163,606 results

1. pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227891

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   VPS35 homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227892

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   EEA1 homology arms with mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227900

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227901

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227902

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   PEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227904

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227905

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHEE401_GV_MOD

   Plasmid

   #194874

   Purpose

   mCherry selection in Arabidopsis seeds in T1 and T2

   Depositor

   Wolfgang Busch

   Article

   Ruiz Rosquete et al Plant J. 2025 Jan;121(1):e17177. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   egg cell-specific

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCN38-ktrA-yfp

   Plasmid

   #227636

   Purpose

   c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   ktrA-yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM236 Ubc4

    Plasmid

    #226361

    Purpose

    Expression of yeast Ubc4

    Depositor

    Andreas Martin

    Article

    Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

    Insert

    Ubc4 (UBC4 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIVEX2.3-Theo-3c-EGFP

    Plasmid

    #217526

    Purpose

    Theo-3c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

    Depositor

    Keisuke Fukunaga

    Article

    Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

    Insert

    Theo-3c riboswitch-regulated EGFP
    

    Use

    In vitro transcription-translation

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cut190*_pET21b

    Plasmid

    #215404

    Purpose

    Expression construct for Cut190* gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Cut190*
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S226P/R228S

    Promoter

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEB018

    Plasmid

    #215568

    Purpose

    Fuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locus

    Depositor

    Robin Ohm

    Insert

    3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
    

    Use

    Fungal homologous recombination plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV_FKBP-V5-ALX1

    Plasmid

    #215023

    Purpose

    AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human ALX1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    ALX homeobox 1 (ALX1 Human)
    

    Use

    AAV

    Tags

    FKBP-V5

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-VcRibB

    Plasmid

    #187977

    Purpose

    Expresses 3,4-dihydroxy-2-butanone 4-phosphate synthase RibB from Vibrio cholerae

    Depositor

    Audrey Lamb

    Article

    Kenjic et al J Am Chem Soc. 2022 Jul 20;144(28):12769

    Insert

    VcRibB
    

    Use

    Tags

    Hexahistag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF1046

    Plasmid

    #143748

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BAZ1B (BAZ1B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(-)-Green Falcan1

    Plasmid

    #199705

    Purpose

    green fluorescent indicator for cAMP imaging

    Depositor

    Tetsuya Kitaguchi

    Article

    Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

    Insert

    Green Falcan1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJBL3907

    Plasmid

    #193140

    Purpose

    Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter

    Depositor

    Julius Lucks

    Article

    Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

    Insert

    Clostridium beijerinckii (Cbe) pfl ZTP riboswitch with sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJBL6090

    Plasmid

    #193142

    Purpose

    Clostridium beijerinckii pfl ZTP riboswitch A26∆ OFF control with sfGFP reporter

    Depositor

    Julius Lucks

    Article

    Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

    Insert

    Cbe ZTP Riboswitch sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Deleted A26 to disable ligand-binding

    Promoter

    J23119

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Portal_PhiV1

    Plasmid

    #188811

    Purpose

    Portal protein from phage PhiV1

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Portal
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Terminase_ST32

    Plasmid

    #188850

    Purpose

    Large terminase from phage ST32

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Terminase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EpAvs1

    Plasmid

    #188788

    Purpose

    Avs1 from Erwinia piriflorinigrans CFBP5888

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    EpAvs1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    bla

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SpPLK1-GFP

    Plasmid

    #185573

    Purpose

    to see protein dynamics

    Depositor

    Mamiko Yajima

    Article

    Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

    Insert

    PLK1
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

    Plasmid

    #179003

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EGFP-BAF

    Plasmid

    #101772

    Purpose

    Expresses EGFP tagged BAF in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-MmPeg10rc4

    Plasmid

    #174858

    Purpose

    Expresses recoded mouse Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Mus musculus PEG10 (Peg10 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

    Plasmid

    #188767

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FLAG-Pol-II WT

    Plasmid

    #35175

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC19 - T7 pro - IRES - EGFP

    Plasmid

    #138586

    Purpose

    Express EGFP with an upstream T7 promoter in pUC19 backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge