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179,521 results

Showing: 6481 - 6520 of 179521 results

  11. pRS410

    Plasmid
    #11258
    Depositor
    Fred Cross
    Type
    Empty backbone
    Expression
    Yeast
    Available since
    Feb. 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11258-plasmid-map-sequence-id-6932

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  15. LentiGuide_DKO

    Plasmid
    #183193
    Purpose
    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Publication
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183193-plasmid-map-sequence-id-360099

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  16. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  18. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Publication
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172113-plasmid-map-sequence-id-359519

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  25. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

    Plasmid
    #155305
    Purpose
    Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.
    Depositor
    Stanley Qi
    Publication
    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do
    Insert
    mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
    Use
    Lentiviral
    Tags
    2xNLS-3xFLAG
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155305-plasmid-map-sequence-id-306279

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  32. pcDNA3-RAB-EKARev

    Plasmid
    #118474
    Purpose
    ddRFP-based single-color biosensor for monitoring ERK activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-EKARev
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    118474-plasmid-map-sequence-id-243124

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  33. CLYBL-TO-hNIL-BSD-mApple

    Plasmid
    #124230
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    Expression
    Mammalian
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124230-plasmid-map-sequence-id-240604

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  38. pKC-4.04

    Plasmid
    #112085
    Purpose
    Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin
    Depositor
    James Inglese
    Publication
    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3XFLAG firefly luciferase-beta-globin (HBB Human, Photinus pyralis, Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112085-plasmid-map-sequence-id-214511

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  39. pEGFP-N1-IL6

    Plasmid
    #111933
    Purpose
    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells
    Depositor
    Geert van den Bogaart
    Publication
    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Interleukin 6 (IL6 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    CMV
    Available since
    July 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111933-plasmid-map-sequence-id-213785

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Showing: 6481 - 6520 of 179521 results