We narrowed to 30,195 results for: REP
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Plasmid#140299PurposeExpresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X FlagExpressionMammalianMutationlacks the UD regionPromoterCMVAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIE-TGFB2-H6
Plasmid#128500PurposeExpresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.DepositorAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR
Plasmid#136514PurposeExpresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vectorDepositorInsertCodon-optimized AcrIIA5
UseLentiviralTagsFLAG/NLSExpressionMammalianPromoterSFFVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV5-B
Plasmid#74978PurposeshETV5 shRNADepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-Cas9-V5
Plasmid#87905PurposeCloning vectorDepositorInsertCas9-V5
UseCloning vectorTagsV5MutationHuman codon optimizedPromoterV5Available SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.5 shETV5-A
Plasmid#74977PurposeshETV5 shRNADepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-hGH
Plasmid#182411PurposeBacterial expression of Vesicle Nucleating peptide-human growth hormone fusionDepositorAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-iChR2++
Plasmid#98172Purposeartificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChR2++ gene
TagsmCherryExpressionMammalianMutationA59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…PromoterCMV (+enhancer)Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDest-636
Plasmid#159574PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneTagsHis6-MBPExpressionInsectAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FullLength-TET1-CXXC(24)
Plasmid#124398PurposeFull-length TET1 missing zinc finger domain (585-624) used to PiggyBAC rescue the function of TET1/2 DKO cellsDepositorInsertFLAG-FullLength-TET1 with CXXC zinc finger deletion (585-624) (TET1 Human)
TagsFlagExpressionMammalianMutationCXXC zinc finger deletion (aa 585-624)Available SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3
Plasmid#87906PurposesgRNA targeting GFPDepositorInsertsgGFP1, sgGFP2, sgGFP3
ExpressionMammalianPromoterH1, U6, 7SKAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL4 sgRNA(MS2)-MS2-hA3a
Plasmid#112130Purposeempty sgRNA cloning vector with MS2-humanA3aDepositorAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HER2-DMVF-181-N
Plasmid#87622PurposeN-terminal Dronpa fragment fused to the C-terminus of full-length HER2; for BiFC.DepositorAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV4-A
Plasmid#74975PurposeshETV4 shRNADepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-GJA1-20K-V5
Plasmid#49859PurposeEncodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tagDepositorInsertGJA1-20k (GJA1 Human)
TagsV5ExpressionMammalianMutationGJA1-20k fragment corresponds to residues 213-382…PromoterCMVAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-mCh (VE5626)
Plasmid#139772PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with mCherry cDNA under p10 to monitor infection.DepositorInsertmCherry fluorescent protein
PromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBA57-TEV-His12
Plasmid#137033Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66270.1 (BB_A57 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length BBA57, contains signal pep…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG IL13-CAR
Plasmid#157742Purposeknock IL13-CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertChimeric antigen receptor targeting IL13
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(1-976aa)
Plasmid#139689PurposeExpresses N-teminal Flag tagged aa 1-976 of LARS1DepositorAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-EXO1b D173A
Plasmid#68269PurposeBacterial expression of human EXO1 D173A (catalytically-dead) mutantDepositorInsertEXO1 (EXO1 Human)
TagsMxe intein/chitin binding domainExpressionBacterialMutationcatalytically-dead D173A. Please see depositor co…PromoterT7Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pS6-hα2M-H6
Plasmid#128495PurposeExpresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.DepositorAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-KLF7-T2A-PuroR
Plasmid#162346PurposeLentiviral expression of KLF7 under the control of the TetON promoterDepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-pETM30
Plasmid#85768Purposebacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
TagsGST and HisExpressionBacterialMutationcatalytic domain (aa 212-514)PromoterT7-lacAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-BASIC-ARHGAP29 PROMOTER
Plasmid#104155Purposeluciferase reporter with ARHGAP29 promoterDepositorInsertARHGAP29 promoter (ARHGAP29 Human)
UseLuciferaseTagsluciferaseExpressionMammalianPromoterARHGAP29Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W
Plasmid#105036PurposeLentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-STING-C88S-FLAG
Plasmid#198187PurposeMammalian expression of FLAG-tagged mouse STING1 C88SDepositorAvailable SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-SNAP-PD-1
Plasmid#223572PurposeLentiviral expression of human PD-1 with SNAP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-NEUROG3-T2A-PuroR
Plasmid#162341PurposeLentiviral expression of NEUROG3 under the control of the TetON promoterDepositorAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgCIC-1
Plasmid#74966PurposesgCIC-1, puromycin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only