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Showing: 5161 - 5190 of 157662 results

  3. GPER-DuET

    Plasmid
    #213256
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPER (GPER1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213256-plasmid-map-sequence-id-430806

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  5. pCHAP3_Sap

    Plasmid
    #206849
    Purpose
    A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206849-plasmid-map-sequence-id-419973

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  6. pCHAP2

    Plasmid
    #206847
    Purpose
    A plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206847-plasmid-map-sequence-id-419939

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  7. pCHAP1

    Plasmid
    #206846
    Purpose
    A plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206846-plasmid-map-sequence-id-419938

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  10. TFORF3334

    Plasmid
    #144810
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SP100 (SP100 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144810-plasmid-map-sequence-id-404394

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  12. pPANI2A2E2-Crimson

    Plasmid
    #193758
    Purpose
    Expresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.
    Insert
    E2-Crimson
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PANI2 synthetic promoter carrying binding sites f…
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    193758-plasmid-map-sequence-id-389741

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  14. pD649-HAsp-PDCD1LG2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156612
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    PDCD1LG2 (PDCD1LG2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156612-plasmid-map-sequence-id-302333

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  15. pDUAL_tacI

    Plasmid
    #155170
    Purpose
    BaSP and CuCBP co-expression: tacI promoter controls BaSP expression
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    155170-plasmid-map-sequence-id-311666

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  21. pNeae2

    Plasmid
    #168300
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:
    Insert
    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    Use
    Tags
    6xHis, E-tag, and myc-tag
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168300-plasmid-map-sequence-id-334450

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  28. mCherry-LaminB1-10

    Plasmid
    #55069
    Purpose
    Localization: Nuclear Envelope, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    55069-plasmid-map-sequence-id-204489

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  30. mCherry-LaminA-C-18

    Plasmid
    #55068
    Purpose
    Localization: Nuclear Envelope, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Lamin A (LMNA Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55068-plasmid-map-sequence-id-200503

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Showing: 5161 - 5190 of 157662 results