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Showing: 701 - 750 of 17130 results

  5. pSicoR PGK puro

    Plasmid
    #12084
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12084-plasmid-map-sequence-id-169379

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  16. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Article
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    117686-plasmid-map-sequence-id-236350

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  25. 3xFLAG-dCas9/pMSCVpuro

    Plasmid
    #134983
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134983-plasmid-map-sequence-id-430623

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  31. pLVXPuro-mGFP-LIC1

    Plasmid
    #66600
    Purpose
    Expresses Dynein-1 LIC1 as a mGFP fusion
    Depositor
    David Stephens
    Insert
    LIC1 (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  38. pCDF1-mAtf4-HT-puro

    Plasmid
    #101795
    Purpose
    Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4
    Depositor
    Seiichi Oyadomari
    Insert
    Activating transcription factor 4 (Atf4 Mouse)
    Use
    Lentiviral
    Tags
    Halo tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    101795-plasmid-map-sequence-id-194514

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  39. pMSCVsin-PGK-PURO

    Plasmid
    #35618
    Depositor
    Patrick Salmon
    Insert
    Puro
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    35618-plasmid-map-sequence-id-43780

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  42. pLEX307-ACE2-puro

    Plasmid
    #158448
    Purpose
    Constitutive lentiviral expression of human ACE2.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158448-plasmid-map-sequence-id-308163

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  48. PEA1-Nuclease-Puro

    Plasmid
    #171992
    Purpose
    Delivers all prime editing nuclease components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    171992-plasmid-map-sequence-id-338139

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Showing: 701 - 750 of 17130 results