We narrowed to 13,226 results for: 109

1. pBW101ParsR-gfp

   Plasmid

   #78636

   Purpose

   pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

   Insert

   J101-rbs32-arsR-t-ParsR-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   see insert

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pVDM1001

   Plasmid

   #134660

   Purpose

   CRISPR delivery and repair template delivery vector

   Depositor

   Willem van Schaik

   Article

   de Maat et al FEMS Microbiol Lett. 2019 Nov 1;366(22).

   Insert

   CRISPR guide RNA array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. double cut NH-donor

   Plasmid

   #83576

   Purpose

   eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   eGFP
   

   Use

   Nhej donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAID1.2-EF1a-NmScarlet-mAID

   Plasmid

   #140617

   Purpose

   Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGWB602-RBCS-tagRFP

   Plasmid

   #213868

   Purpose

   Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

   Insert

   RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRG705_lexO-AsiSI_LexA-TF_LEU2MX

   Plasmid

   #154817

   Purpose

   Inducible expression of AsiSI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AsiSI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPEY-GAL-Kan

   Plasmid

   #232099

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPEY-GAL-Hyg

   Plasmid

   #232098

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. gh22

   Plasmid

   #106695

   Purpose

   expression of gRNA targeting COSMC

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   COSMC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

10. LifeAct-MTED

    Plasmid

    #217498

    Purpose

    Expresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin

    Depositor

    Erik Dent

    Article

    Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

    Insert

    LifeAct-mScarlet-MTED
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAID1.2-EF1a-CmCherry-mAID

    Plasmid

    #140604

    Purpose

    Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPEY-Z3-Nat

    Plasmid

    #232100

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas9-EcRT-Z3-Hyg

    Plasmid

    #232095

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p-G4PID-HA

    Plasmid

    #209639

    Purpose

    Expression G4PID-HA in mammalian cells

    Depositor

    Xiang Zhou

    Article

    Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

    Insert

    G4PID (DHX36 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-Rabenosyn-5

    Plasmid

    #37538

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    Rabenosyn5 (RBSN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-dfROX

    Plasmid

    #137170

    Purpose

    Lentiviral transfer vector for expression of roGFP2 and HyPer Red

    Depositor

    Shaun McCullough

    Article

    Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

    Insert

    roGFP2-2A-HyPer Red
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. JH02

    Plasmid

    #73511

    Purpose

    mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mCherryo::PH::tbb-2
    

    Use

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    pmex-5

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiAsCpf1-3xMYC-blast

    Plasmid

    #127750

    Purpose

    Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal

    Depositor

    Giulio Draetta

    Article

    Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

    Insert

    AsCpf1
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLC-237

    Plasmid

    #73191

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    ParaB-relE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ParaB

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLC-246

    Plasmid

    #73190

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-tse2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMIEG3-JunB

    Plasmid

    #40349

    Depositor

    Alexander Dent

    Article

    Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

    Insert

    JunB (Jun Mouse)
    

    Use

    Retroviral

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

    Plasmid

    #211840

    Purpose

    Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA and mCherry

    Expression

    Plant

    Mutation

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCI-neo VPS54-13Myc

    Plasmid

    #175095

    Purpose

    13Myc tagged VPS54 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Gershlick et al Hum Mol Genet. 2019 May 1;28(9):1548-156

    Insert

    VPS54 (VPS54 Human)
    

    Use

    Tags

    13Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ACPPiso1-V5

    Plasmid

    #113052

    Purpose

    Expresses human prostatic acid phosphatase (isoform 1) in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

    Insert

    prostatic acid phosphatase isoform 1 (ACP3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ACPPiso2-V5

    Plasmid

    #113053

    Purpose

    Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

    Insert

    prostatic acid phosphatase isoform 2 (ACP3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCA24N_6xHis-TEV-rpsA

    Plasmid

    #83585

    Purpose

    Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

    Depositor

    Nils Walter

    Article

    Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

    Insert

    rpsA (rpsA Escherichia coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    p-T5 Lac

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCI pHluorin Syt2

    Plasmid

    #184503

    Purpose

    Mammalian expression of Synaptotagmin 2 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 2 (Syt2 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEF-pIgH-ESCargo(FTV)

    Plasmid

    #140160

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJH776_pJH_LldR_PlldR-sfGFP.3opal

    Plasmid

    #163735

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding three opal codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.3TGA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gfp with first three leucine codons mutated to am…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SlugABEmax

    Plasmid

    #163798

    Purpose

    Expresses ABEmax and nSlugCas9

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SlugCas9 (D10A)

    Promoter

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Ubiquitin K48C

    Plasmid

    #86588

    Purpose

    Bacterial expression of Ubiquitin K48C for cross linking studies

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K48C

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pJM005

    Plasmid

    #134150

    Purpose

    Retroviral plasmid expressing GFP - Nde1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

    Insert

    Nde1_SSSC (NDE1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SSSC

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Fuw CBX8

    Plasmid

    #121111

    Purpose

    conditional expression

    Depositor

    Emily Dykhuizen

    Article

    Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDOC-K-glmS-GFPfor

    Plasmid

    #158059

    Purpose

    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    Green Fluorescent Protein optimised for excitation with UV light
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    acpP

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

    Plasmid

    #140168

    Purpose

    Expresses a regulatable secretory cargo for Tetrahymena thermophila

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    MTT1

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSIBR004

    Plasmid

    #177667

    Purpose

    SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJM010

    Plasmid

    #134154

    Purpose

    Retroviral plasmid expressing GFP - Nde1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

    Insert

    Nde1_KMLL (NDE1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    KMLL

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. BFP Homology Donor

    Plasmid

    #149372

    Purpose

    Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Insert

    CDDR BFP Homology Donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEF6 VPS52-V5-6His

    Plasmid

    #175096

    Purpose

    V5 tagged VPS52 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

    Insert

    VPS52 (VPS52 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    N666D- please see depositor comment

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRG706_lexO-SbfI_LexA-TF_LEU2MX

    Plasmid

    #154818

    Purpose

    Inducible expression of SbfI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    SbfI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. p-G4PID

    Plasmid

    #209638

    Purpose

    Expression G4PID in mammalian cells

    Depositor

    Xiang Zhou

    Article

    Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

    Insert

    G4PID (DHX36 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

    Plasmid

    #214990

    Purpose

    Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (Eea1 Mouse)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSIBR003

    Plasmid

    #177666

    Purpose

    SIBR Intron 3 (Int3) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. YFP-RPS5-C1

    Plasmid

    #134534

    Purpose

    Expresses YFP-RPS5 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    RPS5 (RPS5 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pTJV1Sc3-rpoB

    Plasmid

    #166983

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    RecT (recT E. coli)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVvanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAID1.2-EF1a-NGFP-mAID

    Plasmid

    #140607

    Purpose

    Expression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3.1-V5-hTEV

    Plasmid

    #65800

    Purpose

    expresses human codon-optimised TEV protease

    Depositor

    Douglas Green

    Article

    Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

    Insert

    V5-human TEV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    codon-optimised to express in mammalian cells

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

48. pUDP293

    Plasmid

    #204228

    Purpose

    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

    Depositor

    Jean-Marc Daran

    Article

    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

    Inserts

    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    Saccharomyces cerevisiae PGK1 and Saccharomyces c…

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pUb RxCas13d + RxCas13d guide RNA

    Plasmid

    #176303

    Purpose

    Expresses RxCas13d and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    RxCas13d
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pTE5400_pMBP_CDS dropout vector

    Plasmid

    #221189

    Purpose

    Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Use

    Tags

    10x His-tag + E. coli maltose binding protein (MB…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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