We narrowed to 21,604 results for: ESC

1. pKL003

   Plasmid

   #98919

   Purpose

   Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

   Insert

   PROPS
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23118 - Strong Constitutive

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDH-puro-CMV-CC3AI

   Plasmid

   #78909

   Purpose

   Indicator of caspase-3-like protease activity

   Depositor

   Binghui Li

   Article

   Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

   Insert

   CC3AI
   

   Use

   Lentiviral

   Tags

   HA and Myc

   Expression

   Mutation

   S320A, deletion of AA1-200

   Promoter

   CMV

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

3. CAG FUCCI

   Plasmid

   #175266

   Purpose

   Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

   Depositor

   James Monaghan

   Article

   Duerr et al Development. 2022 Mar 15;149(6). pii: 27

   Insert

   FUCCI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Str-KDEL_SBP-EGFP-Ecadherin

   Plasmid

   #65286

   Purpose

   synchronize trafficking of Ecadherin from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Use

   Tags

   EGFP and IL-2 Signal Sequence and Streptavidin Bi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-mTurquoise2-CNA35

   Plasmid

   #61602

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mTurqouise and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-N1 alpha-actinin 1

   Plasmid

   #11908

   Depositor

   Carol Otey

   Article

   Edlund et al Cell Motil Cytoskeleton. 2001 Mar . 48(3

   Insert

   alpha-actinin 1 (ACTN1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mEos4b-N1

   Plasmid

   #54814

   Purpose

   Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Loren Looger, Michael Davidson

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Type

   Empty backbone

   Use

   Tags

   mEos4b

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pBDC

   Plasmid

   #135188

   Purpose

   Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   CmR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   cat

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-EF1a-mCherry-MinD-IRES-Puro

   Plasmid

   #213112

   Purpose

   Expression of mCherry-MinD in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21d-myc-BirA

    Plasmid

    #109424

    Purpose

    Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    BirA (birA )
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPEG

    Plasmid

    #162466

    Purpose

    Entry vector for gRNA insertion in plant prime editing expression vector

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Type

    Empty backbone

    Use

    Entry vector for grna

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmScarlet3-Peroxi_C1

    Plasmid

    #189769

    Purpose

    Construct for labelling peroxisomes in mammalian cells with peroxisomal targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Peroxi
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZR060_CROPseq-sgCtrl-CRISPRi

    Plasmid

    #180269

    Purpose

    Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mNeonGreen-DEVD-NanoLuc

    Plasmid

    #98287

    Purpose

    caspase-3 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTD-NStrepHis

    Plasmid

    #45936

    Purpose

    use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-miRFP680-P2A-GFP

    Plasmid

    #197164

    Purpose

    Expresses the protein of miRFP680-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP680-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-Trx-mSA2

    Plasmid

    #52320

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, S tag, TEV site, and Thioredoxin

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV.hSyn-FLEX.iGABASnFR

    Plasmid

    #112163

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Has Service

    AAV1

    Insert

    iGABASnFR
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDAS12395_PEAR-GFP_2.0-preedited

    Plasmid

    #177185

    Purpose

    PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLPC-Flag-PML-IV

    Plasmid

    #62804

    Purpose

    expresses the isoform IV of PML (promyelocytic leukemia protein)

    Depositor

    Gerardo Ferbeyre

    Article

    Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

    Insert

    PML-IV (PML Human)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pmTurquoise2-Peroxi

    Plasmid

    #36203

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    Peroxisomal targeting sequence
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EcNusGFL

    Plasmid

    #113122

    Purpose

    Expresses full-length E. coli NusG

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    NusG (nusG Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEB2-mKate2

    Plasmid

    #104009

    Purpose

    Plasmid encoding mKate2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mKate2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, V45A, …

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZR069_CROPseq-sgPTPRC-CRISPRi

    Plasmid

    #180271

    Purpose

    Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    PTPRC (CD45) sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-itis pSel

    Plasmid

    #195344

    Purpose

    Selection plasmid expressing non-fluorescent EGFP A111V

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A111V

    Promoter

    J23119

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-mAmetrine-CNA35

    Plasmid

    #61604

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mAmetrine and CNA35
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-LSSmOrange-CNA35

    Plasmid

    #61605

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    LSSmOrange and CNA35
    

    Use

    Tags

    His tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RA-NES

    Plasmid

    #61019

    Purpose

    ddRFP A-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A
    

    Use

    Tags

    HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-PKCA WT

    Plasmid

    #195202

    Purpose

    Mammalian expression vector for GFP tagged PKCA.

    Depositor

    Peter Parker, David Bryant

    Article

    Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

    Insert

    PRKCA (PRKCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mEos4b-C1

    Plasmid

    #54812

    Purpose

    Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mEos4b

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pAAV-hSYN-miRFP670nano3-WPRE-hGHpA

    Plasmid

    #184688

    Purpose

    Mammalian expression, miRFP670nano3

    Depositor

    Axel Nimmerjahn, Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSYN

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SupD v2

    Plasmid

    #198598

    Purpose

    Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SupD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFlp-Tc

    Plasmid

    #222336

    Purpose

    flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB )
    flp (flp )
    cI (cI )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cl, flp, and sacB

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP-C1X-Nup93

    Plasmid

    #87336

    Purpose

    To express human Nup93 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP93 (NUP93 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMP2463

    Plasmid

    #107774

    Purpose

    Expression of EGFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EGFP in pBBR1-mcs5
    

    Use

    Tags

    pLacZ-EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    pLacZ

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PIP2-GFP(ST) sensor

    Plasmid

    #170356

    Purpose

    GFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

    Depositor

    Kees Jalink

    Article

    van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

    Insert

    GFP(ST)-PH (PLCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Str-KDEL_SBP-mCherry-Ecadherin

    Plasmid

    #65287

    Purpose

    synchronize trafficking of Ecadherin from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-CAG-miniGFP1-P2A-FusionRed

    Plasmid

    #188732

    Purpose

    Express miniGFP1 and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP1
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FusionRed-pBAD

    Plasmid

    #54677

    Purpose

    Localization: Protein Expression Vector , Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Type

    Empty backbone

    Use

    Tags

    6xHis and FusionRed

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

41. pDR111_mannose_Bgal no NLS

    Plasmid

    #188399

    Purpose

    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    Promoter

    D-mannose

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

    Plasmid

    #191338

    Purpose

    Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11

    Plasmid

    #162037

    Purpose

    Background-free mRNA labeling tripartite superfolder GFP system

    Depositor

    Hye Yoon Park

    Article

    Park et al RNA. 2020 Jan;26(1):101-109. doi: 10.126

    Insert

    GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209729

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHybrid-Electra1

    Plasmid

    #179474

    Purpose

    Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Inserts

    Electra1
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pRSFDUET-1_VNp-anti-6xHis mAb

    Plasmid

    #214952

    Purpose

    Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-6xHis mAb heavy chain
    VNp-anti-6xHis mAb light chain
    

    Use

    Tags

    VNp6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBiFC-bJunVN155(I152L)

    Plasmid

    #27098

    Depositor

    Chang-Deng Hu

    Article

    Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

    Insert

    bJunVN155(I152L) (Jun Rat, and Aequorea Victoria)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    I152L in Venus(1-154)

    Promoter

    Available since

    Jan. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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48. pTH847-gst-optsc

    Plasmid

    #96908

    Purpose

    Plasmid for yeast expression of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    yeast codon optimised glutathione-S transferase
    

    Use

    Tags

    8xhistidine

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAAV-CAG-emiRFP670-P2A-GFP

    Plasmid

    #197160

    Purpose

    Expresses the protein of emiRFP670-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    emiRFP670-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

    Plasmid

    #175547

    Purpose

    Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag7 and Tomm20-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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