We narrowed to 13,571 results for: GAN

1. APEX2-GBP

   Plasmid

   #67651

   Purpose

   Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2-GBP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Opto-CRAC version 1

   Plasmid

   #101245

   Purpose

   Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

   Depositor

   Yubin Zhou

   Article

   He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

   Insert

   LOV2 and STIM1 cytosolic fragment
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-EF1adCas9VP64T2ABsd-W

   Plasmid

   #112919

   Purpose

   Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   dCas9VP64-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human EF1a promoter

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUAS-V5-TurboID-NES

   Plasmid

   #116904

   Purpose

   Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

   Depositor

   Norbert Perrimon

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID
   

   Use

   Drosophila expression

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRECT_23A

   Plasmid

   #91138

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-pelB-LaG-16

   Plasmid

   #172746

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-16
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_TRE_Ecad

   Plasmid

   #133805

   Purpose

   inducible expression of Ecad in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   Homo sapiens cadherin 1 (CDH1) (CDH1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CSII-IDR2-chimpTRIM5-FOS

   Plasmid

   #79065

   Purpose

   Express chimpanzee TRIM5alpha in mammalan cells

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   TRIM5alpha(chimpanzee)-FOS
   

   Tags

   OneSTrEP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_B2520

   Plasmid

   #91077

   Purpose

   Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   OsU6

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC-ZEAXipi

    Plasmid

    #53287

    Purpose

    Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

    Plasmid

    #136262

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2 8xSTAR-sTomato-NLS. PGK-puro

    Plasmid

    #136261

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEYFP-Sec24C

    Plasmid

    #66608

    Purpose

    YFP fusion of Sec24C

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24C (SEC24C Human)
    

    Expression

    Mammalian

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-eDHFR-MCS

    Plasmid

    #172108

    Purpose

    Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR

    Depositor

    Shinya Tsukiji

    Article

    Ishida et al J Am Chem Soc. 2013 Aug 28;135(34):12684

    Insert

    EGFP-eDHFR-20aa (folA E. coli)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. human STIM1 YFP

    Plasmid

    #19754

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. NID1-bio-His

    Plasmid

    #52068

    Purpose

    Expresses full-length Nidogen-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    NID1 (NID1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pfa6a PhoCl-TsCC(B)::KANMX

    Plasmid

    #185906

    Purpose

    Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    PhoCl-TsCC(B)
    

    Tags

    PhoCl fluorophore with C-terminal TsCC(B)

    Expression

    Yeast

    Promoter

    SP6

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

    Plasmid

    #196192

    Purpose

    human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
    

    Use

    Znf donor

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ePB-PURO-TT-PA-CRE

    Plasmid

    #179584

    Purpose

    ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    PA-MAG-CRE
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. SynTagMA_pre

    Plasmid

    #119738

    Purpose

    Synaptic Tag for Mapping Activity

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

    Insert

    SynTagMA_pre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hsyn1

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pENTR_sfGFP-TurboID

    Plasmid

    #240226

    Purpose

    Gateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    sfGFP-TurboID
    

    Use

    Gateway shuttle vector

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-H2B-Venus

    Plasmid

    #87614

    Purpose

    Venus (YFP) fused to histone H2B.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    H2B-Venus (H2BC8 Human, Synthetic)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSecTag-ATX

    Plasmid

    #17839

    Depositor

    Steven Rosen

    Article

    Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

    Insert

    Autotaxin (ENPP2 Human)
    

    Expression

    Mammalian

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

25. FAB(ER-mito)

    Plasmid

    #214427

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3-LifeAct-FRB-Venus-173-N

    Plasmid

    #87616

    Purpose

    FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-FRB-Venus173-N
    

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pfa6a mScarlet-TsCC(B)::KANMX

    Plasmid

    #176397

    Purpose

    Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-TsCC(B)
    

    Tags

    mScarlet fused to TsCC(B)

    Expression

    Bacterial and Yeast

    Promoter

    none

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET22b_PstS_3

    Plasmid

    #78200

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,I76G,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C,I76G,A197C (please see depositor comment bel…

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.tgoGAP1

    Plasmid

    #78737

    Purpose

    Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

    Depositor

    Teresa Alonso

    Article

    Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Insert

    tgoGAP1
    

    Tags

    N terminal 81 amino acids of human galactosyltran…

    Expression

    Mammalian

    Mutation

    D117A/D119A/D163A in aequorin moiety

    Promoter

    CMV

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pME-MLS-KillerRed

    Plasmid

    #115517

    Purpose

    Middle entry plasmid containing membrane bound KillerRed fluorophore

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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31. MSCV Puro SCRIB WT

    Plasmid

    #88886

    Purpose

    SCRIB WT in MSCV backbone

    Depositor

    Senthil Muthuswamy

    Article

    Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

    Insert

    SCRIB (SCRIB Human)
    

    Use

    Retroviral

    Tags

    N/A

    Mutation

    N/A

    Promoter

    MSCV promoter

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21-pelB-LaM-6

    Plasmid

    #172771

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-6
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET21-pelB-LaTdT-39

    Plasmid

    #228980

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-39
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMM534

    Plasmid

    #112530

    Purpose

    pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Insert

    luxCDABE
    

    Expression

    Bacterial

    Promoter

    PL(HrtO)

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDIRECT_10A

    Plasmid

    #91207

    Purpose

    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pQE60 Q58L wgMDH

    Plasmid

    #204246

    Purpose

    Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Q58L

    Promoter

    T5

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAC-EHER

    Plasmid

    #53262

    Purpose

    Contains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

    Insert

    crtE, idi, crtX, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167942

    Purpose

    Inducible NKX2-1 overexpression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    TTF1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Promoter

    tetON

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES

    Plasmid

    #124707

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NES

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR2 DD (E488Q)_NES
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutant

    Promoter

    CMV

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge