We narrowed to 7,508 results for: PAC

1. 36INS-pTet_GB

   Plasmid

   #78681

   Purpose

   Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [G:36INS-pTet:B]

   Depositor

   Douglas Densmore

   Article

   Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

   Insert

   Insulated pTet Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCas8

   Plasmid

   #82380

   Purpose

   dCas9 without a promoter in acsA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 36INS-pTet_FB

   Plasmid

   #78680

   Purpose

   Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [F:36INS-pTet:B]

   Depositor

   Douglas Densmore

   Article

   Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

   Insert

   Insulated pTet Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 36INS-pTet_EB

   Plasmid

   #78679

   Purpose

   Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [E:36INS-pTet:B]

   Depositor

   Douglas Densmore

   Article

   Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

   Insert

   Insulated pTet Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 36INS-pTet_AB

   Plasmid

   #78678

   Purpose

   Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [A:36INS-pTet:B]

   Depositor

   Douglas Densmore

   Article

   Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

   Insert

   Insulated pTet Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SAH0141-0.0-conP01

   Plasmid

   #79828

   Purpose

   Transcriptional interference assessment; strong interfering promoter, landing site for spacers

   Depositor

   Katja Arndt

   Article

   Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

   Inserts

   Blue fluorescent protein
   mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   bla P3 and conP01

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 36INS1-pBAD_GB

   Plasmid

   #78673

   Purpose

   Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [G:36INS1-pBAD:B]

   Depositor

   Douglas Densmore

   Article

   Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

   Insert

   Insulated pBAD Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 36INS1-pBAD_FB

   Plasmid

   #78672

   Purpose

   Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [F:36INS1-pBAD:B]

   Depositor

   Douglas Densmore

   Article

   Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

   Insert

   Insulated pBAD Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 36INS1-pBAD_EB

   Plasmid

   #78671

   Purpose

   Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. [E:36INS1-pBAD:B]

   Depositor

   Douglas Densmore

   Article

   Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

   Insert

   Insulated pBAD Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SAH0146-0.5-conP01

    Plasmid

    #79831

    Purpose

    Transcriptional interference assessment; strong interfering promoter, 500 bp spacer

    Depositor

    Katja Arndt

    Article

    Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

    Inserts

    Blue fluorescent protein
    mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    bla P3 and conP01

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pArk313

    Plasmid

    #200257

    Purpose

    Ark313 rep/cap

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    Ark313 Rep/Cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Fuw-dCas9-Tet1CD

    Plasmid

    #84475

    Purpose

    Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMSP1D1

    Plasmid

    #20061

    Purpose

    Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    deletion mutant (1-11) of MSP1; His-tag on N-term…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FU-tetO-Gateway

    Plasmid

    #43914

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-TRE-Tight2-AkaLuc

    Plasmid

    #186186

    Purpose

    AAV vector packaging AkaLuciferase under the TRE promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

    Insert

    AkaLuc luciferase
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE-Tight2

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRepCap6

    Plasmid

    #110770

    Purpose

    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.

    Depositor

    David Russell

    Article

    Rutledge et al J Virol. 1998 Jan;72(1):309-19.

    Insert

    AAV6 cap, AAV6 rep genes
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CAP-AAV.FUS.3

    Plasmid

    #222333

    Purpose

    AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

    Depositor

    Mikhail Shapiro, Jerzy Szablowski

    Article

    Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

    Insert

    AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    p41

    Available since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. T7-VEE-IRES-Puro

    Plasmid

    #58970

    Purpose

    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Type

    Empty backbone

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Fuw-dCas9-Tet1CD_IM

    Plasmid

    #84479

    Purpose

    Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD_IM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti_C_Myc_DDK_IRES_Puro_PRKRA

    Plasmid

    #106113

    Purpose

    Lentiviral expression of PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Lentiviral

    Tags

    DDK (FLAG) and Myc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET264-pUC 24xMS2V6 Loxp KANr Loxp

    Plasmid

    #104393

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Fuw-dCas9-Dnmt3a_IM

    Plasmid

    #84478

    Purpose

    Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a_IM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TTVTFLPTPSLILQT_epitope

    Plasmid

    #165021

    Purpose

    Expression of CMV UL44 (38-52) fused to CD74

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV UL44 (38-52)
    

    Use

    Lentiviral

    Tags

    CD74 at N-term; cathepsin S cleavage site (GRWHTV…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAD-IRP

    Plasmid

    #220751

    Purpose

    Plasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Insert

    Iron Regulatory Protein (ACO1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDGM3B

    Plasmid

    #110809

    Purpose

    contains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genes

    Depositor

    David Russell

    Article

    Khan et al Nat Protoc. 2011 Apr;6(4):482-501. doi:

    Insert

    AAV3B cap genes, AAV2 rep genes, adenovirus helper genes
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDMS151A_Tet-On-3G

    Plasmid

    #191577

    Purpose

    ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    Tet-On(R) 3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMIIS638

    Plasmid

    #238210

    Purpose

    Expresses native SpTasH TIGR locus. CmR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    SpTasH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMIIS644

    Plasmid

    #238209

    Purpose

    Expresses native FpTasA TIGR locus. CmR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    FpTasA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDonor14-AcHELIX-KanR (CJT96)

    Plasmid

    #181794

    Purpose

    pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBR322-Pdp1_NTD-GFP-HiBiT

    Plasmid

    #198317

    Purpose

    Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-GFP-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMT-flp

    Plasmid

    #67274

    Purpose

    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

    Depositor

    Mark Liles

    Article

    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

    Insert

    tetA, flp, repA101, oriR101
    

    Use

    Flp recombinase plasmid, temperature sensitive

    Tags

    His-SUMO (flp)

    Expression

    Mutation

    Promoter

    tetA promoter of plasmid pACYC184 origin

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLG027 (qatABCD)

    Plasmid

    #157905

    Purpose

    Expresses the qatABCD defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    qatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LZRS-hNEDD9_IresGFP

    Plasmid

    #21962

    Depositor

    Lynda Chin

    Article

    Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

    Insert

    NEDD9 (NEDD9 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2010

    Availability

    Academic Institutions and Nonprofits only

37. Adeno Cas9

    Plasmid

    #64072

    Purpose

    Adenoviral vector for the expression of the Cas9

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    U6 and CBh

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET251-pUC 12xMS2V6 Loxp KANr Loxp

    Plasmid

    #104392

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDIV643

    Plasmid

    #226182

    Purpose

    Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    uidA marker flanked with targeting regions to KU70 gene in K.phaffii
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TEF1p

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MD-2

    Plasmid

    #20864

    Depositor

    Jie-Oh Lee

    Article

    Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

    Insert

    Myeloid differentiation factor 2 (LY96 Human)
    

    Use

    Tags

    protein A tag

    Expression

    Insect

    Mutation

    contains amino acids 19–160

    Promoter

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge