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Plasmid#119155PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
1137=Hsp70Bb-Cas9[dsRed]
Plasmid#149424PurposeThe piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)DepositorInsertCas9
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-GFP-Granuphilin
Plasmid#170867PurposeMammalian expression of His-GFP-Granuphilin.DepositorInsertSynaptotagmin-like 4 (SYTL4 Human)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-2A-oG-WPRE
Plasmid#102984PurposeLentivirus expressing eGFP and optimised Rabies Glycoprotein (oG)DepositorInsertsEnhanced Green Fluorescent Protein (eGFP)
Optimised Rabies glycoprotein (oG)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS2A
Plasmid#145343PurposeCell-free/mammalian expression vector for Zika virus NS2A protein with N-term eGFPDepositorInsertZIKV NS2A
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceFeb. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKVC
Plasmid#145339PurposeCell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFPDepositorInsertZIKV Capsid
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceApril 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV plasmid
Plasmid#169739PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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3922-48
Plasmid#176497PurposepTRE-EGFP-T2A-TFEB(wt)-polyADepositorInsertEGFP-T2A-TFEB(wild-type; full-length; human)
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M4NS_AmpR
Plasmid#119156PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC15-P1B3
Plasmid#202856PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC15DepositorInsertEGFP/BC15
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC11-P1A11
Plasmid#202852PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC11DepositorInsertEGFP/BC11
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBAC690-TEF
Plasmid#160432PurposeExpresses eGFP under control of the yeast TEF2 promoterDepositorInsertS. cerevisiae TEF2 promoter
UseTagsExpressionYeastMutationPromoterS. cerevisiae TEF2Available sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKF-P14MM2AG5h
Plasmid#128254PurposeMammalian positive feedback synthetic gene circuit within a Flp-In expression vector.DepositorInsertrtTA-Advanced::P2A::EGFP
UseSynthetic Biology; Flp-in expression vectorTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron-G
Plasmid#40027DepositorInsertsGFP exon 1
GFP exon 2
beta-globin intron
UseTagsExpressionMammalianMutationinsertion of a loxP site, nucleotides 1-274, and …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
Abp1-tDimerRFP
Plasmid#27699DepositorInsertAbp1 (Dbnl Mouse)
UseTagstDimer-RFPExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
LZRS-GATA3
Plasmid#34836DepositorInsertGATA3 (Gata3 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutationPromoterLTRAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2A-EKAREV-NLS
Plasmid#173856PurposeEncoding EKAREV-NLS.DepositorInsertEKAREV-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC82-P1G10
Plasmid#202923PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82DepositorInsertEGFP/BC82
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS2B
Plasmid#145344PurposeCell-free/ mammalian expression vector for Zika virus NS2B protein with N-term eGFPDepositorInsertZIKV NS2B
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS4B
Plasmid#145347PurposeCell-free/ mammalian expression vector for Zika virus NS4B protein with N-term eGFPDepositorInsertZIKV NS4B
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
3924-55
Plasmid#176499PurposepTRE-EGFP-T2A-TFEB(S3E)-polyADepositorInsertEGFP-T2A-TFEB(human; full-length; S3E mutation)-
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
3923-51
Plasmid#176498PurposepTRE-EGFP-T2A-TFEB(S3A)-polyADepositorInsertEGFP-T2A-TFEB(human; full-length; S3A mutation)
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL7Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC2-P1A2
Plasmid#202843PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2DepositorInsertEGFP/BC2
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC6-P1A6
Plasmid#202847PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6DepositorInsertEGFP/BC6
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Beta-arrestin2 K11,12R GFP
Plasmid#35412DepositorInsertβarrestin2 (Arrb2 Rat)
UseTagsEGFPExpressionMammalianMutationK11,12RPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
FH95pUp95GW (B4)
Plasmid#74013Purposelentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNADepositorInsertPsd95 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET/LEGH
Plasmid#108226PurposeBacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tagDepositorInsertLBT-EGFP-His6
UseTagsExpressionBacterialMutationPromoterT7, lacOAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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A3D-Cas9n-UGI
Plasmid#119137PurposeBase editor made from APOBEC3DDepositorInsertAPOBEC3D-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS1
Plasmid#145342PurposeCell-free/mammalian expression vector for Zika virus NS1 protein with N-term eGFPDepositorInsertZIKV NS1
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
A3F-Cas9n-UGI
Plasmid#119138PurposeBase editor made from APOBEC3FDepositorInsertAPOBEC3F-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
A3C-Cas9n-UGI
Plasmid#119136PurposeBase editor made from APOBEC3CDepositorInsertAPOBEC3C-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M7NS_AmpR
Plasmid#119157PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M2S_AmpR
Plasmid#119154PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
phNFL-Q332P
Plasmid#132599PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutationDepositorInsertNefl (Nefl Human)
UseTagsExpressionMammalianMutation995A>C;Gln332ProPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3
Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP reporterExpressionYeastMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneUseTagsyEGFP-CLN2PEST reporterExpressionYeastMutationPromoterAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pmCellFree_KA1_ZIKV PrM
Plasmid#145340PurposeCell-free/mammalian expression vector for Zika virus PrM protein with N-term eGFPDepositorInsertZIKV PrM
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceApril 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS3
Plasmid#145345PurposeCell-free/ mammalian expression vector for Zika virus NS3 protein with N-term eGFPDepositorInsertZIKV NS3
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceApril 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS4A
Plasmid#145346PurposeCell-free/mammalian expression vector for Zika virus NS4A protein with N-term eGFPDepositorInsertZIKV NS4A
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceApril 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
A3G-Cas9n-UGI
Plasmid#119139PurposeBase editor made from APOBEC3GDepositorInsertAPOBEC3G-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationNonePromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-5
Plasmid#160705PurposeTrichoderma optimised Synthetic eGFP bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRL WS1.6 GFP
Plasmid#36249DepositorInsertGFP (eGFP )
UseLentiviralTagsExpressionMutationPromoterWAS (human 1.6 kb)Available sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only