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8,883 results

Showing: 7951 - 8000 of 8883 results

  6. pMLM3705

    Plasmid
    #47754
    Purpose
    Expresses mammalian cell codon-optimized dCas9-VP64
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Insert
    codon optimized dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9 (catalytically i…
    Promoter
    CMV
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47754-plasmid-map-sequence-id-68222

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  14. pcDNA3-HA-ADH

    Plasmid
    #11610
    Depositor
    Steven Johnson
    Insert
    aldehyde dehydrogenase (ALDH1A1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K412I
    Available Since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only
    11610-plasmid-map-sequence-id-232087

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  29. pcDNA3-T7-ERK1

    Plasmid
    #14440
    Depositor
    John Blenis
    Insert
    ERK1 (Mapk3 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Available Since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    14440-plasmid-map-sequence-id-254030

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  30. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid
    #35498
    Purpose
    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    Ef1a
    Available Since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35498-plasmid-map-sequence-id-199355

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  42. pRSET.GltI253-cpGFP.L1LV/L2NP

    Plasmid
    #41733
    Purpose
    a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Bacterial
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    T7
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41733-plasmid-map-sequence-id-56305

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  43. pCS2TAL3-DD

    Plasmid
    #37275
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    Flag, Fok I DD, Tal-C, and Tal-N
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37275-plasmid-map-sequence-id-46872

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  45. pCGN-ATF6 (1-373)m1

    Plasmid
    #27174
    Purpose
    For mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.
    Depositor
    Ron Prywes
    Article
    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2
    Insert
    ATF6 (ATF6 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Contains only aa 1-373 Amino acids 315–317 are c…
    Available Since
    Jan. 5, 2011
    Availability
    Academic Institutions and Nonprofits only

  47. pCS2TAL3-RR

    Plasmid
    #37276
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37276-plasmid-map-sequence-id-46873

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Showing: 7951 - 8000 of 8883 results