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We narrowed to 18,759 results for: nls

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Showing: 6441 - 6480 of 18759 results

  3. pEf1a-superDn29-dCas9-P2A-GFP

    Plasmid
    #247156
    Purpose
    Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    superDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…
    Promoter
    Ef1a
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247156-plasmid-map-sequence-id-491689

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  4. ABE-NW1

    Plasmid
    #240615
    Purpose
    Adenine base editor with narrowed editing window
    Depositor
    Tingting Jiang
    Publication
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA8e(N108Q,M151C,Q154Y)-linker-nCas9 (D10A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    TadA8e(N108Q,M151C,Q154Y),Cas9 (D10A)
    Available since
    July 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240615-plasmid-map-sequence-id-480856

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  5. pIF1079

    Plasmid
    #237445
    Purpose
    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.
    Depositor
    IIya Finkelstein
    Publication
    Buffington et al Nat Biotechnol. 2025 Oct 23. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CAG, H1, and U6
    Available since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    237445-plasmid-map-sequence-id-476973

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  10. gag-TadCBEd

    Plasmid
    #225144
    Purpose
    Packaging plasmid for TadCBEd in v3 PE-eVLP architecture
    Depositor
    David Liu
    Publication
    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-TadCBEd
    Expression
    Mammalian
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    225144-plasmid-map-sequence-id-445269

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  12. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Publication
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  13. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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  16. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

    Plasmid
    #209080
    Purpose
    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression
    Depositor
    Vikram Khurana
    Publication
    Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    A53T
    Promoter
    TRE4G
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209080-plasmid-map-sequence-id-416548

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  17. 4X PRE TK luc

    Plasmid
    #206159
    Purpose
    Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350
    Depositor
    Renee van Amerongen
    Publication
    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4X PRE TK luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Available since
    Nov. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    206159-plasmid-map-sequence-id-414736

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  20. pAf-CRISPR-yA

    Plasmid
    #191015
    Purpose
    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.
    Depositor
    Perng-Kuang Chang
    Publication
    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    yA
    Use
    CRISPR
    Promoter
    Aspergillus flavus U6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    191015-plasmid-map-sequence-id-516753

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  22. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

    Plasmid
    #188383
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor
    Depositor
    Kole Roybal
    Publication
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
    Use
    Lentiviral
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188383-plasmid-map-sequence-id-371054

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  23. CABE-T3.1

    Plasmid
    #193267
    Purpose
    Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter
    Depositor
    Nicole Gaudelli
    Publication
    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CABE-T3.1
    Expression
    Mammalian
    Mutation
    D10A (Cas9), TadA mutations within
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193267-plasmid-map-sequence-id-379830

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  26. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid
    #187952
    Purpose
    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-CasRx-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker, HA-tag, and NLS
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187952-plasmid-map-sequence-id-373488

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  30. pX459-sgAAVS1

    Plasmid
    #184403
    Purpose
    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT
    Depositor
    Robert Bradley
    Publication
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-specific sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184403-plasmid-map-sequence-id-362381

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  31. pdCas9-GFP-VPR

    Plasmid
    #181907
    Purpose
    Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta
    Depositor
    Karsten Rippe
    Publication
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181907-plasmid-map-sequence-id-359690

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  40. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid
    #129025
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129025-plasmid-map-sequence-id-454365

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Showing: 6441 - 6480 of 18759 results