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We narrowed to 18,759 results for: nls

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Showing: 6561 - 6600 of 18759 results

  1. pN7CAST

    Plasmid
    #190661
    Purpose
    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190661-plasmid-map-sequence-id-385667

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  4. pFB2F

    Plasmid
    #195289
    Purpose
    Bicistronic HSV-TK and mScarlet resistance for positive/negative selection
    Depositor
    Scott Lowe
    Publication
    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HSV-TK 2A mScarlet
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    195289-plasmid-map-sequence-id-382900

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  7. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187957
    Purpose
    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187957-plasmid-map-sequence-id-373706

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  11. pGES201

    Plasmid
    #190197
    Purpose
    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection
    Depositor
    Yuefeng Guan
    Publication
    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190197-plasmid-map-sequence-id-371938

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  14. LentiCRISPRv2 RFP670

    Plasmid
    #187646
    Purpose
    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site
    Depositor
    Martin Carroll
    Insert
    RFP 670
    Use
    Lentiviral
    Tags
    RFP670 and sgRNA cloning site
    Promoter
    EFS (P2A)
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187646-plasmid-map-sequence-id-367454

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  17. mNeonGreen-Smad3

    Plasmid
    #172098
    Purpose
    Expresses fluorescently tagged human Smad3 in mammalian cells.
    Depositor
    Lea Goentoro
    Publication
    Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Smad 3 (Smad3 Mouse)
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    C49S mutation in mCerulean
    Promoter
    CMV
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172098-plasmid-map-sequence-id-341509

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  19. PEA1-Nuclease-Puro

    Plasmid
    #171992
    Purpose
    Delivers all prime editing nuclease components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    171992-plasmid-map-sequence-id-338139

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  22. pCAG-spCas9-N57

    Plasmid
    #169920
    Purpose
    Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.
    Depositor
    Zhili Rong
    Publication
    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-N57
    Use
    CRISPR
    Mutation
    N57 is the PAI domain of SB100X
    Promoter
    CAG
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169920-plasmid-map-sequence-id-336052

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  23. pCAG-spCas9-SB

    Plasmid
    #169919
    Purpose
    Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.
    Depositor
    Zhili Rong
    Publication
    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SB100X
    Use
    CRISPR
    Promoter
    CAG
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169919-plasmid-map-sequence-id-336025

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  28. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Plasmid
    #154430
    Purpose
    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    Depositor
    David Liu
    Publication
    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:
    Insert
    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    VRQR point mutations in SpCas9
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154430-plasmid-map-sequence-id-304586

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  35. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid
    #159281
    Purpose
    A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AsCpf1-HA-2A-GFP
    Use
    Multicistronic vector with both caggs promoter-dr…
    Tags
    3X HA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159281-plasmid-map-sequence-id-311914

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  38. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    153011-plasmid-map-sequence-id-298673

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Showing: 6561 - 6600 of 18759 results