We narrowed to 22,820 results for: Fus

1. pTD-NTwinStrep_Sm

   Plasmid

   #45937

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19/ER-mChe

   Plasmid

   #183163

   Purpose

   Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   ER-mCherry
   

   Use

   Tags

   25 aa ER signal of Glycine soja kunitz-type tryps…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pF(UG) hSyn SYP-HaloTag

   Plasmid

   #188907

   Purpose

   Expresses synaptic HaloTag

   Depositor

   Edwin Chapman

   Article

   Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

   Insert

   SYP (Syp Mouse)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19/mChe-PM

   Plasmid

   #183168

   Purpose

   Construct for transient expression of plasma membrane-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry-PM
   

   Use

   Tags

   OsRac3, O. sativa rac-like GTP-binding protein 3

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBMN PLEKHM1-HA

   Plasmid

   #89300

   Purpose

   For expression of PLEKHM1 tagged with HA

   Depositor

   Michael Lazarou

   Article

   Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

   Insert

   PLEKHM1 (PLEKHM1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2 GFP-GSK3-MAPK

   Plasmid

   #29689

   Purpose

   GFP reporter protein fused to three GSK3 phosphorylation sites

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   GFP glycogen synthase kinase 3 recognition site primed by MAPK
   

   Use

   Tags

   Flag and Flag-EGFP

   Expression

   Mammalian

   Mutation

   recognition sites only, and E3 ligase site

   Promoter

   SP6

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJL-SaKKH-BE3

   Plasmid

   #85170

   Purpose

   Expresses SaKKH-BE3 in mammalian cells [Edit]

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   SaKKH-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19/NLS-mChe

   Plasmid

   #183162

   Purpose

   Construct for transient expression of cell nucleus-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   NLS-mCherry
   

   Use

   Tags

   16 aa nuclear localization signal and 16 aa nucl…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXsIP GFP-VPS33A

   Plasmid

   #67022

   Purpose

   human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector

   Depositor

   Noboru Mizushima

   Article

   Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

   Insert

   Vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pFX-EGFP-Rab7

    Plasmid

    #174457

    Purpose

    Express EGFP with late endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with late endosome-targeting sequence
    

    Use

    Tags

    small GTPase (Rab7)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET24a-LaM4-EGFP

    Plasmid

    #182641

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, EGFP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. (112) pcDNA3.1-GFP-Flag-Nup62-EPEA

    Plasmid

    #168101

    Purpose

    GFP and Flag tagged Nup62 with EPEA nanobody tag

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    GFP-Flag-Nup62-EPEA
    

    Use

    Tags

    EPEA, Flag, and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-dCas9-MQ1(WT)-EGFP

    Plasmid

    #89633

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19/Mito-mChe

    Plasmid

    #183167

    Purpose

    Construct for transient expression of mitochondrion-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    Mito-mCherry
    

    Use

    Tags

    OsCOX11, Oryza sativa cytochrome c oxidase assemb…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFX-EGFP-Rab5

    Plasmid

    #174454

    Purpose

    Express EGFP with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with early endosome-targeting sequence
    

    Use

    Tags

    small GTPase (Rab5)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2 GFP-GSK3mut-MAPK

    Plasmid

    #29690

    Purpose

    GFP reporter protein fused to three mutated GSK3 phosphorylation sites

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
    

    Use

    Tags

    Flag and Flag-EGFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site, GSK3 …

    Promoter

    SP6

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQCXIP-cathepsinD-YMH

    Plasmid

    #182644

    Purpose

    Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    cathepsinD
    

    Use

    Retroviral

    Tags

    His6-tag, TS site from proCCK, and myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. (294) pcDNA3.1-myc-OGA(1-400)

    Plasmid

    #168095

    Purpose

    Myc tagged truncated human OGA catalytic domain of residues 1-400

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    myc-OGA(1-400)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTD-NTwinStrep_Km

    Plasmid

    #45938

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBECKP-spe

    Plasmid

    #117236

    Purpose

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXPR_206

    Plasmid

    #96920

    Purpose

    for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-AF8

    Plasmid

    #117803

    Purpose

    mammalian expression of GFP-tagged human centromere protein A

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2000 Aug;25(4):253-61

    Insert

    CENP-A (CENPA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ABI_cTEVp

    Plasmid

    #119214

    Purpose

    Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cTEVp in fusion with ABI
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBK-VQR-BE3

