4,200 results

1. pCMV-MMLVgag-3xNES-Cas9

   Plasmid

   #181752

   Purpose

   MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLV-Gag-3xNES-Cas9 (Spy)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-sgRNA hygro

   Plasmid

   #104991

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-KRAS-G12D

   Plasmid

   #116423

   Purpose

   Lentiviral expression of KRAS G12D

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Mutation

   G12D

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti PGK Blast DEST (w524-1)

   Plasmid

   #19065

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-pHluorin_M153R-CD63

   Plasmid

   #172117

   Purpose

   Fluorescent reporter for CD63-positive exosome secretion

   Depositor

   Alissa Weaver

   Article

   Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

   Insert

   CD63 (CD63 Human)
   

   Use

   Lentiviral

   Tags

   pHluorin_M153R in a small extracelluar loop of CD…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSFFV_sfCherry3C(1-10)

   Plasmid

   #117482

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3C(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3C(1-10)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   5 substitutions (K45R, G52D, T106A, K182R, N194D)…

   Promoter

   SFFV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mfn2-YFP

   Plasmid

   #28010

   Depositor

   Richard Youle

   Article

   Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

   Insert

   mitofusin 2 (MFN2 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   silent S508S

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. ER-GCaMP6-210

   Plasmid

   #86919

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-210
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. DHB-mVenus

   Plasmid

   #136461

   Purpose

   Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

   Depositor

   Tobias Meyer, Sabrina Spencer

   Article

   Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

   Insert

   DNA Helicase B (HELB Human)
   

   Use

   Lentiviral

   Tags

   mVenus

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mNeonGreen-Giantin

    Plasmid

    #98880

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Giantin
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJL-TRBO-G

    Plasmid

    #80083

    Purpose

    An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.

    Depositor

    John Lindbo

    Article

    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

    Insert

    GFP
    

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV CEPIA3mt

    Plasmid

    #58219

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA3mt
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmVenus(L68V)-mTurquoise2

    Plasmid

    #60493

    Purpose

    Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    SYFP2-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-pHluorin_M153R-CD63-mScarlet

    Plasmid

    #172118

    Purpose

    Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

    Depositor

    Alissa Weaver

    Article

    Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

    Insert

    CD63 (CD63 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet and pHluorin_M153R in a small extracellu…

    Expression

    Mammalian

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Cre-IRES2-GFP

    Plasmid

    #26646

    Purpose

    Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

    Depositor

    Anjen Chenn

    Article

    Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    GFP and IRES2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. MI-Luciferase-IRES-mCherry

    Plasmid

    #75020

    Purpose

    Encodes the expression of Luciferase and mCherry

    Depositor

    Xiaoping Sun

    Article

    Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

    Inserts

    firefly Luciferase
    mCherry
    

    Use

    Luciferase and Retroviral

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 myc CIITA (P#808)

    Plasmid

    #14650

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Immunity. 2000 Jan . 12(1):61-70.

    Insert

    CIITA (CIITA Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ230 (LbCpf1)

    Plasmid

    #86210

    Purpose

    LbCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    5' and 3' NLS

    Expression

    Plant

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-MMLVgag-3xNES-ABE8e-NG

    Plasmid

    #181754

    Purpose

    MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

    Depositor

    David Liu

    Article

    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

    Insert

    MMLVgag-3xNES-ABE8e-NG
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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