We narrowed to 14,473 results for: cas9
-
Plasmid#119013PurposeMammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expressionDepositorAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
-
sg2
Plasmid#113967PurposeSingle short guide RNA targeting TACCACATTTGTAGAGGTTDepositorInsertsg2
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg3
Plasmid#113968PurposeSingle short guide RNA targeting CAATGTATCTTATCATGTCDepositorInsertsg3
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg2+sg3
Plasmid#113970PurposeDouble short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTCDepositorInsertsg2+sg3
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-hsg(NT:NT)
Plasmid#138260PurposeExpression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter systemDepositorInsertNontargeting sgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-FeGFP
Plasmid#138261PurposeDonor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmidDepositorInserteGFP
UseCRISPRExpressionMammalianPromoterNoneAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM108
Plasmid#134160PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationKMLL, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM107
Plasmid#134159PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-C3H
Plasmid#133386PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-compact
Plasmid#99369PurposeExpresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxPromoterRibi promoterAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-U61-SapI
Plasmid#112808PurposegRNA cloning vector for expressing 1-3 gRNAs in DrosophilaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR1323
Plasmid#111125PurposeExpresses non-targeting_01224 sgRNA in pCR1068DepositorInsertNon-targeting gRNA
UseLentiviralAvailable SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgPYM1
Plasmid#105249Purposehuman PYM1 sgRNADepositorInsertsgRNA for PYM1
ExpressionMammalianAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
AP1298‐1
Plasmid#87248Purposegtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWp9-2
Plasmid#73853PurposepFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1DepositorInsertUBCp-RFP-CMVp-GFP-U6p-RFP-sg1
UseLentiviralAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TS2
Plasmid#69234PurposeU6 driven SpCas9 sgRNA expression for TS2 siteDepositorInsertTS2 sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TS3
Plasmid#69235PurposeU6 driven SpCas9 sgRNA expression for TS3 siteDepositorInsertTS3 sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TS4
Plasmid#69236PurposeU6 driven SpCas9 sgRNA expression for TS4 siteDepositorInsertTS4 sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S293A
Plasmid#115204PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2
Plasmid#115199PurposeLentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S291A
Plasmid#115203PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG AsiSI/MluI
Plasmid#74286PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or MluiDepositorInsertRosa26 5-homology region
Available SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.PAC
Plasmid#57825PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter drivenDepositorInsertssgRNA
EFS
PAC
UseCRISPR and LentiviralAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP
Plasmid#57823Purpose3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP
UseCRISPR and LentiviralAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ152
Plasmid#69303PurposeGateway entry vector with pco-dCas9-VP64DepositorInsertpco-dCas9-VP64 (Plant codon-optimized)
UseCRISPRMutationVP64 activator fusion; NLS fusionAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT1T2
Plasmid#50590PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, U626t plus, U629p, T2
UseCRISPR; Pcr templateExpressionPlantAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.GFP
Plasmid#57822Purpose3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
eGFP
UseCRISPR and LentiviralAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAT009
Plasmid#171635PurposePlasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2DepositorInsertsspyCas9 sgRNA-B2M
mTagBFP2
UseCRISPR and LentiviralExpressionMammalianPromoterCAG and U6Available SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SauriBE4max
Plasmid#135969PurposeExpresses AncBE4max and nSauriCas9DepositorTypeEmpty backboneExpressionMammalianAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJCHph
Plasmid#185863PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1
Plasmid#119007PurposeMammalian expression of human BRCA1 and BFPDepositorAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP657
Plasmid#57824PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP657
UseCRISPR and LentiviralAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX552
Plasmid#60958PurposepAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.DepositorInsertsU6_(SpaI)_sgRNA
Syn_EGFP-KASH
UseAAV, CRISPR, and Mouse TargetingExpressionMammalianPromoterSynapsin and U6Available SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-tagBFP-PSM-dTOMATO
Plasmid#100604PurposeDonor scafold with dTOMATO positive selection module, and tagBFP negative selection moduleDepositorTypeEmpty backboneUseSynthetic BiologyTagsdTOMATO-T2A-Puro and tagBFPAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-tgRNA-Rev
Plasmid#112811PurposegRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in DrosophilaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGC
Plasmid#112812PurposePCR template vector for amplifying gRNA(F+E)-OstRNA fragmentDepositorInsertgRNAcore(F+E)-tRNA
ExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-NLS-tdMCP-mCherry
Plasmid#99909PurposeFor the expression of NLS-tdMCP-mCherryDepositorInsertNLS-tdMCP-mCherry
UseLentiviralExpressionMammalianPromoterTRE3GAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-Cetn1
Plasmid#50717PurposeCetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.DepositorAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/GFP Dest
Plasmid#74281PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseMouse TargetingAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/GFP Asc
Plasmid#74285PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscIDepositorInsertRosa26 5-homology region
UseMouse TargetingAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only