    Plasmid

    #85171

    Purpose

    Expresses VQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    VQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-DD-T6B-MUT-YFP

    Plasmid

    #235144

    Purpose

    Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    DHFR-fused mutant T6B peptide
    

    Use

    AAV

    Tags

    Expression

    Mutation

    5 tryptophan residues of T6B-WT mutated

    Promoter

    hSyn

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-P4:gs:nls:CIBN

    Plasmid

    #135986

    Purpose

    Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    P4 coiled-coil fused to the CIBN domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid

    #115775

    Purpose

    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    

    Use

    Tags

    EGFP; dsReD

    Expression

    Mammalian

    Mutation

    gp140 uncleaved (1190-91aa, KR > TG; truncated…

    Promoter

    HIV-1 (LTR)

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFastBac HT JS-Munc18c

    Plasmid

    #135555

    Purpose

    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18c
    

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

    Plasmid

    #121184

    Purpose

    OsTIR1 introduction

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    CMV-OsTIR1-loxP-PURO-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    OsTIR1 codon optimized for human cells

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-CD63-(TEV)-tTA

    Plasmid

    #162597

    Purpose

    human CD63-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD63 (CD63 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFX-mCherry-KRasCT

    Plasmid

    #175835

    Purpose

    Express mCherry with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with plasma membrane-targeting sequence
    

    Use

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSL3402 (pTnsABf_Pse)

    Plasmid

    #200901

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsA-TnsB
    

    Use

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-dCpf1-BE

    Plasmid

    #107685

    Purpose

    Expresses dLbCpf1-BE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D832A/E925A/D1148A

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-28a-His6-SUMO-Munc18-1wt

    Plasmid

    #135550

    Purpose

    Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18-1wt
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFX-β-tubulin-EGFP

    Plasmid

    #175827

    Purpose

    Express EGFP with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with microtuble-targeting sequence
    

    Use

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. plko-shmfn2-mito-GFP-ER tethering

    Plasmid

    #160509

    Purpose

    Rescue mfn2 knockdown by tethering mitochondria and ER with GFP

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    mito-GFP-ER tethering
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBSV2G_P0826-BB0323-mCherryBb

    Plasmid

    #118244

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    BB0323-mCherry translational fusion under the control of the bb0826 promoter
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFX-mKate2-Rab11

    Plasmid

    #174463

    Purpose

    Express ｍKate2 with recycling endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mKate2 with recycling endosome-targeting sequence
    

    Use

    Tags

    small GTPase (Rab11)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. A3A-PBE

    Plasmid

    #119768

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A and E945K in wild type …

    Promoter

    maize Ubiquitin-1

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ECFP-GalT

    Plasmid

    #11937

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUASTattB-dALFANb(3mut)-mGreenLantern

    Plasmid

    #219662

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMXs-Lhx3

    Plasmid

    #32927

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Lhx3 (Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

43. pcDNA3.1-CD81-(TEV)-Cre

    Plasmid

    #167939

    Purpose

    human CD81-TEV cleavage site-Cre

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD81 (CD81 Human)
    

    Use

    Tags

    Cre recombinase and TEV protease cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pMXs-Ngn2

    Plasmid

    #32928

    Purpose

    Retroviral expression of mouse Ngn2

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Ngn2 (Neurog2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

45. MMW948: CAG-DmrC-NLS-3xFLAG-VPR

    Plasmid

    #104565

    Purpose

    Mammalian expression vector for DmrC fused to VPR activator

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    DmrC-VPR
    

    Use

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pMXs-Isl1

    Plasmid

    #32929

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Isl1 (Isl1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

47. pFX-mito-eqFP650

    Plasmid

    #174179

    Purpose

    Express eqFP650 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    eqFP650 with mitochondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. OPA1-isoform2

    Plasmid

    #70174

    Purpose

    Mammalian expression of hsOPA1 isoform 2

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 2 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBK-YE1-BE3

    Plasmid

    #85174

    Purpose

    Expresses YE1-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YE1-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST

    Plasmid

    #175019

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    D/bovine/Nebraska/9-5/2012
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge