225603-plasmid-map-sequence-id-451386

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  8. pENTR_L1-YFP-miniTurbo-NES-L2

    Plasmid
    #127351
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127351-plasmid-map-sequence-id-246289

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  9. p3R112C/T180CBM3

    Plasmid
    #85090
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to P450 BM3
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3
    Promoter
    T7 promoter
    Available Since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85090-plasmid-map-sequence-id-164484

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  19. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid
    #127355
    Purpose
    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and mVenus
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127355-plasmid-map-sequence-id-246258

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  20. hCD12

    Plasmid
    #225541
    Purpose
    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi
    Insert
    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    225541-plasmid-map-sequence-id-446604

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  24. pPEPZ-sgRNAclone

    Plasmid
    #141090
    Purpose
    This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107
    Insert
    mCherry
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    p3
    Available Since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    141090-plasmid-map-sequence-id-330475

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  36. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid
    #218778
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(3)
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218778-plasmid-map-sequence-id-444099

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  37. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available Since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  47. pLyGo-Ec-7

    Plasmid
    #163135
    Purpose
    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Nanobody, C-IgAP autotransporter
    Expression
    Bacterial
    Promoter
    P(rhaB)
    Available Since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163135-plasmid-map-sequence-id-332144

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  48. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

    Plasmid
    #127359
    Purpose
    gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127359-plasmid-map-sequence-id-246306

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Showing: 7251 - 7300 of 9586 results