4,142 results

1. pDx_mScarlet3

   Plasmid

   #189754

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-CMV-mCherry-T2A-Puro

   Plasmid

   #72264

   Purpose

   Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

   Depositor

   Kazuhiro Oka

   Insert

   mCherry-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Antares2 c-myc

   Plasmid

   #100027

   Purpose

   A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   Antares2
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1 Lifeact-EGFP

   Plasmid

   #58470

   Purpose

   Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   Lifeact
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer20-Blast

   Plasmid

   #109334

   Purpose

   pInducer20 with added blasticidin resistance

   Depositor

   Jean Cook

   Article

   Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

   Type

   Empty backbone

   Use

   Lentiviral; Inducible expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCBC-DT1T2

   Plasmid

   #50590

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T1, U626t plus, U629p, T2
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWPXL

   Plasmid

   #12257

   Purpose

   2nd generation lentiviral transfer plasmid

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSNAP-tag (m) Vector

   Plasmid

   #101135

   Purpose

   Expression of protein of interest with SNAP-Tag in Mammalian cells

   Depositor

   Ana Egana, New England Biolabs

   Type

   Empty backbone

   Use

   Tags

   SNAP-tag (SNAP26m)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. lentiMPH v2

   Plasmid

   #89308

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Insert

   MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBOB-EF1-FastFUCCI-Puro

    Plasmid

    #86849

    Purpose

    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

    Depositor

    Duncan Jodrell, Kevin Brindle

    Article

    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

    Inserts

    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 and PGK

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-Mito4x-GCaMP6f

    Plasmid

    #127870

    Purpose

    Fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    4 repeats of COX8 signal peptide plus GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSETB/mStayGold

    Plasmid

    #212017

    Purpose

    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-4T1 SHP2 WT

    Plasmid

    #8322

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-NLS-HA-Bxb1

    Plasmid

    #51271

    Purpose

    Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR_EGFPligand

    Plasmid

    #79129

    Purpose

    Lentiviral vector for surface expressed EGFP (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    EGFP surface ligand
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. SP-dCas9-VPR

    Plasmid

    #63798

    Purpose

    SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    SP-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PE6d

    Plasmid

    #207854

    Purpose

    Mammalian expression of PE6d prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6d
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-G3BP1-WT

    Plasmid

    #135997

    Purpose

    WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-CMV-gagpol

    Plasmid

    #35614

    Depositor

    Patrick Salmon

    Insert

    MLV gag and pol genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-CMV-MCS-GFP-SV-puro

    Plasmid

    #73582

    Purpose

    Third generation lentiviral vector expressing GFP and puromycin

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP tag

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge