We narrowed to 12,267 results for: shRNA

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pX330_PHF19_sgRNA

Plasmid

#246404

Purpose

Cas9/sgRNA expression plasmid targeting PHF19

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDY156: SUZ12 px330 sgRNA Cas9 plasmid

Plasmid

#170792

Purpose

This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD3-ebony

Plasmid

#83380

Purpose

Drosophila gRNA expression plasmid targets ebony

Depositor

Richard Padgett

Article

Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

Insert

ebony (e Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR/pSUPER+ (575-1)

Plasmid

#17338

Purpose

Gateway entry vector for shRNA expression under H1 promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi; Entry vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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sgPPP1R2

Plasmid

#227391

Purpose

Adhesion module, guide RNA recognizes PPP1R2

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

guide RNA targeting PPP1R2

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMM135

Plasmid

#127216

Purpose

BeYDV replicon with constitutive Luc expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, WUS2, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB6633

Plasmid

#106162

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJR152

Plasmid

#196280

Purpose

Cassette used in dual sgRNA library generation

Depositor

Rebecca Voorhees

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Insert

hU6-sgRNA-CR3

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Level 1 P4 TaU6 guide acceptor

Plasmid

#165600

Purpose

GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

TaU6 promoter::LacZ::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Sgrna acceptor with…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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Level 1 P3 TaU6 guide acceptor

Plasmid

#165599

Purpose

GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

TaU6 promoter::LacZ::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Sgrna acceptor with…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSpyC-GFP

Plasmid

#220191

Purpose

sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMM131

Plasmid

#127214

Purpose

BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, AtSTM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV/MASC_(GLuc)_TOP1

Plasmid

#68439

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TIA1

Plasmid

#106097

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA TIA1

Use

CRISPR

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-RNF103-CHMP3

Plasmid

#140584

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA RNF103-CHMP3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMM136

Plasmid

#127217

Purpose

BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, MP-delta, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pC0051-W85X REPAIR targeting guide cloned into pC0043

Plasmid

#103867

Purpose

Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

W85X targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - CTRL sgRNA

Plasmid

#70662

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pU6_(Gluc)_INT(GenPurpClon)

Plasmid

#68433

Purpose

General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct

Use

CRISPR; Cloning vector for generating int-like co…

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pEMPTY::sgRNA2

Plasmid

#165459

Purpose

Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

Depositor

Inigo Lasa

Article

Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

Insert

sgRNA2

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

SP01

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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