We narrowed to 10,975 results for: NSI;

1. Kif1A(1-489)-DHFR-myc

   Plasmid

   #117833

   Purpose

   Constitutively active Kinesin-3 motor tagged with DHFR and myc

   Depositor

   Thomas Schwarz

   Article

   Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

   Insert

   Kif1A (1-489) (Kif1a Mouse)
   

   Use

   Tags

   DHFR and myc

   Expression

   Mutation

   only aa 1-489 (motor domain)

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. gRNA-hIRF-1 #12/pSIR

   Plasmid

   #61080

   Purpose

   Expresses a guide RNA (gRNA) to target human IRF-1 promoter

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   gRNA_hIRF1 promoter #12 (IRF1 Human)
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHH0103_CRK-1/2

   Plasmid

   #91367

   Purpose

   Protein expression and purification of human SH3 domain construct CRK-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   CRK-1/2 (CRK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330A-Yap1-E1-#1

   Plasmid

   #171517

   Purpose

   deletion of a genomic locus in Yap1 gene

   Depositor

   Hans Schöler

   Article

   Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330A-Yap1-E2-#1

   Plasmid

   #171518

   Purpose

   deletion of a genomic locus in Yap1 gene

   Depositor

   Hans Schöler

   Article

   Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL3-Tet1 promoter A

   Plasmid

   #63881

   Purpose

   Luciferase reporter plasmid used to study transcriptional control of murine Tet1

   Depositor

   Kian Peng Koh

   Article

   Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

   Insert

   Tet1 promoter fragment A
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAS_4xMx PylT C41CA_FLAG_Mx PylRS

   Plasmid

   #140013

   Purpose

   Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. F-MED28

   Plasmid

   #15368

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

   Insert

   MED28 (MED28 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. mEos3.2trLAT-2P

   Plasmid

   #203748

   Purpose

   Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trLAToC27AC30A (Lat Mouse)
   

   Use

   Tags

   ER translocation signal - mEos3.2

   Expression

   Mammalian

   Mutation

   C27A, C30A

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHH0103_ARHGAP4-1/1

    Plasmid

    #91518

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP4-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP4-1/1 (ARHGAP4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHH0103_ARHGAP9-1/1

    Plasmid

    #91442

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP9-1/1 (ARHGAP9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-Tet1 promoter B

    Plasmid

    #63882

    Purpose

    Luciferase reporter plasmid used to study transcriptional control of murine Tet1

    Depositor

    Kian Peng Koh

    Article

    Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

    Insert

    Tet1 promoter fragment B
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Sumo3 PolQM1 deltains1

    Plasmid

    #78461

    Purpose

    Inducible expression in E. coli

    Depositor

    Susan Wallace, Sylvie Doublie

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2149-2170; Q2513R (please see…

    Promoter

    T7

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

    Plasmid

    #140014

    Purpose

    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y126A, Y197F

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX304_zeo_eGFP_V5

    Plasmid

    #160096

    Purpose

    Expresses eGFP in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-mCherry-SPASTIN, F124D

    Plasmid

    #180648

    Purpose

    Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, F124D mutation, has siRNA resistan…

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVL47 - pSCR>>

    Plasmid

    #116013

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p260 pCMV-CRE-M-AR(wt)

    Plasmid

    #12494

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Insert

    CRE-M AR (AR Human)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    CRE-M and AR ligand binding domain

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

19. MAC_C_H31

    Plasmid

    #111666

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    H31 (H3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRetroX-TRE3G_NT-GSDMD_I105N_

    Plasmid

    #201174

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    aa1-276 only, I105N

    Promoter

    TRE3G

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a_6HT-TBP6.7(R49A)

    Plasmid

    #112726

    Purpose

    Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R49A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R49A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)

    Plasmid

    #39858

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    C11orf51 (ANAPC15 Human)
    

    Use

    Tags

    IRES2-mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

23. TRS6E1b-luc(-732/-721) mutant 5

    Plasmid

    #45388

    Depositor

    Thomas Gelehrter

    Article

    Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

    Insert

    6 copies of hPAI-1 promoter -732/-721 mutated at -725/723
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    -725/-723 mutated from gtt to ccc

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

24. Emerald-MannII-N-10

    Plasmid

    #54150

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    MannII (Man2a1 Mouse)
    

    Use

    Tags

    Emerald

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII aa 1-112 of MannII

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

25. pBW_0001

    Plasmid

    #102833

    Purpose

    Binary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 Schomburgk (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRD408

    Plasmid

    #178199

    Purpose

    Expression of H-NS from its natural promoter

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    phns + hns (hns Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    hns promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNCST-AausFP3

    Plasmid

    #129501

    Purpose

    Expresses AausFP3 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausFP3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_FUT8-1/1

    Plasmid

    #91270

    Purpose

    Protein expression and purification of human SH3 domain construct FUT8-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FUT8-1/1 (FUT8 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTRE Tight ArchT-GFP

    Plasmid

    #79627

    Purpose

    Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

    Depositor

    Yasunori Hayashi

    Article

    Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

    Insert

    ArchT
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ArchT is a more sensitive version of Arch (archae…

    Promoter

    Tet responsive Ptight promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pVL46 - pWOX4>>

    Plasmid

    #116012

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDEST40-2XHA-Src-KD

    Plasmid

    #140312

    Purpose

    Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Contains only the KD region

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-FLAG-MTOR-D2512H

    Plasmid

    #69017

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-D2512H (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Aspartate 2512 to Histidine

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pMSCV-GSDMD_NoCys-Myc

    Plasmid

    #201169

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    C39A, C57A, C77A, C122A, C265A, C192A

    Promoter

    MESV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MAC_C_HSP7C

    Plasmid

    #111667

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    HSP7C (HSPA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR

    Plasmid

    #75136

    Purpose

    Retroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1116
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR

    Plasmid

    #75135

    Purpose

    Retroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1061
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGL-p21UTRm1

    Plasmid

    #20878

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 1 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 1 (Position ~420) GC…

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

38. pHH0103_GAS7-1/1

    Plasmid

    #91301

    Purpose

    Protein expression and purification of human SH3 domain construct GAS7-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GAS7-1/1 (GAS7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNCST-AausFP4

    Plasmid

    #129502

    Purpose

    Expresses AausFP4 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausFP4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TTR C10S S100C

    Plasmid

    #190010

    Purpose

    Expression of human TTR in E coli

    Depositor

    Peter Wright

    Article

    Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, S100C

    Promoter

    t7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge