Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,170 results

Showing: 7601 - 7650 of 177170 results

  3. FLX1.8NFATGFPpd2HS4

    Plasmid
    #169096
    Purpose
    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation
    Depositor
    Geoffrey Owens
    Inserts
    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    Use
    Lentiviral
    Promoter
    NFAT enhancer + synthetic TATA box
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169096-plasmid-map-sequence-id-338033

    Enlarge

  4. Flag-M4-AR

    Plasmid
    #171240
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)
    Depositor
    Steven Balk
    Publication
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-tagged human androgen receptor (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    171240-plasmid-map-sequence-id-336015

    Enlarge

  5. pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

    Plasmid
    #162280
    Purpose
    Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Publication
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SCN8A Variant 1, stabilized (SCN8A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    Modified human HSPA5 intron 4 inserted after bp23…
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    162280-plasmid-map-sequence-id-334621

    Enlarge

  6. pC1-HyPerRed-mito

    Plasmid
    #60247
    Purpose
    Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.
    Depositor
    Vsevolod Belousov
    Publication
    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyPerRed-mito
    Tags
    Mitochondrial targeting signal
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    60247-plasmid-map-sequence-id-317302

    Enlarge

  8. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid
    #159107
    Purpose
    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).
    Depositor
    Thomas Tuschl
    Publication
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    NSP12
    Tags
    His-SUMO tag
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    159107-plasmid-map-sequence-id-310025

    Enlarge

  11. pCX-SpiCee

    Plasmid
    #140836
    Purpose
    mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling
    Depositor
    Xavier Nicol
    Publication
    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpiCee
    Tags
    mRFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    140836-plasmid-map-sequence-id-304813

    Enlarge

  14. pCMV-2E2-HA-mEGFP

    Plasmid
    #129595
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Publication
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129595-plasmid-map-sequence-id-254267

    Enlarge

  19. ARHGEF11(DHPH)-CRY2-mCherry

    Plasmid
    #89481
    Purpose
    Also known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11
    Depositor
    Xavier Trepat Guixer
    Publication
    Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ARHGEF11(DHPH) - CRY2(1-498) - mCherry (CRY2, ARHGEF11 Human, Mustard Weed)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    89481-plasmid-map-sequence-id-183280

    Enlarge

  25. pENN.AAV.CamKII 0.4.Cre.SV40

    Plasmid
    #105558
    Purpose
    AAV expression of Cre from CamKII promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CamKII
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92 Articles
    105558-plasmid-map-sequence-id-202399

    Enlarge

  27. Opto-CRAC version 1

    Plasmid
    #101245
    Purpose
    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling
    Depositor
    Yubin Zhou
    Publication
    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LOV2 and STIM1 cytosolic fragment
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101245-plasmid-map-sequence-id-209272

    Enlarge

  28. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Publication
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    98592-plasmid-map-sequence-id-201329

    Enlarge

  29. pENN.AAV.CMVs.Pl.Cre.rBG

    Plasmid
    #105537
    Purpose
    AAV expression of Cre from CMV promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, and AAV rh10
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    105537-plasmid-map-sequence-id-202428

    Enlarge

  40. pcDNA3_erGAP3

    Plasmid
    #78118
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Depositor
    Teresa Alonso, Javier García-Sancho
    Publication
    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    erGAP3
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    S175G, D180Y, I167V and L15Q in GFP moiety and D…
    Promoter
    CMV
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    78118-plasmid-map-sequence-id-153361

    Enlarge

  43. NLS-mCherry-NES reporter (pDN160)

    Plasmid
    #72660
    Purpose
    NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158
    Depositor
    Barbara Di Ventura, Roland Eils
    Publication
    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-mCherry-NES
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    72660-plasmid-map-sequence-id-135490

    Enlarge

  44. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid
    #72325
    Purpose
    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.
    Depositor
    Mark Howarth
    Publication
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-mEGFP-SpyTag
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    72325-plasmid-map-sequence-id-137259

    Enlarge

  49. pcDNA5FRT-EF-Pdgfrbeta-EGFPN

    Plasmid
    #66790
    Purpose
    Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter
    Depositor
    Lotte Pedersen
    Publication
    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PDGFRbeta (Pdgfrb Mouse)
    Tags
    C-term EGFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    66790-plasmid-map-sequence-id-121938

    Enlarge

  50. Str-KDEL_neomycin

    Plasmid
    #65306
    Purpose
    Luminal ER hook only (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    Tags
    KDEL motif and Signal Sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    65306-plasmid-map-sequence-id-110514

    Enlarge

Showing: 7601 - 7650 of 177170 results