We narrowed to 14,660 results for: flag

Showing: 5881 - 5920 of 14660 results

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pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1

Plasmid

#223717

Purpose

Expresses FLAG-TurboID-dUBA-SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

deletion 384-Stop codon

Promoter

SFFV

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA5-FRT/TO-NLS-CRBNmidi-miniTurbo-3xFLAG

Plasmid

#248675

Purpose

To generate inducible Flp-In cells expressing Cterm tagged miniTurbo CRBNmidi for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

CRBNmidi

Tags

NLS and miniTurbo-3x FLAG

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-NLS-VHL-miniTurbo-3xFLAG

Plasmid

#248670

Purpose

To generate inducible Flp-In cells expressing Cterm tagged miniTurbo full length VHL for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL (VHL Human)

Tags

FLAG-miniTurbo and NLS

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)

Plasmid

#40999

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

Type

Empty backbone

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

Plasmid

#156448

Purpose

For transient expression of human CTCF tagged with eGFP

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human CTCF (CTCF Human)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

Plasmid

#106309

Purpose

The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

Depositor

James Zhe Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)

Tags

6xflag-3xHaloTag(MtoL)

Expression

Mammalian

Promoter

EF1

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET Flag TEV LIC cloning vector (1L)

Plasmid

#29662

Depositor

Scott Gradia

Type

Empty backbone

Tags

Flag and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

Plasmid

#156440

Purpose

For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA1 (Stag1 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-MKI-MUT-P2A-GFP-DOX-N

Plasmid

#229408

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-MUT-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

interaction mutant L109G; L143G (Control mutant)

Promoter

TRE3G

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

Plasmid

#187581

Purpose

Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

COILIN (COIL Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK8-W105M-IRES-mCherry

Plasmid

#68857

Purpose

Expresses Human CDK8 W105M Mutant

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 8 (CDK8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

W105M

Promoter

EF1α

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)

Plasmid

#191172

Purpose

Human ADAR1, p150 isoform in plant-expressable format.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

3FLAG-ADAR1-p150-HIS (plant) (ADAR Human)

Tags

3FLAG and HIS

Expression

Plant

Mutation

WT

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-flag-myr-Akt1-IRES-GFP

Plasmid

#65063

Purpose

mammalian retroviral expression plasmid

Depositor

Kira Gritsman, Jean Zhao

Article

Kharas et al Blood. 2010 Feb 18;115(7):1406-15. doi:

Insert

Akt1 (AKT1 Human)

Tags

flag and myristoylation tag

Expression

Mammalian

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

Plasmid

#172620

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV UBC APEX2-3xFLAG-IRES-eGFP

Plasmid

#214983

Purpose

Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

APEX2 (APX2 Glycine max (soybean))

Use

Lentiviral

Tags

3xFLAG

Promoter

UBC

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPdU3-MND-FLAG-FLI1-IRES-GFP

Plasmid

#129496

Purpose

Lentiviral expression of human FLI1

Depositor

Marjorie Brand

Article

Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

Insert

FLI1 (FLI1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

MND

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

Plasmid

#214995

Purpose

Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-SpCas9-HF1 (without sgRNA)

Plasmid

#92102

Purpose

Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

Cbh

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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(114) pcDNA3.1-GFP-Flag-JunB-EPEA

Plasmid

#160743

Purpose

Expresses a GFP tagged JunB with Flag and EPEA tags.

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

GFP-Flag-JunB-EPEA (JUN Human)

Tags

EPEA, Flag, and GFP

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPAT

Plasmid

#187585

Purpose

Express FLAG epitope and APEX2-tagged NPAT fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NPAT (NPAT Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

Plasmid

#187574

Purpose

Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

RNPS1 (RNPS1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

Plasmid

#187576

Purpose

Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

LMNA (LMNA Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1406A

Plasmid

#169820

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1406A; TCCC -> AGTC silent mutations at nt527…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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p23-NES-EsCas13d-msfGFP-NES-Flag

Plasmid

#165074

Purpose

overexpression of EsCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

EsCas13d

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

Plasmid

#187575

Purpose

Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SRSF1 (SRSF1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

Plasmid

#90008

Purpose

Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry

Plasmid

#172640

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF3-FLAG-IRES-GFP

Plasmid

#107388

Purpose

Lentiviral expression of IKZF3-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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MMW948: CAG-DmrC-NLS-3xFLAG-VPR

Plasmid

#104565

Purpose

Mammalian expression vector for DmrC fused to VPR activator

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

DmrC-VPR

Tags

3x FLAG and NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Med24-exon minimal CMV pCDNA5

Plasmid

#212060

Purpose

LR vector for integration of Med24-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Med24-exon (Med24 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1

Plasmid

#187579

Purpose

Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

PSPC1 (PSPC1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

Plasmid

#90004

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

Plasmid

#156439

Purpose

For transient expression of mouse PDS5A tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5A (Pds5a Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
5'-3xFLAG Zmynd8+exon minimal CMV pCDNA5

Plasmid

#212079

Purpose

LR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8+exon(48nt) (Zmynd8 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

Plasmid

#90009

Purpose

Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

FKBP-linker-Myc-NBirA*
FRB-linker-CBirA*

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-IRES-Puro GFP-3xFLAG

Plasmid

#201636

Purpose

Over-expression of 3xFLAG-tagged GFP

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode

Plasmid

#170226

Purpose

Barcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

IKBKB (IKBKB Human)

Use

Lentiviral

Tags

FLAG

Mutation

S177E, S181E

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-LoxP-GFP-LoxP-SNAP-TERT

Plasmid

#71391

Purpose

Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

Depositor

Thomas Cech

Article

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Insert

TERT (TERT Human)

Use

Hr donor

Tags

FLAG-SNAP

Promoter

Endogenous TERT promotor

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 14,660 results for: flag