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We narrowed to 8,062 results for: gnal

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pLL3.7m-psMEK2

Plasmid

#89363

Purpose

A lenti-viral vector expressing photoswitchable MEK2 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK2 (MAP2K2 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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ELK3_pet28a

Plasmid

#131651

Purpose

Bacterial expression of ETS gene ELK3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK3 (ELK3 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Plasmid

#173871

Purpose

Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 hFZD4

Plasmid

#115908

Purpose

Expresses human FZD4 in mammalian cells.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

FZD4 (FZD4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-Bos-FLAG MDA5 MI

Plasmid

#52875

Purpose

Expresses MDA5 with a mutation to motif I in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

IFIH1 (IFIH1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K335A, E658K

Promoter

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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myc-Atg14

Plasmid

#86747

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Atg14 (ATG14 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Rab8WT(Flag) in pcDNA3.1neo

Plasmid

#46783

Purpose

expression of WT Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A (RAB8A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-FLCN

Plasmid

#72290

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-HLA-Flag-natT1R2

Plasmid

#113947

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

residues 22-839

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF10_HA_BASU

Plasmid

#153980

Purpose

Express Orf10 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf10 (ORF10 Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLPCX-Cx43-IRES-GFP

Plasmid

#65433

Purpose

Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a-QL

Plasmid

#86076

Purpose

A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed glutamine 67 to leucine

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-T308A/S473A

Plasmid

#49191

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Expression

Mutation

T308A; S473A

Promoter

T7

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCEP4-HLA-cmyc-optiT1R3 a21-852

Plasmid

#113948

Purpose

mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Use

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 21-852

Promoter

CMV

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FNIP2

Plasmid

#72294

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
EGFP-Rab8a-TN

Plasmid

#86077

Purpose

A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed threonine 22 to aspargine

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHR Grb2-TagBFP

Plasmid

#188631

Purpose

Human Grb2 C-terminally labeled with TagBFP

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

GRB2 (GRB2 Synthetic, Human)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Flag WDR24 pRK5

Plasmid

#46334

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR24 (WDR24 Human)

Use

Tags

FLag

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pH1R-P2A-mCherry-N1

Plasmid

#84330

Purpose

Co-expresses (untagged) Histamine-1 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H1R-P2A-mCherry (HRH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PIF3-mEGFP

Plasmid

#100283

Purpose

Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome interacting factor 3 (PIF3 Mustard Weed)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

N-terminus (1-100 aa) of PIF3 is used

Promoter

CAG promoter

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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JpExpress404 PTEN-LONG

Plasmid

#49417

Purpose

Bacterial Expression Vector

Depositor

Ramon Parsons

Article

Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

Insert

PTEN-Long (PTEN )

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pNS20-SpCas9-SNAP

Plasmid

#113717

Purpose

Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag Grb10 wt long

Plasmid

#37481

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 (GRB10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

isoform a, 594aa

Promoter

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-FGL

Plasmid

#190234

Purpose

AAV vector, high-level expression of myrGFP with a somatodendritic-targeting signal (FGL) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

LDLR C-terminal sequence and myristoylation signa…

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2V678F-VSV

Plasmid

#139349

Purpose

Expression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)

Depositor

Sandra Pellegrini

Article

Gakovic et al J Biol Chem. 2008 Jul 4;283(27):18522-9.

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

V362F-V678F

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Actin

Plasmid

#181966

Purpose

Human beta actin tagged with GFP11.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-Actin (ACTB Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-neo beta catenin delta45

Plasmid

#16520

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

beta catenin (CTNNB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Delta 45 (HCT116 mutation)

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pBSFI-myr-akt-T308D/S473D

Plasmid

#49192

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Expression

Mutation

T308D; S473D

Promoter

T7

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO/GFP-ZNF598

Plasmid

#141191

Purpose

Expresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

ZNF598 (ZNF598 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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POLD1_pLX307

Plasmid

#98358

Purpose

Lentiviral expression of POLD1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

POLD1 (POLD1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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