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20,193 results

Showing: 601 - 640 of 20193 results

  9. pDAC446

    Plasmid
    #195239
    Purpose
    Mammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195239-plasmid-map-sequence-id-382947

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  12. pSpot-Tag-GFP-GPI

    Plasmid
    #117061
    Purpose
    For mammalian expression of GPI-anchored GFP with N-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    GPI-anchored GFP
    Tags
    GPI-anchor and Spot-Tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117061-plasmid-map-sequence-id-224390

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  18. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102886
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102886-plasmid-map-sequence-id-194712

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  28. AICSDP-25:MAP1LC3B-mEGFP

    Plasmid
    #101783
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B
    Depositor
    Allen Institute for Cell Science
    Insert
    MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101783-plasmid-map-sequence-id-197671

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  30. pQE80L-IL2 C125S

    Plasmid
    #104348
    Purpose
    Expresses human IL-2 (C125S)
    Depositor
    Alexander McLellan
    Insert
    Human IL-2 (C125S) (IL2 Human)
    Tags
    MRGSHHHHHGS-tag
    Expression
    Bacterial
    Mutation
    C125S mutation (TGT -> TCC)
    Promoter
    T5
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104348-plasmid-map-sequence-id-201216

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  35. TrHKII-pGFPN3

    Plasmid
    #21921
    Depositor
    Hossein Ardehali
    Article
    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.
    Insert
    Truncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid (HK2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This is the truncated human HKII cDNA sequence (l…
    Available Since
    Oct. 1, 2009
    Availability
    Academic Institutions and Nonprofits only

  36. pJZC74

    Plasmid
    #62342
    Purpose
    sgRNA + 1x COM with COM-KRAB effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 1x COM binding module
    COM-KRAB
    Use
    Lentiviral
    Tags
    KRAB
    Expression
    Mammalian
    Mutation
    Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…
    Promoter
    CMV and U6
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62342-plasmid-map-sequence-id-102265

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  40. pJZC116

    Plasmid
    #62344
    Purpose
    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP
    Depositor
    Wendell Lim, Stanley Qi
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 2x MS2 (wt+f6) binding module
    MCP-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGC
    Promoter
    CMV and U6
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62344-plasmid-map-sequence-id-102267

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Showing: 601 - 640 of 20193 results