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We narrowed to 16,886 results for: Por

Showing: 7301 - 7350 of 16886 results

  8. pET24a-LaM4-2xTS

    Plasmid
    #162779
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162779-plasmid-map-sequence-id-321884

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  18. PB-TAC-OKMS-KRAB-dCas9

    Plasmid
    #120356
    Purpose
    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OKMS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [10-483]
    Promoter
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120356-plasmid-map-sequence-id-236039

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  19. PB-TAC-OK+9MS-KRAB-dCas9

    Plasmid
    #120357
    Purpose
    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OK+9MS cassette
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Klf4 [1-483]
    Promoter
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120357-plasmid-map-sequence-id-236025

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  20. THI ONLY pALD4 T-

    Plasmid
    #161497
    Purpose
    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pALD4, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161497-plasmid-map-sequence-id-326273

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  29. N229A Lo-MetQ

    Plasmid
    #118268
    Purpose
    N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Insert
    MetQ (N229A) (metQ E. coli)
    Use
    Tags
    10x His Tag and Enterokinase Cleavage Site
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala)
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    118268-plasmid-map-sequence-id-228534

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  36. pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H

    Plasmid
    #108378
    Purpose
    FRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108378-plasmid-map-sequence-id-209430

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  37. pcDNA5/FRT/TO-TPI_WT-xrRNA-4H

    Plasmid
    #108377
    Purpose
    FRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108377-plasmid-map-sequence-id-209424

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  48. GST-RP2-C86Y,P95L

    Plasmid
    #86074
    Purpose
    A bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    RP2 (RP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Changed cysteine-86 and proline-95 to tyrosine an…
    Promoter
    tac
    Available Since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    86074-plasmid-map-sequence-id-177989

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  49. CFFΔC-EGFP

    Plasmid
    #86059
    Purpose
    A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains CD8a luminal and transmembrane domains a…
    Promoter
    CMV
    Available Since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    86059-plasmid-map-sequence-id-179753

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Showing: 7301 - 7350 of 16886 results