Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

178,651 results

Showing: 5161 - 5190 of 178651 results

  2. pcDNA3-ExRai-AMPKAR

    Plasmid
    #192446
    Purpose
    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.
    Depositor
    Jin Zhang
    Publication
    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AMPKAR
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192446-plasmid-map-sequence-id-379139

    Enlarge

  4. pTwist-SARS-CoV-2 Δ18 B.1.1.529

    Plasmid
    #179907
    Purpose
    Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Publication
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212;…
    Promoter
    CAG
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179907-plasmid-map-sequence-id-351282

    Enlarge

  6. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162667-plasmid-map-sequence-id-321443

    Enlarge

  12. YE1-BE4max

    Plasmid
    #138155
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE1)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138155-plasmid-map-sequence-id-272340

    Enlarge

  13. pmiRFP680-N1

    Plasmid
    #136557
    Purpose
    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)
    Depositor
    Vladislav Verkhusha
    Publication
    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miRFP680
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136557-plasmid-map-sequence-id-271516

    Enlarge

  14. hSynapsin-PE2-Cterminal-lenti

    Plasmid
    #135956
    Purpose
    Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Publication
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) C-terminal
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    MMLV RT(D200N, T306K, W313F, T330P, L603W)
    Promoter
    hSynapsin
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135956-plasmid-map-sequence-id-269513

    Enlarge

  20. pC1-SypHer3s-dmito

    Plasmid
    #108119
    Purpose
    Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.
    Depositor
    Vsevolod Belousov
    Publication
    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SypHer3s-dmito
    Tags
    ratiometric YFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108119-plasmid-map-sequence-id-207897

    Enlarge

  23. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid
    #99248
    Purpose
    single-vector system with EGFP reporter for use in CROPseq knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99248-plasmid-map-sequence-id-191832

    Enlarge

  26. pCMV-MbPylRS(DiZPK)

    Plasmid
    #91706
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest
    Depositor
    P. Chen
    Publication
    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5
    Inserts
    pylS
    pylT
    Expression
    Mammalian
    Mutation
    L274A, C313S
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91706-plasmid-map-sequence-id-183279

    Enlarge

Showing: 5161 - 5190 of 178651 results