We narrowed to 14,580 results for: pET

1. pET28 Set9 wt

   Plasmid

   #24082

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-SrtAdelta59

   Plasmid

   #51138

   Purpose

   expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

   Depositor

   Hidde Ploegh

   Article

   Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

   Insert

   S.aureus SrtA delta 59
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pET28a-SpyCatcher

   Plasmid

   #223633

   Purpose

   Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a_T7-ARG2

   Plasmid

   #106474

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. nifF in pET21b

   Plasmid

   #124520

   Purpose

   Azotobacter vinelandii flavodoxin II of Nitrogenase

   Depositor

   Douglas Rees

   Article

   Segal et al Protein Sci. 2017 Oct;26(10):1984-1993.

   Insert

   Flavodoxin II (nifF Azotobacter vinelandii)
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / lac

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET24c(+)-EFTu

   Plasmid

   #31172

   Depositor

   Linda Spremulli

   Article

   Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

   Insert

   E. coli elongation factor Tu (tufB E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pET-IG-mSA

   Plasmid

   #80706

   Purpose

   Expresses monomeric streptavidin (mSA) in bacterial cells

   Depositor

   Matthieu Sainlos

   Article

   Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10

   Insert

   Monomeric streptavidin
   

   Use

   Tags

   10xHis tag and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-PHGDH

   Plasmid

   #74113

   Purpose

   Expression of N-terminal His-tagged

   Depositor

   Lewis Cantley

   Article

   Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Tags

   His-tagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-AncMEcoGII250

   Plasmid

   #205025

   Purpose

   Expressing engineered M.EcoGII with higher thermostability

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 250
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET24a MDH_AQUAR

    Plasmid

    #204239

    Purpose

    Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_AQUAR
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21a.Tat(48-57)-EGFP

    Plasmid

    #165040

    Purpose

    Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    EGFP
    

    Use

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-TRBP

    Plasmid

    #50351

    Purpose

    bacterial expression of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET3a-Ub-K63R

    Plasmid

    #18898

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005 . 301():47-55.

    Insert

    Ubiquitin-K63R (UBC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lysine 63 is replaced with a Argnine residue

    Promoter

    Available since

    Sept. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET24a MDH_9CREN

    Plasmid

    #204243

    Purpose

    Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_9CREN
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETSUMO-TtAgo

    Plasmid

    #210191

    Purpose

    Bacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus

    Depositor

    Phillip Zamore

    Article

    Smith et al Nat Commun. 2019 Jan 17;10(1):272. doi:

    Insert

    TtAgo (TT_P0026 Thermus thermophilus)
    

    Use

    Tags

    6x His tag and SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET11d-RAN

    Plasmid

    #108919

    Purpose

    Expresses human RAN

    Depositor

    Larry Gerace

    Article

    Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

    Insert

    RAN (RAN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a MDHC_YEAST

    Plasmid

    #204232

    Purpose

    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHC_YEAST (MDH2 Budding Yeast)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a(+)- PC-MjMAT

    Plasmid

    #179372

    Purpose

    PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

    Depositor

    Andrea Rentmeister

    Article

    Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

    Insert

    PC-MjMAT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L147A, I351A

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET14b-dabAB2

    Plasmid

    #133013

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Use

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    t7

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET30-2-PSAT1

    Plasmid

    #83908

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    phosphoserine aminotransferase 1 (PSAT1 Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET24c(+)-EFTs

    Plasmid

    #31173

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Ts
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pET21b-P35

    Plasmid

    #11808

    Depositor

    Guy Salvesen

    Article

    Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-8

    Insert

    P35
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

23. +36GFP-LPETG

    Plasmid

    #71754

    Purpose

    +36GFP-LPETG for expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    (GGS)9_(pos36GFP)_6XHis_LPETG
    

    Use

    Tags

    6XHis and Sortase (LPETG)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28-2H12

    Plasmid

    #221186

    Purpose

    His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-30 Ubwt (2)

    Plasmid

    #26685

    Depositor

    Linda Hicke

    Article

    French et al J Biol Chem. 2009 May 1. 284(18):12071-9

    Insert

    Ubiquitin
    

    Use

    Tags

    His and S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pET-X(5010)B1

    Plasmid

    #168019

    Purpose

    Expresses X(5010)B1-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    X(5010)B1-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28_Caspase-4_K356D

    Plasmid

    #214312

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    K356D

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-CV-SNAP25

    Plasmid

    #168031

    Purpose

    Expresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-SNAP25(A141-G206)-mVenus
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET3a-Yuh1

    Plasmid

    #18895

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005 . 301():47-55.

    Insert

    Ubiquitin C-terminal hydrolase I (YUH1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pET28a-MSP1D1delltaH4

    Plasmid

    #71713

    Purpose

    Membrane scaffold protein (MSP), helix 4 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28-hTDG

    Plasmid

    #70758

    Purpose

    bacterial expression of human TDG

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    TDG (TDG Human)
    

    Use

    Tags

    6-HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lac operon

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET11d-hUba2

    Plasmid

    #53136

    Purpose

    E.coli expression of full length untagged human Uba2 (SAE2)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    UBA2 (UBA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-Galectin-8 FL

    Plasmid

    #68805

    Purpose

    Express Galectin-8 FL protein in Bacterial cells

    Depositor

    Hyun Kyu Song

    Article

    Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

    Insert

    Galectin-8 (LGALS8 Human)
    

    Use

    Tags

    N-terminal 6-histidine-tag

    Expression

    Bacterial

    Mutation

    No mutation

    Promoter

    T7

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a_T5-ARG1

    Plasmid

    #106476

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a-PFO

    Plasmid

    #92109

    Purpose

    Recombinant expression in E.Coli

    Depositor

    Feng Shao

    Article

    Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

    Insert

    PFO
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Delta27aa

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET-X(4130)B1

    Plasmid

    #168018

    Purpose

    Expresses X(4130)B1-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    X(4130)B1-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-SpySrtA

    Plasmid

    #51139

    Purpose

    expression plasmid for N-terminal his tagged S.pyogenes SrtA

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.pyogenes SrtA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-80

    Promoter

    T7

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET51b(+)_HaloTag9

    Plasmid

    #169324

    Purpose

    Expression of HaloTag9 in bacterial cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET11a-Z-NGFP

    Plasmid

    #52734

    Purpose

    IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    NGFP+Z-fusion
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge