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pAAV-TBG-ER-TurboID

Plasmid

#149415

Purpose

Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMXspuro-GFP-NBR1

Plasmid

#74202

Purpose

Retroviral expression of GFP-NBR1

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_wt_Venus_wt

Plasmid

#39813

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV mouse Dscam

Plasmid

#18737

Depositor

Joshua Sanes

Article

Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

Insert

Dscam (Dscam Mouse)

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only
hMTP

Plasmid

#124281

Purpose

Expresses human MTP in mammalian cells.

Depositor

Mahmood Hussain

Article

Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

Insert

MTTP (MTTP Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-mRuby3

Plasmid

#74252

Purpose

Mammalian expression of tandem fusion of mClover3-mRuby3

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mClover3-mRuby3

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ131-tRNA2.0

Plasmid

#158393

Purpose

Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-Blimp1

Plasmid

#73995

Purpose

Plasmid expressing mouse Blimp1

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Blimp1 gene (Prdm1 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.Cre

Plasmid

#170367

Purpose

AAV vector expressing Cre under human synapsin promotor

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET-11b_PcrA_M6_H93A

Plasmid

#194156

Purpose

Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

Depositor

Taekjip Ha

Article

Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

Insert

Engineered helicase PcrA_M6

Tags

6x His tag and FLAG tag

Expression

Bacterial

Mutation

H93A, C96A, N187C, C247A, L384V, L409C

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEff

Plasmid

#97423

Purpose

Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

Depositor

Nikolai Ravin

Article

Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ132-tRNA2.0

Plasmid

#158394

Purpose

Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. aureus scaffold CARGO array

Plasmid

#191317

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-GPI

Plasmid

#65299

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pUB-GFP

Plasmid

#11155

Purpose

Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

Ubiquitin C promoter (UBC Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pYPQ133-tRNA2.0

Plasmid

#158395

Purpose

Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ134-tRNA2.0

Plasmid

#158396

Purpose

Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2/1-CAG-Flex-FlpO-WPRE

Plasmid

#171690

Purpose

Cre-dependent expression of Flp-recombinase

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

Insert

Flip-recombinase

Use

AAV

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ135-tRNA2.0

Plasmid

#158397

Purpose

Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ136-tRNA2.0

Plasmid

#158398

Purpose

Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has Service

Cloning Grade DNA

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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mscv2.2-NLRC4

Plasmid

#60199

Purpose

Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

NLRC4 (Nlrc4 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pETM11-6xHis-TEV-hORF1p_R261A

Plasmid

#202586

Purpose

Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

ORF1p

Tags

6x His and TEV protease cleavage site

Expression

Bacterial

Mutation

Changed ORF1p Arginine 261 to Alanine

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-Caspase-4

Plasmid

#183356

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-EGFP

Plasmid

#73461

Purpose

Expresses EGFP for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

EGFP

Use

Neurotropic virus

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-GFP

Plasmid

#86537

Purpose

Retrovirus expressing GFP

Depositor

Xue Han

Article

Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

Insert

GFP

Use

Retroviral

Expression

Mammalian

Promoter

Synapsin I (human)

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-Ecadherin

Plasmid

#65286

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Tags

myc

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Plasmid

#41863

Purpose

This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti-UbC-LARGE1-EF1a-BSD

Plasmid

#205151

Purpose

Express UbC-driven LARGE1 and EF-1a driven BSD

Depositor

Monkol Lek

Insert

LARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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