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We narrowed to 1,273 results for: grna cloning vector

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Showing: 801 - 850 of 1273 results

  1. pSIREN-RetroQ-BPTF-sh27

    Plasmid
    #73668
    Purpose
    pSIREN-RetroQ vector containing shRNA sequence to BPTF
    Depositor
    Joe Landry
    Insert
    shRNA #27 to BPTF (BPTF, Bptf Mouse, Human)
    Use
    Retroviral
    Available Since
    April 13, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    73668-plasmid-map-sequence-id-141990

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  2. pGMF1-M

    Plasmid
    #242203
    Purpose
    OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in monocots.
    Depositor
    Kate Creasey Krainer
    Article
    Abate et al bioRxiv 2025.11.09.687425
    Insert
    Level1 OsU6-2p::sgRNA1 for subcloning of 20 bp target sequence
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    OsU6-2p
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242203-plasmid-map-sequence-id-490170

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  3. pGMF2-M

    Plasmid
    #242204
    Purpose
    OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in monocots.
    Depositor
    Kate Creasey Krainer
    Article
    Abate et al bioRxiv 2025.11.09.687425
    Insert
    Level1 OsU6-2p::sgRNA2 for subcloning of 20 bp target sequence
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    OsU6-2p
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242204-plasmid-map-sequence-id-490169

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  4. pGMF1-D

    Plasmid
    #242205
    Purpose
    AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in dicots.
    Depositor
    Kate Creasey Krainer
    Article
    Abate et al bioRxiv 2025.11.09.687425
    Insert
    Level1 AtU6-26p::sgRNA1 for subcloning of 20 bp target sequence
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    AtU6-26p
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242205-plasmid-map-sequence-id-490168

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  5. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166698-plasmid-map-sequence-id-329407

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  6. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166700-plasmid-map-sequence-id-329224

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  7. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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  8. pX458-tRNA-SpCas9

    Plasmid
    #195183
    Purpose
    Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.
    Depositor
    Stephanie Roessler
    Insert
    Cas9
    Use
    CRISPR
    Promoter
    tRNA-Gln
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    195183-plasmid-map-sequence-id-423143

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  9. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available Since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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  20. MultiMate-PE3 HEK3

    Plasmid
    #206273
    Purpose
    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    Use
    CRISPR; Recombinant baculovirus production
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206273-plasmid-map-sequence-id-406645

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  21. MultiMate-PE2 HEK3

    Plasmid
    #206274
    Purpose
    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    Use
    CRISPR; Recombinant baculovirus production, cre-a…
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206274-plasmid-map-sequence-id-406650

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  26. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein i…
    Available Since
    Oct. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68709-plasmid-map-sequence-id-125636

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  31. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    U1a promoter
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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  34. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Article
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available Since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    60820-plasmid-map-sequence-id-371636

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  38. MCS_BsmBI

    Plasmid
    #241170
    Purpose
    Middle entry vector for mini-Golden subcloning platform; MCS; BsmBI
    Depositor
    Junsu Kang
    Insert
    MCS; BsmBI
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241170-plasmid-map-sequence-id-482039

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  39. memb-mNeongreen

    Plasmid
    #241175
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241175-plasmid-map-sequence-id-482019

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  41. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

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  45. SA-mCherry-pA

    Plasmid
    #241212
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mCherry
    Depositor
    Junsu Kang
    Insert
    mCherry
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241212-plasmid-map-sequence-id-484620

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  46. SA-EGFP-pA

    Plasmid
    #241211
    Purpose
    Middle entry vector for mini-Golden subcloning platform; eGFP
    Depositor
    Junsu Kang
    Insert
    eGFP
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241211-plasmid-map-sequence-id-482022

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  47. SA-TCGC-mNeongreen

    Plasmid
    #241203
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241203-plasmid-map-sequence-id-484622

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  48. SA-memb-mNeongreen

    Plasmid
    #241207
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241207-plasmid-map-sequence-id-482175

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  49. pMid-TCGC-mNeongreen

    Plasmid
    #241171
    Purpose
    Middle entry vector for mini-Golden subcloning platform; mNeongreen
    Depositor
    Junsu Kang
    Insert
    mNeongreen
    Use
    Mini-golden
    Mutation
    TCGC linker
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241171-plasmid-map-sequence-id-482081

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Showing: 801 - 850 of 1273 results