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9,049 results

Showing: 7951 - 8000 of 9049 results

  3. pEGFP-mNFM

    Plasmid
    #32909
    Purpose
    Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein M
    Depositor
    Anthony Brown
    Article
    Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):
    Insert
    EGFP-mouse NFM fusion (Nefm Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32909-plasmid-map-sequence-id-38896

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  11. MuHKII-pGFPN3

    Plasmid
    #21922
    Depositor
    Hossein Ardehali
    Article
    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.
    Insert
    Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This is the full length human HKII cDNA sequence …
    Available Since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only

  12. pGEX-2T-Rac1-wt

    Plasmid
    #12977
    Depositor
    Gary Bokoch
    Insert
    Rac1 (RAC1 Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  14. 1435 pSG5L Flag HA

    Plasmid
    #10791
    Purpose
    Mammalian expression empty backbone.
    Depositor
    William Sellers
    Type
    Empty backbone
    Tags
    Flag and HA
    Expression
    Mammalian
    Available Since
    Sept. 28, 2005
    Availability
    Academic Institutions and Nonprofits only
    10791-plasmid-map-sequence-id-482206

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  20. GABA(A) receptor subunit a2SE

    Plasmid
    #49169
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    Tags
    HA, bovine alpha 1 signal sequence, and pHGFP (pH…
    Expression
    Mammalian
    Mutation
    T343A
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49169-plasmid-map-sequence-id-72495

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  36. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid
    #40132
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease (ELD)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
    40132-plasmid-map-sequence-id-51020

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  42. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65771-plasmid-map-sequence-id-112653

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  45. pEGFP-ATF6-(S1P-)

    Plasmid
    #32956
    Purpose
    For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.
    Depositor
    Ron Prywes
    Article
    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-
    Insert
    ATF6 (ATF6 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S1Protease site mutation: R415A/R416A
    Promoter
    CMV
    Available Since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    32956-plasmid-map-sequence-id-226826

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  47. GANLS

    Plasmid
    #50852
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. CAG-Flex-TC66T

    Plasmid
    #48331
    Purpose
    Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA
    Depositor
    Liqun Luo
    Article
    Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1
    Insert
    TVA receptor mCherry fusion with the 66T mutation
    Use
    AAV and Cre/Lox
    Tags
    mCherry fusion to TVA receptor
    Expression
    Mammalian
    Mutation
    66T
    Promoter
    CAG
    Available Since
    Dec. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    48331-plasmid-map-sequence-id-130617

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Showing: 7951 - 8000 of 9049 results